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3. Definition of the transcription initiation site of human plasminogen gene in liver and non hepatic cell lines

Author

Germana Meroni, Nicoletta Malgaretti, Sergio Ottolenghi, Roberto Taramelli, Giulia Candiani, Marco Pontoglio, Ludovica Bruno, Malgaretti, N, Bruno, L, Pontoglio, M, Candiani, G, Meroni, Germana, Ottolenghi, S, and Taramelli, R.

Subjects
RNA Caps, Transcription, Genetic, Molecular Sequence Data, Biophysics, Polymerase Chain Reaction, Biochemistry, Primer extension, Cell Line, HeLa, Plasminogen gene, promoter, Humans, RNA, Messenger, Northern blot, Promoter Regions, Genetic, Molecular Biology, Gene, chemistry.chemical_classification, Messenger RNA, promoter, Base Sequence, biology, Plasminogen, DNA, Cell Biology, biology.organism_classification, Molecular biology, Enzyme, Liver, chemistry, Cell culture, Hepatic stellate cell, Plasminogen gene
Abstract

Summary We have mapped the cap site of the human plasminogen mRNA by primer extension and PCR techniques and found that it is located at position −161 relative to the first ATG, 97 bases upstream to the 5′ end of the previously isolated cDNA clone. Seven human hepatic and non hepatic cell lines and fresh liver cells were tested for human plasminogen mRNA expression: the liver and the liver derived HepG2 cell line represent the major site of plaminogen RNA synthesis while the other cell lines (Hep3B, HeLa, IMR, 293 Caco and SW626) show much lower levels.

Published
1990

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