1. Cellular Cholesterol Regulates Ubiquitination and Degradation of the Cholesterol Export Proteins ABCA1 and ABCG1
- Author
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Wendy Jessup, Cecilia Sandoval, Andrew J. Brown, Victar Hsieh, Maaike Kockx, Mathew Traini, Leonard Kritharides, Mi-Jurng Kim, Ingrid C. Gelissen, and Jeannette C. Hallab
- Subjects
Proteasome Endopeptidase Complex ,CHO Cells ,Protein degradation ,Biology ,Cholesterol 7 alpha-hydroxylase ,Biochemistry ,Cell Line ,Cricetulus ,polycyclic compounds ,Animals ,Humans ,cardiovascular diseases ,Molecular Biology ,ATP Binding Cassette Transporter, Subfamily G, Member 1 ,Ubiquitin ,Macrophages ,Ubiquitination ,nutritional and metabolic diseases ,hemic and immune systems ,Biological Transport ,Cell Biology ,Atherosclerosis ,Lipids ,Transport protein ,Cell biology ,Cholesterol ,Cholesterol transporter activity ,ATP Binding Cassette Transporter 1 ,Proteasome ,Membrane protein ,ABCA1 ,biology.protein ,ATP-Binding Cassette Transporters ,lipids (amino acids, peptides, and proteins) ,Proteasome Inhibitors - Abstract
The objective of this study was to examine the influence of cholesterol in post-translational control of ABCA1 and ABCG1 protein expression. Using CHO cell lines stably expressing human ABCA1 or ABCG1, we observed that the abundance of these proteins is increased by cell cholesterol loading. The response to increased cholesterol is rapid, is independent of transcription, and appears to be specific for these membrane proteins. The effect is mediated through cholesterol-dependent inhibition of transporter protein degradation. Cell cholesterol loading similarly regulates degradation of endogenously expressed ABCA1 and ABCG1 in human THP-1 macrophages. Turnover of ABCA1 and ABCG1 is strongly inhibited by proteasomal inhibitors and is unresponsive to inhibitors of lysosomal proteolysis. Furthermore, cell cholesterol loading inhibits ubiquitination of ABCA1 and ABCG1. Our findings provide evidence for a rapid, cholesterol-dependent, post-translational control of ABCA1 and ABCG1 protein levels, mediated through a specific and sterol-sensitive mechanism for suppression of transporter protein ubiquitination, which in turn decreases proteasomal degradation. This provides a mechanism for acute fine-tuning of cholesterol transporter activity in response to fluctuations in cell cholesterol levels, in addition to the longer term cholesterol-dependent transcriptional regulation of these genes.
- Published
- 2014
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