25 results on '"Kiyoshi Yoshino"'
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2. Potential pathogenetic link between angiomyofibroblastoma and superficial myofibroblastoma in the female lower genital tract based on a novel MTG1-CYP2E1 fusion
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Kiyoshi Yoshino, Ryuji Iwamura, Aya Nawata, Eisuke Shiba, Chisachi Kubo, Ryosuke Tajiri, Masanori Hisaoka, and Hiroshi Harada
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Adult ,Pathology ,medicine.medical_specialty ,Angiomyofibroblastoma ,Stromal cell ,Genital Neoplasms, Female ,Biology ,Angiofibroma ,GTP Phosphohydrolases ,Pathology and Forensic Medicine ,Neoplasms, Muscle Tissue ,Young Adult ,Biomarkers, Tumor ,medicine ,Humans ,Genetic Predisposition to Disease ,RNA-Seq ,Progenitor cell ,Reverse Transcriptase Polymerase Chain Reaction ,Mesenchymal stem cell ,Cytochrome P-450 CYP2E1 ,Middle Aged ,Immunohistochemistry ,Phenotype ,Fusion transcript ,Female ,Gene Fusion ,Angiomyxoma ,Myofibroblastoma - Abstract
Angiomyofibroblastoma and superficial myofibroblastoma are distinctive benign mesenchymal tumors occurring in the female lower genital tract. Despite their significant overlapping clinicopathologic features, including the presence of bland-looking spindle or oval cells with myofibroblastic or myoid differentiation, the tumors have been regarded as separate entities. Although subepithelial, hormone-sensitive mesenchymal cells of the female lower genital tract are considered as their potential common progenitor cells, their potential kinship or pathogenetic similarities remain elusive. Based on the identification of a novel RNA sequencing-based MTG1-CYP2E1 fusion transcript in an angiomyofibroblastoma index case, we investigated an additional ten samples of the tumor and its site-specific histological mimics, including eight superficial myofibroblastomas, four deep angiomyxomas, four cellular angiofibromas, three fibroepithelial stromal polyps, and eight non-site-specific mesenchymal tumors occurring in the female lower genital tract. Using reverse transcription-polymerase chain reaction, we showed that the MTG1-CYP2E1 fusion transcripts were consistently detectable in angiomyofibroblastomas (5/5, 100%) and often in superficial myofibroblastomas (3/5, 60%) but were not detected in the other examined site-specific or non-site-specific mesenchymal tumors. Our immunohistochemical experiments showed that CYP2E1, an isoenzyme belonging to the cytochrome P450 superfamily, exhibited increased positivity in tumors with MTG1-CYP2E1 than was observed in fusion-negative tumors (RR = 6.56, p = 0.001). The results of our study provide further evidence supporting the assertion that angiomyofibroblastoma and superficial myofibroblastoma represent phenotypic variants of site-specific mesenchymal tumors and share a common oncogenic mechanism.
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- 2021
3. Pilot study of repetitive transcranial magnetic stimulation in patients with chemotherapy-induced peripheral neuropathy
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Kiyoshi Yoshino, Tomoo Mano, Toshio Shimokawa, Takeshi Shimizu, Koichi Hosomi, Seung Jin Kim, Yuko Goto, Haruhiko Kishima, and Youichi Saitoh
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Adult ,Male ,Visual Analog Scale ,Visual analogue scale ,medicine.medical_treatment ,Pilot Projects ,03 medical and health sciences ,0302 clinical medicine ,Physiology (medical) ,medicine ,Humans ,Pain Management ,Paresthesia ,Pain Measurement ,Dysesthesia ,business.industry ,Motor Cortex ,Common Terminology Criteria for Adverse Events ,General Medicine ,Middle Aged ,medicine.disease ,Transcranial Magnetic Stimulation ,Transcranial magnetic stimulation ,Peripheral neuropathy ,Neurology ,Chemotherapy-induced peripheral neuropathy ,McGill Pain Questionnaire ,030220 oncology & carcinogenesis ,Anesthesia ,Neuropathic pain ,Neuralgia ,Female ,Surgery ,Neurology (clinical) ,medicine.symptom ,business ,030217 neurology & neurosurgery - Abstract
Objective Chemotherapy-induced peripheral neuropathy (CIPN) is one of the intractable long-term side effects of anticancer medications and results in pain and dysesthesia. Repetitive transcranial magnetic stimulation (rTMS) of the primary motor cortex has been demonstrated to provide effective relief for intractable neuropathic pain. The objective of this study was to investigate the effects of rTMS treatment on CIPN in cancer patients. Materials and methods Eleven female patients with breast cancer or gynecologic cancer (mean age 64.8 [standard deviation 7.8]) who had neuropathic pain and/or peripheral sensory neuropathy, with a minimum two grade severity based on the scale of the National Cancer Institutes’ Common Terminology Criteria for Adverse Events (version 4.0) were enrolled. Patients received rTMS (5-Hz; 500 pulses/session; figure-8 coil) on their primary motor cortex corresponding to the target extremity. The intensity of pain and dysesthesia for all extremities was evaluated using a visual analog scale for pain, dysesthesia, and the Japanese version of the short-form McGill Pain Questionnaire 2 (SFMPQ2). Results rTMS for target extremity significantly decreased the visual analog scale of pain and dysesthesia. The intensity of pain measured by the SFMPQ2 was also decreased in the target extremity. Regarding non-target extremities, only dysesthesia significantly decreased as a result of rTMS. No adverse events were observed. Conclusion This is an initial report demonstrating the potential of rTMS for the treatment of CIPN. We suggest rTMS could be potentially beneficial and effective as a treatment for pain and dysesthesia in patients with CIPN.
