Search

Cellular agriculture could represent a more sustainable alternative to current food and nutraceutical production processes. Tisochrysis lutea microalgae represents a rich source of antioxidants and omega-3 fatty acids essential for human health. However, current downstream technologies are limiting its use. The present work investigates mild targeted acoustic treatment of Tisochrysis lutea biomass at different growth stages and acoustic frequencies, intensities and treatment times. Significant differences have been observed in terms of the impact of these variables on the cell disruption and energy requirements. Lower frequencies of 20 kHz required a minimum of 4500 J to disrupt 90% of the cells, while only 1000 J at 1146 kHz. Comparing these results with current industry standards such as bead milling, up to six times less energy use has been identified. These mild biomass processing approaches offer a certain tunability which could suit a wide range of microorganisms with only minor adjustments.

Mathematical models were developed to predict biomass and hydrocarbon productivities and colony size (ouputs) of Botryococcus braunii showa cultures based on light intensity, temperature and dilution rate (inputs). These models predicted the following maximum values: biomass productivity, 1.3 g L−1 d−1; hydrocarbon productivity, 1.5 mg L−1 d−1; colony size, 320 µm under different culture conditions respectively. These values were confirmed experimentally. Additionally, the combination of inputs that simultaneously maximize all the possible outputs combinations were determined. The prediction for biomass-hydrocarbon-colony size were 1 g L−1 d−1, 12.05 mg L−1 d−1 and 156.8 µm respectively; biomass productivity-hydrocarbon productivities: 1 g L−1 d−1 and 13.94 mg L−1 d−1 respectively; biomass productivity-colony size: 1 g L−1 d−1 and 172.8 µm respectively; hydrocarbon productivity-colony size: 9 mg L−1 d−1 and 240 µm respectively. All these predictions were validated experimentally. These models might be very useful to implement a Botryococcus braunii showa large scale production. publishedVersion

International audience; Microalgae are promising natural resources for biofuels, chemical, food and feed products. Besides their economic potential, the environmental sustainability must be examined. Cultivation has a significant environmental impact that depends on reactor selection and operating conditions. To identify the main environmental bottlenecks for scale-up to industrial facilities this study provides a comparative life cycle assessment (LCA) of open raceway ponds and tubular photobioreactors at pilot scale. The results are based on experimental data from real pilot plants operated in summer, fall and winter at AlgaePARC (Wageningen, The Netherlands). The energy consumption for temperature regulation presented the highest environmental burden. The production of nutrients affected some categories. Despite limited differences compared to the vertical system, the horizontal PBR was found the most efficient in terms of productivity and environmental impact. The ORP was, given the Dutch climatic conditions, only feasible under summer operation. The results highlight the relevance of LCA as a tool for decision-making in process design. Weather conditions and availability of sources for temperature regulation were identified as essential factors for the selection of geographic locations and for microalgal cultivation systems based on environmental criteria. Simulation of large-scale reactors with optimized temperature regulation systems lead to environmental improvements and energy demand reductions ranging from 17% up to 90% for systems operated in favorable summer conditions.

In this work, the mild disintegration of the microalgae Chlorella vulgaris for the release of intracellular products has been studied. By means of bead milling the microalgae suspensions were successfully disintegrated at different biomass concentrations (25-145 gDW kg(-1)) over a range of agitator speeds (6-12 m s(-1)). In all cases over 97% of cell disintegration was achieved resulting in a release of water soluble proteins. A clear optimum rate of disintegration and protein release was observed at an agitator speed of 9-10 m s(-1) regardless of the biomass concentration. Selective extraction of water soluble proteins was observed as proteins released sooner than cell disintegration took place. Proteins could be released at 85% lower energy input than for cell disintegration resulting in specific energy consumptions well below 2.5 kWh kgDW(-1).

Microalgae are a promising source for proteins, lipids and carbohydrates for the food/feed and biofuel industry. In comparison with soya and palm oil, microalgae can be grown in a more efficient and sustainable way. To make microalgae production economically feasible it is necessary to optimally use all produced compounds. To accomplish this focus needs to be put on biorefinery techniques which are mild and effective. Of the techniques described, Pulsed Electric Field (PEF) seems to be the most developed technique compared to other cell disruption applications. For separation technology ionic liquids seems most promising as they are able to both separate hydrophobic and hydrophilic compounds. But additional studies need to be evolved in the coming years to investigate their relevance as novel cell disruption and separation methods. We propose a complete downstream processing flow diagram that is promising in terms of low energy use and state of the art knowledge.

Hydrogen is a clean energy alternative to fossil fuels. Photosynthetic bacteria produce hydrogen from organic compounds by an anaerobic light-dependent electron transfer process. In the present study hydrogen production by three photosynthetic bacterial strains (Rhodopseudomonas sp., Rhodopseudomonas palustris and a non-identified strain), from four different short-chain organic acids (lactate, malate, acetate and butyrate) was investigated. The effect of light intensity on hydrogen production was also studied by supplying two different light intensities, using acetate as the electron donor. Hydrogen production rates and light efficiencies were compared. Rhodopseudomonas sp. produced the highest volume of H2. This strain reached a maximum H2 production rate of 25 ml H2 l(-1) h(-1), under a light intensity of 680 micromol photons m(-2) s(-1), and a maximum light efficiency of 6.2% under a light intensity of 43 micromol photons m(-2) s(-1). Furthermore, a decrease in acetate concentration from 22 to 11 mM resulted in a decrease in the hydrogen evolved from 214 to 27 ml H2 per vessel.