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From 1991-2014, all major burn centers of the German-speaking countries participated in a multicenter study in which essentially demographic data were collected. Individual patient data was located at the particular burn centers and only cumulated data were summarized annually for presentation. Retrospective statistical analysis of the entire data collection and identification of subgroups was not possible. In 2015 the German Burn Registry was established for prospective collection of individual patient data as a tool for quality management (QM) and for scientific analyses.A working group was formed to ensure the development and administration of the registry. From the official start of the German Burn Registry at the beginning of the year 2015 prospective data collection was realized with an individualized, web-based data collection software in a pseudonymized way. Selected data analysis was performed for the first 5 years of data collection. Severely burned adults and all hospitalized children with burn injuries were documented in the registry.The German Burn Registry was successfully established. 64 burn divisions have already been registered. 18,891 patients were documented over 5 years, of which 58% where children (16 years). Mean ABSI Score was 4 (children: 3, adults: 6) with a mortality rate of 3.8.0% (children: 0.2%, adults: 9.3%). Children were hospitalized for an average of 7 days, whereas adults were discharged after 18 days.A registry is mandatory for quality assurance in burn medicine, since realization of randomized studies is difficult due to the heterogeneity of burn injuries. The German Burn Registry already is one of the biggest burn registries in Europe. Several scientific projects, based on the registry database, are in working process or have already been published.

Impaired microcirculation, along with an increase in chronic medical conditions in the geriatric cohort, may favor the development of soft-tissue defects in the lower extremity and equally impair the options for plastic-reconstructive surgery. In particular, outcome analyses in the increasing patient cohort ≥ 80 years (octogenarians) are limited.Setting 80 years as the cutoff, we conducted an age-related outcome analysis of all patients undergoing free-flap reconstruction of the lower extremity from 2014 to 2020, comprising the American Society of Anesthesiologists (ASA) score and Charlson Comorbidity Index (CCI) as the possible outcome predicting factors.During the study period, a total of 424 free flaps were performed in 385 patients (∅: 54.7 years ± 16.1; range: 9-89), including 19 octogenarians. Compared with the younger patient cohort, there was a significantly higher rate of early flap revision (p = 0.023) and flap loss (p = 0.028). Furthermore, the mean length of hospital (60.6 ± 37.6 vs. 51.1 ± 37.0) and intensive care unit/intermediate care stay (6.5 ± 15.0 vs. 3.5 ± 8.5) was extended (n.s.). The ASA score presented an independent predictor for major surgical [odds ratio (OR): 1.66; p = 0.041) and medical complications (OR: 3.97; p0.001). Neither the CCI nor the ASA served as an independent predictor for total flap loss.Free-flap reconstruction of the lower extremity in octogenarians is associated with a higher risk of flap revision and flap loss. Considering the prolonged immobilization associated with increased morbidity following limb amputation, it presents still a reasonable option to achieve limb salvage in carefully chosen patients. An adequate tool to predict the success of free-flap survival is still unavailable.

During long duration orbital space missions, astronauts experience immune system dysregulation, the persistent reactivation of latent herpesviruses, and some degree of clinical incidence. During planned NASA ‘Artemis’ deep space missions the stressors that cause this phenomenon will increase, while clinical care capability will likely be reduced. There is currently minimal clinical laboratory capability aboard the International Space Station (ISS). The ability to monitor the white blood cell count (WBC) and differential during spaceflight has been an unmet NASA medical requirement, primarily due to a lack of capable hardware. We performed ground and flight validation of a device designed to monitor WBC and differential within minutes from a fingerstick blood sample. This device is miniaturized, robust, and generally compatible with microgravity operations. Ground testing for spaceflight consisted of vibration tolerance, power/battery and interface requirements, electromagnetic interference (EMI), and basic evaluation of sample preparation and operations in the context of spaceflight constraints. The in-flight validation performed aboard the ISS by two astronauts included assessment of three levels of control solution (blood) samples as well as a real time analysis of a fingerstick blood sample by one of the crewmembers. Flight and ground testing of the same lot of control solutions yielded similar total WBC values. There was some select discrepancy between flight and ground data for the differential analysis. However, the data suggest that this issue is due to compromise of the control solutions as a result of storage length before flight operations, and not due to a microgravity-associated issue with instrument performance. This evaluation also yielded lessons learned regarding crewmember training for technique-sensitive small-volume biosample collection and handling in microgravity. The fingerstick analysis was successful and was the first real-time hematology assessment performed during spaceflight. This device may provide an in-mission monitoring capability for astronauts thereby assisting Flight Surgeons and the crew medical officer during both orbital and deep space missions.