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- 2020
4. Fertility-sparing trachelectomy for early-stage cervical cancer: A proposal of an ideal candidate
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Tomoaki Ikeda, Kyoko Tanaka, Hiroaki Kobayashi, Hideki Tokunaga, Koji Matsuo, Mikio Mikami, Kenichiro Morishige, Nobuo Yaegashi, Kiyoshi Yoshino, Hiroko Machida, Takashi Iwata, Takayuki Enomoto, Tsuyoshi Saito, Makio Shozu, and Kaoru Okugawa
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Adult ,0301 basic medicine ,medicine.medical_specialty ,Trachelectomy ,medicine.medical_treatment ,Uterine Cervical Neoplasms ,Disease-Free Survival ,Article ,Fertility sparing surgery ,Cohort Studies ,03 medical and health sciences ,0302 clinical medicine ,Obstetrics and gynaecology ,medicine ,Humans ,Neoplasm Invasiveness ,Stage (cooking) ,Neoplasm Staging ,Retrospective Studies ,Cervical cancer ,Hysterectomy ,Ideal (set theory) ,Obstetrics ,business.industry ,Fertility Preservation ,Obstetrics and Gynecology ,Retrospective cohort study ,medicine.disease ,030104 developmental biology ,Oncology ,Lymphatic Metastasis ,030220 oncology & carcinogenesis ,Female ,business - Abstract
OBJECTIVE. To propose an ideal patient candidate with early-stage cervical cancer for undergoing fertility-sparing trachelectomy. METHODS. This nationwide, multicenter, retrospective study was conducted by the Japan Society of Obstetrics and Gynecology involving women aged
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- 2020
5. Histopathological finding of 5 cases of CAOS without genital bleeding
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Ryosuke Tajiri, Eiji Shibata, Yuma Saito, Mao Sekimata, Haruka Akaji, Yasuyuki Kinjo, Emi Kondo, and Kiyoshi Yoshino
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Reproductive Medicine ,Obstetrics and Gynecology ,Developmental Biology - Published
- 2022
6. Measurement of excited singlet oxygen molecule in a vacuum sterilization system, using electric spin resonance (ESR) with a water-soluble polymer thin film
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Satoru Iwamori and Kiyoshi Yoshino
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010302 applied physics ,Chemistry ,General Chemical Engineering ,General Physics and Astronomy ,chemistry.chemical_element ,02 engineering and technology ,General Chemistry ,Sterilization (microbiology) ,021001 nanoscience & nanotechnology ,Photochemistry ,01 natural sciences ,Oxygen ,law.invention ,Magazine ,law ,Excited state ,Reagent ,0103 physical sciences ,Molecule ,Thin film ,0210 nano-technology ,Electron paramagnetic resonance - Abstract
An advanced sterilization system employing active oxygen species (AOS) was investigated. We revealed in a previous paper that, by using active oxygen species generated from oxygen gas by ultraviolet irradiation in the chamber of the sterilization system, the survival curves of Geobacillus stearothermophilus spores (10 6 CFU), a biological indicator (BI), showed exponential reduction, and that this sterilization was attributable to the UV-generated AOS. However, we had little knowledge of what specific types of AOS were primarily responsible for the sterilization effects. Therefore, in this study we investigated the principal sterilization contributors among the AOS generated by ultraviolet irradiation, using the electron spin resonance (ESR) technique, with 2,2,6,6-tetramethyl-4-piperidinol (TEMP) as a spin-label reagent for the excited state of atomic oxygen molecules ( 1 O 2 ), and compared the respective sterilization effects of AOS and oxygen plasma system.
