1. In vivo endoproteolytically cleaved phaseolin is stable and accumulates in developing Phaseolus lunatus L. seeds
- Author
-
Roberto Bollini, Francesca Sparvoli, Lucia Lioi, and M. Gloria Daminati
- Subjects
Glycosylation ,Protein Conformation ,Protein subunit ,Blotting, Western ,Molecular Sequence Data ,Gene Dosage ,Biophysics ,Cross Reactions ,Biology ,Endoplasmic Reticulum ,Genes, Plant ,Biochemistry ,Structural Biology ,Storage protein ,Amino Acid Sequence ,Molecular Biology ,Polyacrylamide gel electrophoresis ,Glycoproteins ,Plant Proteins ,chemistry.chemical_classification ,Plants, Medicinal ,Sequence Homology, Amino Acid ,Molecular mass ,food and beverages ,Fabaceae ,Protein engineering ,Chromatography, Ion Exchange ,biology.organism_classification ,Molecular biology ,phaseolin ,protein engineering ,protein processing ,seed storage protein ,Molecular Weight ,Blotting, Southern ,Phaseolin ,chemistry ,Seeds ,Phaseolus ,Peptides ,Glycoprotein ,Protein Processing, Post-Translational - Abstract
Phaseolin is the most abundant storage protein of bean seeds. To modify its amino-acidic composition by protein engineering, for the improvement of its nutritional value, regions which could be modified without detrimental effects on structural features of the protein must be identified. Data presented here, on the characterisation of the major storage protein of lima bean (Phaseolus lunatus L.) seeds, a phaseolin-like glycoprotein, provide good indications on one of such region. Phaseolus lunatus phaseolin consists of four major oligomers containing two subunit classes. Polypeptides of one class show a molecular mass ranging from 38.5 kDa to 32 kDa, while the molecular mass of polypeptides belonging to the other class ranges from 27 kDa to 21 kDa. The subunits originate from the cleavage of precursor forms, with molecular masses of 58 kDa and 54 kDa, which are still present — in residual amounts — in the mature protein. Comparison of their N-terminal sequences with those of the subunits demonstrate that cleavage occurs in a region of the molecule that instead remains uncleaved in phaseolins of the other species. Since this region can accommodate such a drastic modification, we suggest it could be a good candidate for in vitro manipulation.
- Published
- 1996