1. Structural Basis for Selective Recognition of Pneumococcal Cell Wall by Modular Endolysin from Phage Cp-1
- Author
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Juan A. Hermoso, Pedro García, Armando Albert, Martín Martínez-Ripoll, Beatriz Galán, José Luis García, Oussama Ahrazem, Margarita Menéndez, Begoña Monterroso, Dirección General de Investigación Científica y Técnica, DGICT (España), Comunidad de Madrid, Hermoso, Juan A., Monterroso, Begoña, Albert, Armando, Galán, Beatriz, Ahrazem, Oussama, García, Pedro, Martínez-Ripoll, Martín, García, José Luis, Menéndez, Margarita, Hermoso, Juan A. [0000-0002-1862-8950], Monterroso, Begoña [0000-0003-2538-084X], Albert, Armando [0000-0002-0026-6816], Galán, Beatriz [0000-0002-2596-6034], Ahrazem, Oussama [0000-0001-9183-9319], García, Pedro [0000-0001-6717-8717], Martínez-Ripoll, Martín [0000-0002-4019-0529], García, José Luis [0000-0002-9238-2485], and Menéndez, Margarita [0000-0002-3267-4443]
- Subjects
Binding Sites ,Stereochemistry ,Mutagenesis ,Lysin ,Beta sheet ,N-Acetylmuramoyl-L-alanine Amidase ,Peptidoglycan ,Biology ,Enzybiotics ,Choline ,Protein Structure, Tertiary ,chemistry.chemical_compound ,Streptococcus pneumoniae ,chemistry ,Biochemistry ,Cell Wall ,Structural Biology ,Hydrolase ,Endopeptidases ,Glycoside hydrolase ,Bacteriophages ,Muramidase ,Choline binding ,Molecular Biology - Abstract
11 p.-5 fig.-2 tab., Pneumococcal bacteriophage-encoded lysins are modular choline binding proteins that have been shown to act as enzymatic antimicrobial agents (enzybiotics) against streptococcal infections. Here we present the crystal structures of the free and choline bound states of the Cpl-1 lysin, encoded by the pneumococcal phage Cp-1. While the catalytic module displays an irregular (β/α)5β3 barrel, the cell wall-anchoring module is formed by six similar choline binding repeats (ChBrs), arranged into two different structural regions: a left-handed superhelical domain configuring two choline binding sites, and a β sheet domain that contributes in bringing together the whole structure. Crystallographic and site-directed mutagenesis studies allow us to propose a general catalytic mechanism for the whole glycoside hydrolase family 25. Our work provides the first complete structure of a member of the large family of choline binding proteins and reveals that ChBrs are versatile elements able to tune the evolution and specificity of the pneumococcal surface proteins., This work was supported by grants BIO2000-1307 and BIO2002-02887 from Dirección General de Investigación and by grant of Contrato-Programa de Grupos Estratégicos (BMC2000-1002) de la Comunidad Autónoma de Madrid.
- Published
- 2003
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