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- 2016
7. Significance of histologic pattern of carcinoma and sarcoma components on survival outcomes of uterine carcinosarcoma
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D. Kadogami, Tanja Pejovic, Toru Sugiyama, Masato Nishimura, Takashi Sasaki, Shinya Matsuzaki, Erin A. Blake, Melissa Moffitt, Tadaaki Nishikawa, Lynda D. Roman, A. Wakatsuki, Takuya Moriya, Tsukasa Baba, Frederick R. Ueland, M. Yoshida, Kiyoshi Yoshino, Munetaka Takekuma, Mian M.K. Shahzad, Kazuaki Suda, H. Yoshida, Merieme Klobocista, Miriam D. Post, Kei Kawana, Tetsuro Oishi, T. Yokoyama, Hiroko Machida, Hiroshi Kajiwara, Esther Elishaev, Ken Yamaguchi, Yasuhiko Shiki, M. Andoh, Mikio Mikami, Yutaka Takazawa, Joseph L. Kelley, Tadashi Kimura, Abby M. Richmond, Tomoyuki Fukagawa, Tadayoshi Nagano, Masanori Yasuda, Y. Hazama, I. Podzielinski, Y. Ikeda, D. D. Im, K. Fujiwara, Norichika Ushioda, Koichiro Shimoya, Muneaki Shimada, Marian S. Johnson, Masako Shida, Sosuke Adachi, Koji Matsuo, Y. Ueda, Stephen H. Bush, Shinya Satoh, Ikuo Konishi, Kohei Omatsu, Takayuki Enomoto, Takahito Miyake, K. Iwasaki, Rouzan G. Karabakhtsian, Yoshiaki Yuba, Malcolm S. Ross, Tadao Takano, Terry K. Morgan, Todd B. Sheridan, Satoshi Takeuchi, Kosei Hasegawa, S. W. Li, Paulette Mhawech-Fauceglia, Mayu Yunokawa, and Ardeshir Hakam
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Adult ,0301 basic medicine ,Oncology ,medicine.medical_specialty ,Heterologous ,Disease-Free Survival ,03 medical and health sciences ,0302 clinical medicine ,Carcinosarcoma ,Internal medicine ,medicine ,Carcinoma ,Humans ,Ifosfamide ,Uterine Neoplasm ,Survival analysis ,Aged ,Neoplasm Staging ,Retrospective Studies ,business.industry ,Sarcoma ,Hematology ,Middle Aged ,medicine.disease ,Survival Analysis ,Chemotherapy regimen ,Treatment Outcome ,030104 developmental biology ,Chemotherapy, Adjuvant ,030220 oncology & carcinogenesis ,Uterine Neoplasms ,Female ,Radiotherapy, Adjuvant ,business ,medicine.drug - Abstract
To examine the effect of the histology of carcinoma and sarcoma components on survival outcome of uterine carcinosarcoma.A multicenter retrospective study was conducted to examine uterine carcinosarcoma cases that underwent primary surgical staging. Archived slides were examined and histologic patterns were grouped based on carcinoma (low-grade versus high-grade) and sarcoma (homologous versus heterologous) components, correlating to clinico-pathological demographics and outcomes.Among 1192 cases identified, 906 cases were evaluated for histologic patterns (carcinoma/sarcoma) with high-grade/homologous (40.8%) being the most common type followed by high-grade/heterologous (30.9%), low-grade/homologous (18.0%), and low-grade/heterologous (10.3%). On multivariate analysis, high-grade/heterologous (5-year rate, 34.0%, P = 0.024) and high-grade/homologous (45.8%, P = 0.017) but not low-grade/heterologous (50.6%, P = 0.089) were independently associated with decreased progression-free survival (PFS) compared with low-grade/homologous (60.3%). In addition, older age, residual disease at surgery, large tumor, sarcoma dominance, deep myometrial invasion, lymphovascular space invasion, and advanced-stage disease were independently associated with decreased PFS (all, P0.01). Both postoperative chemotherapy (5-year rates, 48.6% versus 39.0%, P0.001) and radiotherapy (50.1% versus 44.1%, P = 0.007) were significantly associated with improved PFS in univariate analysis. However, on multivariate analysis, only postoperative chemotherapy remained an independent predictor for improved PFS [hazard ratio (HR) 0.34, 95% confidence interval (CI) 0.27-0.43, P0.001]. On univariate analysis, significant treatment benefits for PFS were seen with ifosfamide for low-grade carcinoma (82.0% versus 49.8%, P = 0.001), platinum for high-grade carcinoma (46.9% versus 32.4%, P = 0.034) and homologous sarcoma (53.1% versus 38.2%, P = 0.017), and anthracycline for heterologous sarcoma (66.2% versus 39.3%, P = 0.005). Conversely, platinum, taxane, and anthracycline for low-grade carcinoma, and anthracycline for homologous sarcoma had no effect on PFS compared with non-chemotherapy group (all, P0.05). On multivariate analysis, ifosfamide for low-grade/homologous (HR 0.21, 95% CI 0.07-0.63, P = 0.005), platinum for high-grade/homologous (HR 0.36, 95% CI 0.22-0.60, P0.001), and anthracycline for high-grade/heterologous (HR 0.30, 95% CI 0.14-0.62, P = 0.001) remained independent predictors for improved PFS. Analyses of 1096 metastatic sites showed that carcinoma components tended to spread lymphatically, while sarcoma components tended to spread loco-regionally (P0.001).Characterization of histologic pattern provides valuable information in the management of uterine carcinosarcoma.
