1. A comparison of Bradyrhizobium strains using molecular, cultural and field studies
- Author
-
M Chauviére, Keng-Hock Pwee, M. Frémont, H.G Diem, T. K. Tan, and Y Prin
- Subjects
Graine ,ADN ,Plant Science ,F30 - Génétique et amélioration des plantes ,Récupération des sols ,Acacia mangium ,Bradyrhizobium ,Génétique ,food and beverages ,General Medicine ,méthode ,Horticulture ,Technique de l'isolement ,Nitrogen fixation ,Rhizobium ,Restriction fragment length polymorphism ,Développement biologique ,Analyse biologique ,Antibiotique ,Biology ,Inoculation ,Symbiosis ,Botany ,Genetics ,Bactérie fixatrice de l'azote ,Ribosomal DNA ,P36 - Érosion, conservation et récupération des sols ,Résistance aux produits chimiques ,biology.organism_classification ,K10 - Production forestière ,Agronomy and Crop Science - Abstract
Acacia mangium# is a leguminous tree used in industrial plantations for the production of pulp wood, and for the rehabilitation of degraded soils. Its ability to grow in very poor soils is due to its symbiotic association with nitrogen-fixing #Bradyrhizobium#. In this study, 33 isolates of #Bradyrhizobium# and #Rhizobium# obtained from #A. mangium# plantations in Sabah, Malaysia, were characterised by PCR amplification of ribosomal DNA and RFLP analysis, host spectrum and antibiotic resistance. The Sabah isolates were also compared with isolates collected from Singapore, Australia, and Africa. The results distinguished two main groups of #Bradyrhizobium#. Group I strains were prevalent, widely dispersed but genetically closely related. They were also closely related to the Singapore and Australian strains. Strains from Group II were less widely dispersed and more distantly related. Greenhouse and field inoculation trials with #A. mangium# seedlings showed that the Group I strains were more effective in enhancing plant growth than Group II strains. (Resume d'auteur)
- Published
- 1999