1. The Kiss2/GPR54 system stimulates the reproductive axis in male black porgy, Acanthopagrus schlegelii
- Author
-
Xi-Lan Ma, Bao-Lei Yuan, and Li-Bin Zhou
- Subjects
Male ,media_common.quotation_subject ,Zoology ,030209 endocrinology & metabolism ,Hypothalamic–pituitary–gonadal axis ,Biology ,03 medical and health sciences ,0302 clinical medicine ,Endocrinology ,Kisspeptin ,Animals ,Acanthopagrus schlegeli ,Testosterone ,Tissue Distribution ,Amino Acid Sequence ,Receptor ,Gene ,Pcr analysis ,Phylogeny ,030304 developmental biology ,media_common ,chemistry.chemical_classification ,Kisspeptins ,0303 health sciences ,Base Sequence ,Reproduction ,Brain ,Perciformes ,Amino acid ,Gene Expression Regulation ,chemistry ,Animal Science and Zoology ,hormones, hormone substitutes, and hormone antagonists ,Receptors, Kisspeptin-1 - Abstract
Although it is well established that the Kiss1/GPR54 system stimulates the reproductive axis in mammals, its functional roles, especially in male reproduction of non-mammalian species, is less clear. In this study, we have isolated the full-length kiss2 and gpr54 cDNAs from black porgy (Acanthopagrus schlegelii). The Kiss2 precursor expressed from kiss2 comprises 124 amino acids and contains a highly conserved 10-amino acid sequence, Kiss2-10 (FNFNPFGLRF). GPR54 comprises 375 amino acid residues and contains distinct characteristics of G protein-coupled receptors. Real-time PCR analysis indicated that kiss2 and gpr54 were expressed highly in the brain regions. Moreover, intraperitoneal injection of porgy Kiss2-10 could stimulate genes expression of the gpr54, gnrh1, gnrh3, fshβ, lhβ, p450c17, star, and ar, and the serum testerone level in male black porgy. Our findings demonstrate that the Kisspeptin stimulates the male reproductive axis in black porgy.
- Published
- 2019