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- 2016
8. A Case of Extragonadal Teratoma in the Pouch of Douglas and Literature Review
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Eiji Kobayashi, Shinya Matsuzaki, Tadashi Kimura, Kiyoshi Yoshino, Eiichi Morii, and Mamoru Kakuda
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Adult ,endocrine system ,Pathology ,medicine.medical_specialty ,Extragonadal ,endocrine system diseases ,Ovary ,Rare Diseases ,medicine ,Humans ,Dermoid Cyst ,Ovarian Neoplasms ,business.industry ,Abdominal Wall ,Teratoma ,Obstetrics and Gynecology ,Myoma ,Neoplasms, Germ Cell and Embryonal ,Greater omentum ,medicine.disease ,Surgery ,medicine.anatomical_structure ,Dermoid cyst ,Female ,Laparoscopy ,Pouch ,business ,Omentum ,Fallopian tube - Abstract
Mature cystic teratoma is a germ cell tumor of the ovaries and is often observed in clinical practice. However, extragonadal teratomas are rare tumors and have been reported outside the ovaries, (e.g., in the greater omentum). The mechanism underlying the development of extragonadal teratomas remains unknown. We encountered a case of extragonadal teratoma in the pouch of Douglas that appeared to be a parasitic dermoid cyst. From our experience and the literature review, we discuss the potential mechanism leading to the development of extragonadal teratomas. A 41-year-old nonpregnant woman was referred to our department due to myoma and anemia. A 4-cm asymptomatic mass in the pouch of Douglas was observed, and the patient was diagnosed with ovarian mature cystic teratoma. She underwent laparoscopic surgery, and intraoperative findings revealed that the fallopian tube was injured and torn, and a residual small ovary was observed in the left side of the ovary. A tumor measuring approximately 4 cm observed in the pouch of Douglas was extracted without rupturing. The tumor was diagnosed as a parasitic dermoid cyst by macroscopic and histopathological findings. Auto-amputation could be the underlying mechanism that leads to an isolated parasitic dermoid cyst in the pouch of Douglas.
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- 2015
9. Venous thromboembolism, interleukin-6 and survival outcomes in patients with advanced ovarian clear cell carcinoma
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Noriomi Matsumura, Kosuke Hiramatsu, Takayuki Enomoto, Kiyoshi Yoshino, Koji Matsuo, Ryusuke Murakami, Kosei Hasegawa, Akiko Miyara, Hani Gabra, Rebecca L. Stone, Christina Fotopoulou, Takeshi Hisamatsu, Yuji Ikeda, Ikuo Konishi, Rebecca A. Previs, Anil K. Sood, Lynda D. Roman, Keiichi Fujiwara, and Jean M. Hansen
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Oncology ,Cancer Research ,Time Factors ,MICROENVIRONMENT ,Kaplan-Meier Estimate ,Japan ,Risk Factors ,Ovarian carcinoma ,Clear cell carcinoma ,Ovarian Neoplasms ,Incidence ,Hazard ratio ,Venous Thromboembolism ,Middle Aged ,CANCER ,Up-Regulation ,Serous fluid ,Tumor Markers, Biological ,Disease Progression ,Female ,Life Sciences & Biomedicine ,EPITHELIAL OVARIAN ,EXPRESSION ,Adult ,medicine.medical_specialty ,Article ,Disease-Free Survival ,Ovarian cancer ,Internal medicine ,Biomarkers, Tumor ,medicine ,Carcinoma ,Humans ,Oncology & Carcinogenesis ,Aged ,Neoplasm Staging ,Proportional Hazards Models ,Retrospective Studies ,IL-6 ,Science & Technology ,Survival outcome ,Interleukin-6 ,business.industry ,Proportional hazards model ,Retrospective cohort study ,medicine.disease ,United States ,CYTOKINE ,THROMBOSIS ,Multivariate Analysis ,Neoplasm Recurrence, Local ,Neoplasms, Cystic, Mucinous, and Serous ,business ,2-TIER SYSTEM ,1112 Oncology And Carcinogenesis - Abstract