Introduction Segmental tibia defects remain challenging for orthopedic surgeons to treat. The aim of this study was to demonstrate bone-related and functional outcomes after treatment of complex tibial bone defects using Ilizarov bone transport with a modified intramedullary cable transportation system (CTS). Patients and Methods We conducted a single-center, retrospective study including all 42 patients treated for tibial bone loss via Ilizarov bone transport with CTS between 2005 and 2018. Bone-related and functional results were evaluated according to the Association for the Study and Application of Methods of Ilizarov (ASAMI) scoring system. Complication and failure rates were determined by the patients’ medical files. Results Patients had a mean age of 45.5 ± 15.1 years. The mean bone defect size was 7.7 ± 3.4 cm, the average nonunion scoring system (NUSS) score was 59 ± 9.5 points, and the mean follow-up was 40.8 ± 24.4 months (range, 13-139 months). Complete bone and soft tissue healing occurred in 32/42 patients (76.2%). These patients had excellent (10), good (17), fair (2), and poor (3) results based on the ASAMI functional score. Regarding bone stock, 19 patients had excellent, 10 good, and 3 fair results. In total, 37 minor complications and 62 major complications occurred during the study. In 7 patients, bone and soft tissue healing occurred after CTS failure with either an induced membrane technique or classic bone transport; 3 patients underwent lower leg amputation. Patients with treatment failure were significantly older (57.6 vs. 41.8 years; p = 0.003). Charlson score and treatment failure had a positive correlation (Spearman's rho 0.43; p = 0.004). Conclusion Bone transport using both intramedullary CTS and Ilizarov ring fixation is viable for treating patients with bone loss of the tibia and complex infection or soft tissue conditions. However, a high number of complications and surgical revisions are associated with the treatment of this severe clinical entity and should be taken into account.

Summary Objective Non-invasive Remote Ischemic Conditioning (RIC) offers an approach to reduce tissue damage in various organs/tissues. Besides attenuation of Ischemia-Reperfusion injury (I/R), beneficial effects on cutaneous microcirculation of free microsurgical flaps have been reported. Given the recency of this technique, there are considerable gaps in the current understanding of its mechanism of action. As a result, clinical transfer of RIC is prolongated in several fields. We aimed to optimize the RIC protocol by examination of different RIC-cycle numbers and its effect on changes of cutaneous microcirculation and duration. Methods 80 subjects were divided into groups (1, 3, 5, 7 RIC cycles). RIC was applied via an inflatable tourniquet. Cutaneous microcirculation was continuously assessed at the contralateral anterior lateral thigh utilizing a ©O2C-device continuously. Results RIC caused significant and sustained changes in microcirculation. Four hours after completion of RIC, a maximum increase of +80.8% (CI 1.395–2.221) in blood flow and +23.5% (CI 1.098–1.372) in tissue oxygen saturation was measured (three-cycle group). A higher number of applied cycles was accompanied with significant higher mean pain. Conclusion Acute improvement of cutaneous microcirculation due to RIC lasted for at least 4 h after completion of the RIC-protocol. Dose-dependent effects of RIC are likely. With regard to the increase in pain, we recommend a RIC protocol of 3 cycles for future clinical application.

