1. Detection of synergistic combinations of Baccharis extracts with Terbinafine against Trichophyton rubrum with high throughput screening synergy assay (HTSS) followed by 3D graphs. Behavior of some of their components
- Author
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Susana Zacchino, Maximiliano Sortino, Juan J. Ivancovich, Marcela Raimondi, Laura S. Favier, José M. Pellegrino, María Victoria Rodriguez, and Martha Gattuso
- Subjects
Antifungal Agents ,Antifungal drug ,Pharmaceutical Science ,Trichophyton rubrum ,Pharmacology ,High-performance liquid chromatography ,chemistry.chemical_compound ,Trichophyton ,Drug Discovery ,Chromatography, High Pressure Liquid ,Additivism ,Molecular Structure ,biology ,Baccharis ,Drug Synergism ,Bioquímica y Biología Molecular ,3D surface-response curves ,Molecular Medicine ,Growth inhibition ,CIENCIAS NATURALES Y EXACTAS ,medicine.drug ,Spectrometry, Mass, Electrospray Ionization ,Ent-clerodanes ,High-throughput screening ,Argentina ,High Throughput Screening Synergy Assay ,Microbial Sensitivity Tests ,Naphthalenes ,Antifungal ,CombiTool software ,Diterpenes, Clerodane ,Ciencias Biológicas ,medicine ,Baccharis species ,Terbinafine ,Flavonoids ,Chromatography ,Dose-Response Relationship, Drug ,Plant Extracts ,Synergism ,Plant Components, Aerial ,biology.organism_classification ,High-Throughput Screening Assays ,Complementary and alternative medicine ,chemistry ,Software - Abstract
Forty four extracts from nine Baccharis spp. from the Caulopterae section were tested in combination with terbinafine against Trichophyton rubrum with the HTSS assay at six different ratios with the aim of detecting those mixtures that produced a ≥50%statistically significant enhancement of growth inhibition. Since an enhanced effect of a combination respective of its components, does not necessarily indicate synergism, three-dimensional (3D) dose–response surfaces were constructed for each selected pair of extract/antifungal drug with the aid of CombiTool software. Ten extracts showed synergistic or additive combinations which constitutes a 22% hit rate of the extracts submitted to evaluation. Four flavonoids and three ent-clerodanes were detected in the active Baccharis extracts with HPLC/UV/ESI-MS methodology, all of which were tested in combination with terbinafine. Results showed that ent-clerodanes but not flavonoids showed synergistic or additive effects. Among them, bacchotricuneatin A followed by bacrispine showed synergistic effects while hawtriwaic acid showed additive effects. Fil: Rodriguez, María Victoria. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Sortino, Maximiliano Andrés. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ivancovich, Juan J.. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Pellegrino, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Fisiología Experimental. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Fisiología Experimental; Argentina Fil: Favier, Laura Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina Fil: Raimondi, Marcela P.. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina Fil: Gattuso, Martha Ana. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Zacchino, Susana Alicia Stella. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
- Published
- 2013