Background: We compared survival outcomes and risk of venous thromboembolism (VTE) among patients with advanced and early-stage ovarian clear cell carcinoma (OCCC) and serous ovarian carcinoma (SOC), as well as potential links with interleukin-6 (IL-6) levels. Methods: A multicenter case-control study was conducted in 370 patients with OCCC and 938 with SOC. In a subset of 200 cases, pretreatment plasma IL-6 levels were examined. Findings: Patients with advanced OCCC had the highest 2-year cumulative VTE rates (advanced OCCC 43.1%, advanced SOC 16.2%, early-stage OCCC 11.9% and early-stage SOC 6.4%, P < 0.0001) and the highest median levels of IL-6 (advanced OCCC 17.8 pg/mL, advanced SOC 9.0 pg/mL, early-stage OCCC 4.2 pg/mL and early-stage SOC 5.0 pg/mL, P = 0.006). Advanced OCCC (hazard ratio [HR] 3.38, P < 0.0001), thrombocytosis (HR 1.42, P = 0.032) and elevated IL-6 (HR 8.90, P = 0.046) were independent predictors of VTE. In multivariate analysis, patients with advanced OCCC had significantly poorer 5-year progression-free and overall survival rates than those with advanced SOC (P < 0.01), and thrombocytosis was an independent predictor of decreased survival outcomes (P < 0.01). Elevated IL-6 levels led to poorer 2-year progression-free survival rates in patients with OCCC (50% versus 87.5%, HR 4.89, P = 0.016) than in those with SOC (24.9% versus 40.8%, HR 1.40, P = 0.07). Interpretation: Advanced OCCC is associated with an increased incidence of VTE and decreased survival outcomes, which has major implications for clinical management of OCCC.
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- 2015
10. Survival outcome of stage I ovarian clear cell carcinoma with lympho-vascular space invasion
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Ikuo Konishi, Anil K. Sood, Kosuke Hiramatsu, Todd B. Sheridan, Kiyoshi Yoshino, Takayuki Enomoto, Ryusuke Murakami, Christina Fotopoulou, Sosuke Adachi, Koji Matsuo, Kosei Hasegawa, Lynda D. Roman, Keiichi Fujiwara, Masato Nishimura, Noriomi Matsumura, Takuhei Yokoyama, and Yuji Ikeda
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Oncology ,medicine.medical_specialty ,medicine.medical_treatment ,Carcinoma, Ovarian Epithelial ,Risk Factors ,Internal medicine ,medicine ,Humans ,Neoplasms, Glandular and Epithelial ,Stage (cooking) ,Survival analysis ,Lymphatic Vessels ,Neoplasm Staging ,Ovarian Neoplasms ,Univariate analysis ,business.industry ,Hazard ratio ,Obstetrics and Gynecology ,Middle Aged ,medicine.disease ,Survival Analysis ,Regimen ,Treatment Outcome ,Lymphatic Metastasis ,Clear cell carcinoma ,Lymph Node Excision ,Female ,Lymphadenectomy ,Lymph Nodes ,Ovarian cancer ,business ,Adenocarcinoma, Clear Cell - Abstract
Background The clinical impact of lympho-vascular space invasion (LVSI) in early-stage ovarian clear cell carcinoma (OCCC) is not well understood. Given the distinct tumor biology and survival patterns of OCCC, the significance of LVSI on survival outcome and treatment response was examined in OCCC. Methods A multicenter study was conducted to examine stage IA–IC3 OCCC cases that underwent primary surgical staging including lymphadenectomy. LVSI status was determined from archived histopathology slides, correlated with clinico-pathological results, chemotherapy patterns, and survival outcomes. Results LVSI was observed in 47 (20.3%) among 232 cases. In univariate analysis, LVSI was associated with older age ( p =0.042), large tumor size ( p =0.048), and stage IC ( p =0.035). In survival analysis, LVSI was associated with decreased disease-free survival (DFS, 5-year rate, 70.6% versus 92.1%, p =0.0004) and overall survival (OS, 78.8% versus 93.3%, p =0.008) on univariate analysis. After controlling for age, tumor size, stage, and chemotherapy use, LVSI remained an independent prognostic factor for decreased survival outcomes (DFS, hazard ratio [HR] 4.35, 95% confidence interval [CI] 1.73–10.9, p =0.002; and OS, HR 4.73, 95%CI 1.60–14.0, p =0.015). Among 210 cases who received postoperative chemotherapy, while regimen type did not impact survival outcome regardless of LVSI status (DFS, p =0.63), the number of administered cycles showed a survival benefit towards ≥6cycles for patients with LVSI-positive tumors (DFS, p =0.009; and OS, p =0.016). Conclusion LVSI is an important marker to predict survival outcome of stage I OCCC. Regardless of chemotherapy type, patients with stage I OCCC showing LVSI may benefit from receiving postoperative chemotherapy.