Intra-abdominal compartment syndrome (ACS) is a devastating complication in burn patients with a high mortality. Apart from high-volume resuscitation as known risk factor, also mechanical ventilation seems to influence the development of ACS. The TIRIFIC trial is a retrospective, matched-pair analysis. Thirty-eight burn patients with ACS were matched for burned total body surface area (TBSA), age and mechanical ventilation (MV). In contrast to the already published part I addressing fluid resuscitation as a risk factor, the parameters analyzed in part II were maximum and average PEEP and peak pressure levels as well as serum lactate levels and prokinetic therapy. For subgroup-analysis the ACS-group was split up into an early-onset and late-onset ACS-group according to the median time between burn trauma and ACS. The groups were analyzed with a two-sided Mann-Whitney-U-test with significance set at p < 0.05. In the ACS-group all ventilation pressures (maximum and average PEEP and peak pressure levels) were significantly increased compared to control. The subgroup-analysis showed significantly increased maximum PEEP and peak pressure levels in early- and late-onset ACS-groups versus control. However, the average ventilation pressure levels were only increased in the early-onset ACS-group (average PEEP p = 0.0069; average peak pressure p = 0.05). The TIRIFIC trial showed significantly increased ventilation pressures in the ACS group in general as a surrogate parameter to support early diagnostics. Especially, maximum PEEP levels and peak pressures are significantly increased in both, early- and late-onset ACS. As an addition to the actual WSACS guidelines we suggest IAP measurement in mechanically ventilated burn patients if ventilating pressures are rising continuously without a clear pulmonary or otherwise identifiable reason.

Summary Background Lipedema is a condition of painful increase in subcutaneous fat affecting almost exclusively women. Several studies have examined the effectiveness of liposuction in the treatment of lipedema, but none has focused on water-jet-assisted liposuction technique. Methods A standardized treatment protocol for liposuction in lipedema, which was established over the course of 15 years, is presented. Patients received questionnaires preoperatively and after operative treatment assessing characteristics and symptom severity on visual analog scales in a prospective manner. Results Pre- and postoperative questionnaires were available for 63 patients. Median age was 35 years and mean (body mass index) BMI 28.4 ± 0.6, all patients had stages I or II lipedema diagnosed by two separate specialists. After a mean follow-up of 22 months after operative treatment, all assessed symptom had decreased significantly in severity. All patients wore compression garments and/or received manual lymphatic drainage preoperatively; this could be reduced to only 44% of patients needing any conservative treatment postoperatively. Conclusion Liposuction in water-jet-assisted technique using the presented treatment protocol is an efficient method of operative treatment of early-stage lipedema patients leading to a marked decrease in symptom severity and need for conservative treatment.

Background Soft tissue sarcomas (STS) of the extremities or trunk often require plastic reconstructive transfer of vital tissue for wound closure after resection. Literature on the oncologic outcome of patients receiving flap closure in comparison with patients receiving primary wound closure is very limited. Methods Patients who underwent resection of a primary extremity or truncal STS without dissemination at our institution between January 2000 until December 2015 were eligible for the study. Patients were divided into two groups based on type of soft tissue closure (primary or flap) while patients receiving skin grafting were excluded. Characteristics, oncologic outcome and prognostic factors of both groups were compared. Results 781 patients could be included, of these 200 had received flap closure and 581 primary wound closure. Tumors receiving flap closure were significantly smaller but were located in distal extremities and showed a trend towards prior neoadjuvant radiotherapy. Incidence of wound and general complications was similar in both groups. 5-year local recurrence free survival (LRFS, 71% vs. 69%) and 5-year disease specific survival (DSS, 84% vs. 88%) did not differ significantly between patients with primary closure and flap closure. Most important predictors in both groups were tumor size and grading with no major differences in analysis of predictors for both endpoints. Conclusion Plastic reconstructive surgery plays an important role in limb-conserving STS treatment. Complication rates of patients with flap coverage are not higher than of patients with primary wound closure and oncologic outcome is similar with no major differences in predictors of LRFS and DSS.

