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4 results on '"Laboratoire Hospitalo-Universitaire de Parasitologie-Mycologie"'

1. Molecular characterization of Rhodnius robustus specimens, potential vectors for Chagas disease in French Guiana, South America

Author

Denis Blanchet, Christian Barnabé, Christine Aznar, Jean-François Guégan, Simone Frédérique Brenière, Interactions hôtes-vecteurs-parasites-environnement dans les maladies tropicales négligées dues aux trypanosomatides (UMR INTERTRYP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université de Bordeaux (UB), Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Ecosystemes Amazoniens et Pathologie Tropicale (EPat), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Guyane (UG), Laboratoire Hospitalo-Universitaire de Parasitologie-Mycologie, and Coordination Régionale de la lutte contre le Virus de L'Immunodéficience Humaine (COREVIH)-Centre Hospitalier Andrée Rosemon [Cayenne, Guyane Française]-Université des Antilles (UA)

Subjects
0301 basic medicine, Microbiology (medical), Chagas disease, Nuclear gene, 030231 tropical medicine, Rhodnius, Genes, Insect, Biology, Microbiology, 03 medical and health sciences, 0302 clinical medicine, Haplotype distribution, Rhodnius robustus, Genus, Genetics, Animals, Chagas Disease, Molecular Biology, ComputingMilieux_MISCELLANEOUS, Ecology, Evolution, Behavior and Systematics, Phylogenetic tree, Cytochrome b, Cytochrome B, Haplotype, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, biology.organism_classification, French Guiana, Insect Vectors, 030104 developmental biology, Infectious Diseases, Haplotypes, Evolutionary biology, Genetic structure, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, Taxonomy (biology)
Abstract

Insects of the genus Rhodnius are broadly involved in Chagas disease transmission. In French Guiana, where the disease remains a public health problem, R. robustus and R. pictipes are vectors, but so far few genetic analyses of these local species have been reported. Here, we explored three mitochondrial genes (Cytb, Lsu-rRNA, and ND1) and one nuclear gene (D2) in 49 adult specimens morphologically characterized as R. robustus. We analyzed genetic polymorphisms and haplotype distributions, and we built phylogenetic trees using the available GenBank sequences from R. robustus and related species. The molecular taxonomy analysis confirmed that 35 insects, closely related to Brazilian species and separated by a few mutations, truly belong to R. robustus; two others were attributed to the R. prolixus complex and for 12 no sequence was obtained. The geographical haplotype distribution indicates a likely geographical structuring and evidenced true differentiation between the two main urban centers, Cayenne and Saint-Laurent-du-Maroni.

Published
2018
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2. Leishmaniavirus genetic diversity is not related to leishmaniasis treatment failure