- Published
- 2015
11. A phase II study of combination chemotherapy using docetaxel and irinotecan for TC-refractory or TC-resistant ovarian carcinomas (GOGO-OV2 study) and for primary clear or mucinous ovarian carcinomas (GOGO-OV3 Study)
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Masaaki Nagamatsu, Kiyoshi Yoshino, Takashi Miyatake, Masami Fujita, Yutaka Ueda, Tadashi Kimura, Yukihiro Nishio, Tateki Tsutsui, Masato Yamasaki, and Takayuki Enomoto
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Adult ,Oncology ,medicine.medical_specialty ,Paclitaxel ,medicine.medical_treatment ,Antineoplastic Agents ,Docetaxel ,Irinotecan ,Disease-Free Survival ,Carboplatin ,Japan ,Internal medicine ,Ovarian carcinoma ,Antineoplastic Combined Chemotherapy Protocols ,medicine ,Humans ,Prospective Studies ,Progression-free survival ,neoplasms ,Aged ,Ovarian Neoplasms ,Chemotherapy ,business.industry ,Obstetrics and Gynecology ,Combination chemotherapy ,Middle Aged ,medicine.disease ,Adenocarcinoma, Mucinous ,Regimen ,Reproductive Medicine ,Drug Resistance, Neoplasm ,Camptothecin ,Female ,Taxoids ,Ovarian cancer ,business ,Adenocarcinoma, Clear Cell ,medicine.drug - Abstract
Objective To analyze the efficacy and safety of combination chemotherapy of docetaxel and irinotecan for paclitaxel and carboplatin (TC) -refractory or -resistant ovarian carcinomas and for first treatment of primary clear cell and mucinous ovarian carcinomas. Study design Between 2002 and 2009, we conducted a prospective Phase II study of the efficacy and safety of combination chemotherapy using docetaxel and irinotecan in 62 patients with TC-refractory or -resistant ovarian carcinoma cases (GOGO-OV2) and 15 patients with primary clear cell and mucinous ovarian carcinoma cases (GOGO-OV3). The dose of docetaxel and irinotecan was determined during our previous Phase I study. Results A docetaxel plus irinotecan regimen provided a 53% response rate, 6 months progression-free survival (PFS), and 12 months overall survival (OS) for primary clear cell and mucinous ovarian carcinomas (similar to TC therapy). The differences of anti-tumor and survival effects between refractory and resistant cases were not statistically significant. The regimen also provided a 15% response rate, 5 months PFS, and 15 months OS for TC-refractory or TC-resistant cases, when used as a second-line chemotherapy. These data are similar to previous reports, however, our study provides the first data exclusively for the cases refractory or resistant to a gold standard TC therapy as a second-line chemotherapy. The regimen was demonstrated to be well tolerable. Conclusion Combination chemotherapy of docetaxel and irinotecan may be a useful option to treat TC-refractory/resistant cases and primary clear cell and mucinous adenocarcinoma cases of ovarian carcinoma.
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- 2013
12. Monitoring of sterilization in an oxygen plasma apparatus, employing a quartz crystal microbalance (QCM) method
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Shinobu Kinoshita, Tatsuyuki Iwasaki, Hiroyuki Matsumoto, Kazutoshi Noda, Kiyoshi Yoshino, and Satoru Iwamori
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Chemistry ,Scanning electron microscope ,Oxygen plasma ,Analytical chemistry ,Atomic oxygen ,Fluorocarbon ,Quartz crystal microbalance ,Spectrum analysis ,Sterilization (microbiology) ,Thin film ,Condensed Matter Physics ,Instrumentation ,Surfaces, Coatings and Films - Abstract
With the aim of constructing a system for monitoring sterilization under atomic oxygen, we investigated the applicability of the quartz crystal microbalance (QCM) system, to atomic oxygen generated in a cold oxygen plasma apparatus. We have exposed microorganisms, such as Bacillus subtilis and Aspergillus niger , examined the effects of such exposure, and observed the inactivation properties of these microorganisms. We have confirmed that there is a positive correlation between atomic oxygen exposure and inactivation of microorganisms, quantified by the QCM method using fluorocarbon thin films, and have investigated the relevant inactivation mechanism through scanning electron microscope (SEM) observation and spectrum analysis.