In various oncological conditions, complications correlate with diminished prognosis, however literature on soft tissue sarcomas is limited and inconclusive. The aim of this study was to assess risk factors and the oncologic impact of wound complications in primary extremity soft-tissue sarcomas.Patients with primary extremity soft tissue sarcomas without dissemination and with clear surgical margins (R0) were analyzed. Groups with and without wound complications were compared by univariate and multivariable analysis to identify risk factors. Uni- and multivariable analysis of factors associated with local recurrence free survival (LRFS), metastasis free survival (MFS) and disease specific survival (DSS) were performed.682 patients were included in the study, wound complications occurred in 94 patients (13.7%) within 90 days. Age, ASA-stage, high tumor size and grade, tumor location in the foot, neoadjuvant radiation therapy and operation time represented independent risk factors for wound complications. Patients with wound complications had a significantly worse estimated 5-year LRFS of 49.4 ± 6% versus 78.3 ± 2.1% and 5-year DSS of 77.9 ± 5.4% versus 89.1 ± 1.6%. Wound complications could be identified as an independent risk factor for worse LRFS (HR 2.68[CI 1.83-3.93], p 0.001) and DSS (HR 1.79[CI 1.01-3.16], p = 0.046).Wound complications after soft tissue sarcomas of the extremities are associated with worse local oncological outcome and survival. Patients with high risk of wound complications should be identified and strategies implemented to reduce surgical complications and possibly improve oncologic prognosis.

The aim of the study was to investigate the relationship of BMI and abdominal wall thickness (AWT) with the diameter of the dominant deep inferior epigastric artery perforator (DIEP) as well as DIEA branching pattern in preoperatively performed computed tomography angiography (CTA).We conducted a retrospective study including all patients undergoing DIEP flap breast reconstruction with available CTAs from November 2013 to April 2018 in our department. The caliber-strongest DIEP was detected after passage of the rectus fascia as well as the superficial inferior epigastric artery (SIEA) and correlated with the AWT 5 cm above and below the umbilicus, lateral at the level of the umbilicus and at the level of the anterior superior iliac spine (ASIS), and with BMI.Seventy-seven patients met the inclusion criteria (age: 47.3 ± 8.9 years). We observed a significant relationship (p 0.05) between DIEP (mean ∅ = 2.98 mm) and BMI (r = 0.353), the AWT supra- and infraumbilical (r ≥ 0.32), and the AWT lateral at the level of the umbilicus and ASIS (r ≥ 0.25). In addition, there was a highly significant correlation (p 0.01) between SIEA and BMI (r = 0.389) and between the AWT lateral at the level of ASIS (r ≥ 0.41).We demonstrated a correlation of the diameter of the dominant DIEP with both BMI and AWT. Focusing on the diameter, in patients with a high AWT at the level of the ASIS, the SIEA, if present, may represent an alternative therapeutic option.

In order for astronauts to live and work in outer space, environmental conditions must be maintained within very strict parameters. Any change in these conditions (such as atmospheric composition, pressure, and/or temperature) could pose a risk to the astronauts and ultimately to the success of the mission itself. In particular, changes in pressure could place the astronaut at risk for decompression illness (DCI), a potentially life-threatening condition related to the presence of nitrogen bubbles within the body. There are a number of strategies in human spaceflight designed to minimize the risk of DCI or treat it if necessary. However, as humans prepare again to travel beyond Low Earth Orbit (LEO), the risk of developing DCI may increase, as would the difficulties in treating it, given that evacuation to Earth will become substantially less feasible. Even though hyperbaric oxygen therapy (HBOT) is the accepted gold standard for the treatment of DCI on Earth, there is currently limited capacity for this treatment option in space. Expanding HBOT capacity in human spaceflight could prove useful, given that this medical capability can also be used for the treatment of a number of other potentially life-threatening conditions, such as carbon monoxide poisoning, thermal burns, and gangrene. This paper will examine the current issues related to HBOT in space and discuss the opportunities for its use in future human spaceflight endeavors.