Author

Sébastien Briolant, Marine Ginouves, Eliane Bourreau, Magalie Demar, Paul Brousse, S. Simon, S. Rogier, P. Travers, V. Pommier de Santi, Pierre Couppié, Ghislaine Prévot, Ecosystemes Amazoniens et Pathologie Tropicale (EPat), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Guyane (UG), Centre National de Référence Leishmania [Cayenne, Guyanne Française] (Laboratoire Associé), Laboratoire Hospitalo-Universitaire de Parasitologie-Mycologie, Coordination Régionale de la lutte contre le Virus de L'Immunodéficience Humaine (COREVIH)-Centre Hospitalier Andrée Rosemon [Cayenne, Guyane Française]-Université des Antilles (UA)-Coordination Régionale de la lutte contre le Virus de L'Immunodéficience Humaine (COREVIH)-Centre Hospitalier Andrée Rosemon [Cayenne, Guyane Française]-Université des Antilles (UA), Service de Dermatologie et Vénérologie [CH de Cayenne, France], Centre Hospitalier Andrée Rosemon [Cayenne, Guyane Française], Institut Pasteur de la Guyane, Réseau International des Instituts Pasteur (RIIP), Centre d'Investigation Clinique Antilles-Guyane (CIC - Antilles Guyane), Université des Antilles et de la Guyane (UAG)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pointe-à-Pitre/Abymes [Guadeloupe] -CHU de Fort de France-Centre Hospitalier Andrée Rosemon [Cayenne, Guyane Française], Département des Centres Délocalisés de Prévention et de Soins [Cayenne, Guyane Française], Pôle des Centres Délocalisés de Prévention et de Soins [CH Andrée Rosemon, Guyane Française], Vecteurs - Infections tropicales et méditerranéennes (VITROME), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées [Brétigny-sur-Orge] (IRBA), Coordination Régionale de la lutte contre le Virus de L'Immunodéficience Humaine (COREVIH)-Centre Hospitalier Andrée Rosemon [Cayenne, Guyane Française]-Université des Antilles (UA), This work was supported by the University of French Guiana and the Ministère Français de l'Enseignement Supérieur et de la Recherche Scientifique., This work has benefited from an 'Investissement d’Avenir' grant managed by Agence Nationale de la Recherche (CEBA, ref. ANR-10-LABX-25-01)., We thank all trainees who participated in this project at the level of experimental work (Claudiane FLORAT, Yasmine AUGUSTE, Ruthly JEAN-BAPTISTE, Keïta BAGADI, Kévin JOSEPH). We also thank all CHC staff (Audrey Ambouille, Géraldine Gangasing, Philippe Travers, Basma Guarmit, Céline Michaud) and health centers staff (Maripasoula: Christelle Maabo, Wilmina Alfred, Melaine Boce and their colleagues, Papaichton: Naïka Amayota and colleagues, in Cacao: Malika Miquel and colleagues, in Saint Georges: Eric Crougneau and colleagues, in Apatou: Pauline Alifons and colleagues, in Saül: Séverine and colleagues, in Trois sauts: Benoit Quentin, Laure Gerold and colleagues) who helped us to carry out this project. We thank Antoine Adenis for his help in the sensitivity study. We thank reviewers and editor for their advice., ANR-10-LABX-0025,CEBA,CEnter of the study of Biodiversity in Amazonia(2010), CCSD, Accord Elsevier, Laboratoires d'excellence - CEnter of the study of Biodiversity in Amazonia - - CEBA2010 - ANR-10-LABX-0025 - LABX - VALID, and Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées (IRBA)

Subjects
Male, Genotyping Techniques, genotype, Gastroenterology, Group B, 0302 clinical medicine, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Genotype, Leishmaniavirus, Leishmania guyanensis, Phylogeny, LRV1, [SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology, [SDV.MHEP.ME] Life Sciences [q-bio]/Human health and pathology/Emerging diseases, [SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseases, 0303 health sciences, biology, General Medicine, French Guiana, [SDV.MHEP.CSC] Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, 3. Good health, Infectious Diseases, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Female, [SDV.MP.PAR] Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology, medicine.drug, Adult, Microbiology (medical), medicine.medical_specialty, 030231 tropical medicine, Leishmaniasis, Cutaneous, Virus, Young Adult, 03 medical and health sciences, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, pentamidine, Internal medicine, medicine, Humans, [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology, Retrospective Studies, treatment failure, 030304 developmental biology, Sequence Analysis, RNA, business.industry, Genetic Variation, Leishmaniasis, medicine.disease, Leishmania, biology.organism_classification, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, business, Pentamidine
Abstract

Objectives The outcome of American tegumentary leishmaniasis (ATL) may depend on the presence of the Leishmania RNA virus (LRV). This virus may be involved in treatment failure. We aimed to determine whether genetic clusters of LRV1 are involved in this therapeutic outcome. Methods The presence of LRV1 was assessed in 129 Leishmania guyanensis isolates from patients treated with pentamidine in French Guiana. Among the 115 (89%) isolates found to carry LRV1, 96 were successfully genotyped. Patient clinical data were linked to the LRV data. Results The rate of treatment failure for LRV1-positive isolates was 37% (15/41) versus 40% (2/5) among LRV1-negative isolates (p 0.88). Concerning LRV1 genotypes, two predominant LRV1 groups emerged, groups A (23% (22/96)) and B (70% (67/96)). The treatment failure rate was 37% (3/8) for group A and 45% (9/20) for group B (p 0.31). Discussion Neither the presence nor genotype of LRV1 in patients with L. guyanensis seemed to correlate with pentamidine treatment failure.