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- 2013
13. Conization using the Shimodaira-Taniguchi procedure for adenocarcinoma in situ of the uterine cervix
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Takayuki Enomoto, Kiyoshi Yoshino, Masami Fujita, Yutaka Ueda, Eiichi Morii, Tadashi Kimura, and Kosuke Hiramatsu
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medicine.medical_specialty ,Hysterectomy ,business.industry ,medicine.medical_treatment ,Medical record ,Adenocarcinoma in situ ,Obstetrics and Gynecology ,Retrospective cohort study ,University hospital ,Surgery ,Conservative treatment ,Uterine cervix ,Reproductive Medicine ,medicine ,Resection margin ,business - Abstract
Objective The Shimodaira-Taniguchi conization procedure addresses the disadvantages of the loop electrosurgical excision procedure (LEEP) by using a high frequency current and a triangular probe with a linear excision electrode to extract the tissue as a single informative specimen, without incurring accompanying thermal trauma. The aim of the present study was to analyze the surgical efficacy of the Shimodaira-Taniguchi conization procedure for adenocarcinoma in situ (AIS) of the uterine cervix. Study design At the Osaka University Hospital, conization using the Shimodaira-Taniguchi procedure has long been routinely performed for AIS. Medical records of patients during the period from 2005 to 2011, whose post-conization diagnosis was AIS, were retrospectively analyzed. A literature review was conducted of the PubMed database to clarify the surgical outcome efficacy of the Shimodaira-Taniguchi procedure compared to other procedures. Results During the study period, a post-conization diagnosis of AIS was made in 10 patients. A positive resection margin was detected in 4 of the 10 cases (40%), and residual disease was observed in 3 cases (30%). A review of the relevant literature indicates that the rate of positive margin and residual disease by the Shimodaira-Taniguchi procedure, including our cases, was not significantly different from the cold knife, LEEP or laser procedures ( p =0.32, 0.99, and 0.40, respectively, for positive margin, and p =0.76, 0.94, and 0.063, respectively, for residual disease). Conclusion AIS was demonstrated to be efficaciously treated, with a low risk of residual disease, by the Shimodaira-Taniguchi conization procedure. Further study is still needed to establish a standard of conservative treatment for AIS.
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- 2013
14. Active oxygen sensors used a quartz crystal microbalance (QCM) with sputter-coated and spin-coated poly(tetrafluoroethylene) thin films
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Hiroyuki Matsumoto, Kazutoshi Noda, Kiyoshi Yoshino, Itsuo Nishiyama, and Satoru Iwamori
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Spin coating ,Materials science ,genetic structures ,Layer by layer ,Metals and Alloys ,Quartz crystal microbalance ,Condensed Matter Physics ,eye diseases ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,chemistry.chemical_compound ,chemistry ,Chemical engineering ,Sputtering ,Physical vapor deposition ,Materials Chemistry ,Organic chemistry ,Tetrafluoroethylene ,sense organs ,Fluorocarbon ,Electrical and Electronic Engineering ,Thin film ,Instrumentation - Abstract
Organic thin films prepared by physical vapor deposition (PVD) have complex structures compared to the pristine materials. Therefore, these thin films have potentials to show different surface properties. Poly(tetrafluoroethylene) (PTFE) is one of the fluorocarbon polymers, and fluorocarbon thin films deposited by an r.f. sputtering with a PTFE target and spin coating with PTFE dispersion on a quartz crystal were investigated to determine their suitability for application in active oxygen sensors. Active oxygen generated under ultraviolet irradiation was successfully detected from frequency shifts using the QCM electrode coated with the fluorocarbon thin films. The sensitivity of the thin film prepared by the r.f. sputtering for active oxygen was higher than that of the thin film prepared by the spin coating. It is known that molecular structures of the fluorocarbon thin film prepared by the r.f. sputtering with a PTFE target were quite different from pristine PTFE. It is considered that this is one of the reasons why the sensitivity of the sputtered thin film for active oxygen was higher than that of the thin film prepared by the spin coating.