Introduction Brugada syndrome (BrS) is a rare cause of sudden cardiac death in young, otherwise healthy individuals with a structurally normal heart. The diagnosis of BrS is made by typical ECG changes (so-called “coved” type 1-ECG) that may be transient and can be unmasked by sodium-channel-blocking medications. Once the pathognomonic ECG pattern is identified, potentially aggravating drugs have to be discontinued. Since many drugs in psychiatric indications have the potential to induce Brugada-like ECG changes, therapy in such patients is challenging and a rapid worsening of depressive or psychotic symptoms can be the consequence. The limited remaining psychopharmacological treatment options may lead to the consideration of electroconvulsive therapy (ECT). Methods In a patient with treatment-resistant major depression and high risk of suicide, a Brugada type 1-ECG has been recorded during routine control under antidepressant pharmacotherapy. We report on the successful treatment using ECT after establishing an interdisciplinary safety precautions protocol for patients with suspected BrS, involving cardiologists, anesthesiologists and psychiatrists. Results Specific considerations of the safety precautions protocol include choice of anesthetic agents, advanced cardiac monitoring, specific emergency prearrangements and the ECT procedure itself. The type of muscle relaxant chosen and the autonomic response on the seizure stimulation were considered most important to avoid ECT-related adverse events. Conclusion ECT might be a promising option in the treatment of severe depressive episodes in patients presenting with a Brugada ECG, if an interdisciplinary risk assessment and safety precautions are carefully considered.

Oscillating rolling element bearings can be found in various industrial applications. A popular example are the rotor blade bearings of wind turbines, which allow the rotor blades to turn around their longitudinal axis. These bearings are predominantly grease lubricated and expected to be running in a state of low velocity starvation. Elastohydrodynamic Lubrication (EHL) films in starved contacts are susceptible to the conditions of inlet lubricant supply. If the bearings are running under moderate starvation no early failure due to wear is expected. If the inlet conditions increasingly lead to a drying out of the contact, resulting in a heavily starved contact, metal-to-metal contact can lead to false brinelling and subsequent bearing failure. Using two different greases bearing experiments are used to gain an understanding of the mechanism of wear initiation. It is found that starvation seems to be a major contribution to wear appearing in the investigated operating conditions (2°-45° osc. angle, 0,2–5 Hz osc. frequency). The degree of starvation in the contact depends on the balance between lubricant displacement by the rolling element and the replenishment of lubricant during operation. The following conclusions could be drawn: Replenishment is highly dependent on the operating conditions such as the oscillation frequency, the oscillation amplitude, and lubricant rheology. For small oscillation angles the ability of the grease to release base oil with high mobility into the contact seams to be essential. For greater oscillation angles, after crossing a limiting angle, a secondary replenishment mechanism seems to become active. This prevents early wear initiation. At critical operating parameters, which mainly include high oscillation frequencies and medium oscillation angles, severe wear is initiated after just a few hundred oscillation cycles. By modifying an existing starvation factor the influences could be visualised and compared to the experimental results.

Negative Pressure Wound Therapy (NPWT) is widely used across different kinds of surgical disciplines. A controversial debate was raised by diverging results from studies that were conducted to examine the impact of NPWT on local perfusion. Thus, there is a lack of evidence for one important underlying factors that influences the physiology of wound healing under an applied NPWT-dressing.To investigate the immediate local perfusion changes due to an applied intermittent NPWT protocol.A NPWT dressing was applied to the antero-lateral thigh of seven healthy volunteers with two probes of both pressure and microcirculatory measuring devices. One of each probe was placed under the NPWT dressing, the other one in close proximity next to it. A protocol consisting of two cycles of 10 min of -125 mmHg pressure, followed by 10 min of 0 mmHg pressure was applied. Measurements of local pressure to the underlying tissue, as well as microcirculatory changes were performed continuously.Applied vacuum caused compressional forces (27.33 mmHg, p 0.05) towards the underlying tissue. Blood Flow was increased after both suction periods (+52.5%, +108.7%; p 0.05) and continued increasing until the end of measurements (+145.3%). This was accompanied by significant increase in Oxygen Saturation (+21.6%; p 0.05) and Relative Hemoglobin Content (+16.7%). Red Blood Cell Velocity was found to be increased without statistical significance. Next to the dressing, changes were also significant but less pronounced.Intermittent NPWT improves local microcirculation with consecutive enhancement of oxygen supply.