Published
2021
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3. Outbreaks of toxoplasmosis in a captive breeding colony of squirrel monkeys

Author

Stéphane Simon, Daniel Ajzenberg, Magalie Demar, Bernard Carme, Benoit de Thoisy, Marie-Laure Dardé, Bertrand Maubert, Laboratoire Hospitalo-Universitaire de Parasitologie-Mycologie, Université des Antilles (UA)-Coordination Régionale de la lutte contre le Virus de L'Immunodéficience Humaine (COREVIH)-Centre Hospitalier Andrée Rosemon [Cayenne, Guyane Française], Université de Limoges (UNILIM), Neuroépidémiologie Tropicale et Comparée (NETEC), Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST FR CNRS 3503)-Institut d'Epidémiologie Neurologique et de Neurologie Tropicale-Université de Limoges (UNILIM), Centre National de Référence (CNR) Toxoplasmose/Toxoplasma Biological Resource Center (BRC) (CNR Toxoplasmose-Toxoplasma BRC), and CHU Limoges

Subjects
Genotype, 040301 veterinary sciences, 030231 tropical medicine, Breeding, Disease Outbreaks, 0403 veterinary science, 03 medical and health sciences, 0302 clinical medicine, parasitic diseases, Captive breeding, medicine, Animals, Saimiri, Molecular Epidemiology, Virulence, General Veterinary, biology, Monkey Diseases, Squirrel monkey, Outbreak, Toxoplasma gondii, Saimiri sciureus, 04 agricultural and veterinary sciences, General Medicine, biology.organism_classification, medicine.disease, Virology, Toxoplasmosis, 3. Good health, Toxoplasmosis, Animal, Parasitology, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, Toxoplasma
Abstract

International audience; Toxoplasma gondii is highly virulent in New World monkeys, but despite numerous outbreaks observed in captive populations there are few reports of molecular characterization of strains. In this article, we describe two outbreaks of toxoplasmosis that occurred in 2001 and 2006 in an outdoor captive breeding colony of squirrel monkeys (Saimiri sciureus) kept by the Institut Pasteur in French Guiana. A microsatellite DNA analysis of the biological samples collected in the 2001 and 2006 outbreaks showed that two different Toxoplasma strains were involved. The 2001 strain exhibited a type II genotype whereas the 2006 strain showed a combination of type I, type III and atypical alleles. Infection could be related to oocysts contaminating water or food, or to ingestion of rats by monkeys. In 2006, a second episode was observed 3 weeks after the first, and was believed to be related to direct contamination by tachyzoites of bronchopulmonary origin from dying monkeys of the first event. During both outbreaks, a total of 50 monkeys died and none recovered spontaneously, confirming the virulence of both type II and non-type II Toxoplasma strains in New World monkeys.

Published
2009
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4. PADS 2-06 - Paludisme et orpaillage en Guyane : une situation alarmante mais hétérogène

Author

L. Mutricy, F. Djossou, Helene Hiwat, Florine Corlin, Denis Blanchet, Lise Musset, Mathieu Nacher, Maylis Douine, Stéphane Pelleau, M. Demar, Centre d'Investigation Clinique Antilles-Guyane (CIC - Antilles Guyane), Université des Antilles et de la Guyane (UAG)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pointe-à-Pitre/Abymes [Guadeloupe] -CHU de Fort de France-Centre Hospitalier Andrée Rosemon [Cayenne, Guyane Française], Ecosystemes Amazoniens et Pathologie Tropicale (EPat), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Guyane (UG), Centre National de Référence du Paludisme [Cayenne, Guyane française] (CNR), Institut Pasteur de la Guyane, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Centre Hospitalier Andrée Rosemon [Cayenne, Guyane Française], Laboratoire Hospitalo-Universitaire de Parasitologie-Mycologie, Coordination Régionale de la lutte contre le Virus de L'Immunodéficience Humaine (COREVIH)-Centre Hospitalier Andrée Rosemon [Cayenne, Guyane Française]-Université des Antilles (UA), Unité des Maladies Infectieuses et Tropicales (UMIT), Malaria Program, Paramaribo, Suriname, Ministry of Health [Mozambique], and ADENIS, ANTOINE

Subjects
Infectious Diseases, [SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie
Abstract

National audience; Introduction Le paludisme est en nette diminution en Guyane actuellement. Des foyers de transmission persistent en population autochtone, mais cette pathologie touche aujourd’hui majoritairement les orpailleurs clandestins travaillant en Guyane. Cette étude avait pour objectifs d’évaluer le portage de Plasmodium par bassin d’orpaillage et les connaissances, attitudes et pratiques(CAP) des orpailleurs clandestins vis-à-vis du paludisme.

Published
2016
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