1. Differentially Gene Expression Profile Related to Inflammation in Endometrial Cells Induce by Lipopolysaccharide
- Author
-
Chun-mei Li, Sheng Li, Li-hua Qin, and Ruo-guang Wang
- Subjects
medicine.medical_specialty ,Lipopolysaccharide ,RNA ,Inflammation ,Biology ,Molecular biology ,Pathology and Forensic Medicine ,chemistry.chemical_compound ,Endocrinology ,chemistry ,Trizol ,Internal medicine ,Follicular phase ,Agarose gel electrophoresis ,Gene expression ,medicine ,medicine.symptom ,Gene - Abstract
Objective To investigate differentially expressed genes related to inflammation in endometrial cells induced by Lipopolysaccharide(LPS). Methods Normal endometrium in the proliferative phase of specimen from 3 cases for the experiment was collected. The LPS group were treated with 50 µg/ml LPS. Total RNA was extracted using Trizol reagent from cells. RNA quality was assessed by determining the OD260/280 ratio by agarose gel electrophoresis, the chip was scanned by laser scanner. The acquired was analyzed. Results A total of differentially expressed genes were found, these genes were relative to many aspects. Among them, the expression of genes involved in inflammation were up-regulated by LPS, such as overexpression of IL-1β, 8, etc. Conclusion The results indicates that inflammation-related genes may be one of the mechanisms of abnormal uterine bleeding by LPS-induced.
- Published
- 2009
- Full Text
- View/download PDF