Hand injuries are common and can result in a long time off work. To analyse and identify factors affecting time of work, a holistic view on patients is needed. World Health Organization's International Classification of Functioning, Disability and Health (ICF) with its bio-psycho-social perspective provides such a holistic view. The purpose of this study is to analyse time off work in patients with traumatic hand injuries and to identify factors affecting time off work from a bio-psycho-social perspective. We used factors derived from the ICF Core Set for Hand Conditions to predict time off work by applying Cox regression analyses and Kaplan-Meier method using data of a multicentre prospective study in nine German Level 1 hand trauma centres. In total, 231 study participants with a broad range of hand injuries were included. From these, 178 patients (77%) returned to work within 200 days. Impairments in mobility of joint functions and sensory functions related to temperature and other stimuli as well as higher hand strain at work led to extended time off work. Gender, fine hand use and employment status additionally influenced time off work in sub-models. Our results demonstrate that a bio-psycho-social perspective is recommended when investigating time off work.

Background: Severe burns in pregnant women present an exceedingly challenging situation due to the limitation in therapeutic options including pain management and operative care.Case: We present a 30 years old patient, 38th week of pregnancy, who suffered a full thickness skin burns in 20% total body surface area and an inhalation trauma. In a Level-1 trauma center an interdisciplinary team of plastic surgeons, neonatologists and gynecologists was organized to deliver best patient care. Conclusion: This case presents a challenging situation with an intubated analgosedated pregnant woman, limiting treatment options. On admission a prompt cesarean delivery was performed to guard the unborn child. To shorten the hospital stay, a novel Bromelain based enzymatic debridement was performed on the full thickness burn wounds with immediate skin grafting. Keywords: Burn, Pregnancy, Management, Caesarean delivery

Abstracts The slow hydrogen sulphide (H 2 S) releasing compound S -allyl glutathione disulfide (GSSA) from garlic, which can release H 2 S via α carbon substitution, showed anti-aging effects on the chronological lifespan (CLS) of yeast Saccharomyces cerevisiae . Hormesis was observed, showing lifespan extension at nanomolar concentrations (100–200 nM), whereas at high concentration (1 mM) toxicity were observed. A possible explanation therefore might be the beneficial properties of the recently discovered gasotransmitter hydrogen sulfide (H 2 S). Yet, 2-vinyl-4H-1,3-dithiin (2-VDT) and 3-vinyl-4H-1,2-dithiin (3-VDT) from garlic appeared both inactive in terms of prolonging the lifespan of yeast. While for 2-VDT these findings seem reasonable since it cannot release H 2 S, for 3-VDT the contrary was expected as H 2 S releasing properties were observed. The underlying mechanism of H 2 S release of 3-VDT was proposed to be a similar α carbon substitution-like observed for GSSA.

Spreading from the Near East in the declining Bronze Age from the 2nd millennium BCE onwards, the technique of iron smelting reached Eastern Silesia, Poland, in approximately the 2nd century BCE (pre-Roman Iron Age). At this time the region of the Widawa catchment area was inhabited by the Przeworsk culture. While the older moraine landscape of the study area lacks ores from geological rock formations, bog iron ores were relatively widespread and, due to their comparatively easy accessibility, were commonly exploited for early iron production. This paper investigates the mineralogical and elemental composition of local bog iron ore deposits and iron slag finds, as a by-product of the smelting process, also taking into account the state of the art in research regarding the formation, distribution and utilization of bog iron ores and considering data from comparative studies. The crystalline mineralogical composition of local bog iron ores is dominated by quartz (SiO 2 ) and goethite (α-FeO(OH)), in contrast to slag samples in which fayalite (Fe 2 SiO 4 ), wustite (FeO) and quartz, with traces of goethite, represent the main minerals. Ores and slags are both characterized by notable hematite (Fe 2 O 3 ), magnetite (Fe 3 O 4 ) and maghemite (γ-Fe 2 O 3 ) contents. Analyzed bog iron ore samples show iron contents of up to 64.9 mass% Fe 2 O 3 (equivalent to 45.4 mass% Fe), whereas the iron contents of bloomery slags vary between 48.7 and 72.0 mass% FeO (equivalent to 37.9 and 56.0 mass% Fe). A principal component analysis of the element contents, which were quantified by portable energy-dispersive X-ray fluorescence spectrometry, indicates local variations in the elemental composition. The results of this study show that bog iron ores are relatively widely distributed with spatially varying iron contents along the Widawa floodplain but present-day formation conditions, such as changed groundwater levels, are negatively affected by modern land-use practices, such as agriculture and melioration measures.

Many features of the space environment cause physical ailments for human explorers, some which are truly unique. For example, the long-term health effects of living and working in a microgravity environment can currently only be experienced in an orbiting spacecraft. Radiation exposure, however, is a significant concern in space but is also an issue in certain terrestrial environments. Despite similarities with terrestrial radiation, space-based radiation is rarely encountered in an Earth environment. In fact, there are only a few locations around the world where space radiation can even be produced for research purposes. Although many long-term studies on the health effects of terrestrial radiation have been performed, there remain significant uncertainties as to whether or not Earth-based radiation can be used as a model for space-based radiation. Some of this uncertainty rests with the limited human-applicable radiation data acquired in space environments beyond Low Earth Orbit. Recent publications documenting radiation measurements from NASA’s Mars Science Laboratory have significantly added to the understanding of estimated total radiation exposure doses during a human Mars mission. Despite the uncertainties regarding these estimates and the use of Earth-based radiation as a model, it is known that there are unquestionable health risks associated with long-term exposure to space radiation including tissue damage, increased cancer risk, acute radiation syndrome, central nervous system defects, and many others. This paper will discuss these health risks, the differences between terrestrial and space radiation, recent knowledge developments regarding space radiation, and also potential countermeasures for protecting future human spaceflight explorers.

Edema formation due to the collapse of physiological barriers and the associated delayed healing process is still a central problem in the treatment of burn injuries. In healthy individuals, tight junctions form a barrier to fluid and small molecules. Cingulin is a cytoplasmic component of tight junctions and is involved in the regulation of the paracellular barrier. Endothelial specific cingulin knock-out mice provide new insight into the influence of tight junction proteins on edema formation and angiogenesis during wound healing. Knock-out mice lacking the head domain of cingulin in endothelial cells (CgnΔEC) were created by breeding Cgnfl/fl mice with Tie1-cre mice. Using a no-touch hot air jet a burn trauma was induced on the ear of the mouse. Over a period of 12 days microcirculatory parameters such as edema formation, angiogenesis and leukocyte-endothelial interactions were visualized using intravital fluorescence microscopy. At baseline, CgnΔEC mice surprisingly showed significantly less tracer extravasation compared to Cgnfl/fl littermates, whereas, after burn injury, edema was consistently higher in CgnΔEC mice. Non-perfused area after wounding was increased, but there was no difference in vessel diameters, contraction or dilation of arteries in CgnΔEC mice. Moreover, cingulin knock-out did not cause a difference in leukocyte adhesion after burn injury. In summary, cingulin limits non-perfused area after burn injury and maintains the paracellular barrier of blood vessels. Since edema formation with serious systemic effects is a central problem of burn wounds, understanding the importance of tight junction proteins might help to find new treatment strategies for burn wounds.

This article describes a mass spectrometry data set generated from osteogenic differentiated bone marrow stromal cells (BMSCs) and adipose tissue derived stromal cells (ASCs) of a 24-year old healthy donor. Before osteogenic differentiation and performing mass spectrometric measurements cells have been characterized as mesenchymal stromal cells via FACS-analysis positive for CD90 and CD105 and negative for CD14, CD34, CD45 and CD11b and tri-lineage differentiation. After osteogenic differentiation, both cell types were homogenized and then fractionated by SDS gel electrophoresis, resulting in 12 fractions. The proteins underwent an in-gel digestion, spiked with iRT peptides and analysed by nanoHPLC-ESI-MS/MS, resulting in 24 data files. The data files generated from the described workflow are hosted in the public repository ProteomeXchange with identifier PXD015026. The presented data set can be used as a spectral library for analysis of key proteins in the context of osteogenic differentiation of mesenchymal stromal cells for regenerative applications. Moreover, these data can be used to perform comparative proteomic analysis of different mesenchymal stromal cells or stem cells upon osteogenic differentiation. In addition, these data can also be used to determine the optimal settings for measuring proteins and peptides of interest. Keywords: Proteome, Spectral peptide library, Mesenchymal stromal cells, Adipose derived stromal cells, Bone marrow derived stromal cells, Osteogenic differentiation, Osteogenesis