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- 2012
15. Postoperative concurrent nedaplatin-based chemoradiotherapy improves survival in early-stage cervical cancer patients with adverse risk factors
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Yasuo Yoshioka, Seiji Mabuchi, Tadashi Kimura, Fumiaki Isohashi, Kiyoshi Yoshino, Toshiya Yamamoto, Takehiro Inoue, Takashi Takeda, Ken-ichirou Morishige, and Takayuki Enomoto
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Adult ,Oncology ,medicine.medical_specialty ,Organoplatinum Compounds ,medicine.medical_treatment ,Uterine Cervical Neoplasms ,Antineoplastic Agents ,chemistry.chemical_compound ,Risk Factors ,Internal medicine ,medicine ,Adjuvant therapy ,Humans ,Nedaplatin ,External beam radiotherapy ,Progression-free survival ,Radical surgery ,Neoplasm Staging ,Retrospective Studies ,Cervical cancer ,business.industry ,Obstetrics and Gynecology ,Middle Aged ,Prognosis ,medicine.disease ,Radiation therapy ,Treatment Outcome ,chemistry ,Chemotherapy, Adjuvant ,Female ,Radiotherapy, Adjuvant ,business ,Chemoradiotherapy - Abstract
Objectives The aim of this study was to evaluate the efficacy of postoperative nedaplatin-based concurrent chemoradiotherapy (CCRT) in patients with FIGO stage IA2–IIB cervical cancer with adverse risk factors. Methods We retrospectively reviewed the medical records of 183 patients with early-stage cervical cancer who had undergone radical surgery between April 1997 and March 2006. Of these, 68 patients displayed high-risk prognostic factors such as positive pelvic lymph nodes, parametrial involvement, or a positive surgical margin. Fifty-seven patients demonstrated intermediate-risk prognostic factors including deep stromal invasion, capillary lymphatic space involvement, or large tumor diameter. These patients were treated postoperatively with CCRT or radiotherapy alone (RT). Fifty-eight patients showed no risk factors and, therefore, received no adjuvant therapy after surgery. The 3-year recurrence rate, progression free survival (PFS), and overall survival (OS) were compared between the treatment groups. Results CCRT was significantly superior to RT alone with regard to recurrence rate, PFS, and OS in patients that displayed high-risk and intermediate-risk prognostic factors. The frequencies of acute grade 3–4 toxicities were significantly higher in patients treated with CCRT than in those treated with RT alone. However, no statistically significant difference was observed with regard to severe late toxicities. Conclusions Postoperative nedaplatin-based CCRT was safely performed and improved the prognosis of FIGO stage IA2–IIB cervical cancer patients displaying high-risk or intermediate-risk prognostic factors. This treatment can be considered as an alternative to cisplatin-based chemoradiotherapy in this patient population.
- Published
- 2009
16. PIK3CA gene mutations and amplifications in uterine cancers, identified by methods that avoid confounding by PIK3CA pseudogene sequences
- Author
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Hiromi Ugaki, Tadashi Kimura, Masami Fujita, Ayako Kim, Takayuki Enomoto, Tomomi Takata, Kazuko Fujiwara, Kiyoshi Yoshino, Takashi Miyatake, and Takahito Miyake
- Subjects
Cancer Research ,Pseudogene ,Molecular Sequence Data ,Gene Dosage ,Gene mutation ,Biology ,medicine.disease_cause ,Gene dosage ,Phosphatidylinositol 3-Kinases ,Exon ,Uterine cancer ,Cell Line, Tumor ,Gene duplication ,medicine ,Humans ,neoplasms ,Gene ,Mutation ,Gene Amplification ,medicine.disease ,Endometrial Neoplasms ,Oncology ,Uterine Neoplasms ,Cancer research ,Female ,Pseudogenes - Abstract
PIK3CA codes for a Class IA p110-alpha catalytic subunit of the PI3Ks (phosphatidylinositol 3-kinases) that regulate various signaling pathways important for neoplasia, including cell proliferation, motility, adhesion, and survival. Pro-oncogenic mutations in exons 9 and 20 of the PIK3CA gene have been frequently observed in numerous types of human malignancies. Amplification of the PIK3CA gene has been reported in uterine cervical cancers. In this study, we have done in depth analysis of uterine cervical and endometrial cancers for PIK3CA gene mutations and amplifications. In uterine cervical cancers, PIK3CA mutations were found in 3 of 22 cases (14%), all of them in exon 9. In endometrial cancers, a similar incidence of mutations was found, in 3 of 29 cases (10%), however they were all within exon 20. Amplification of the PIK3CA gene was also detected in 2 out of 22 (9%) cervical cancers and 3 out of 29 (10%) endometrial cancers. In this study, we were unable to find a clear association between PIK3CA mutations and gene amplifications, nor with tumor histological subtypes or staging. Mutations and amplifications of the PIK3CA gene are relatively infrequent in human cervical and endometrial cancers; however, PIK3CA gene alteration may still play a role in some subset of uterine cancers.
- Published
- 2008
17. Development of 3 DOF Ultrasonic Vibration Tool for Elliptical Vibration Cutting of Sculptured Surfaces
- Author
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Eiji Shamoto, H. Inagaki, Kiyoshi Yoshino, Y. Hori, E. Tsuchiya, and Norikazu Suzuki
- Subjects
Surface (mathematics) ,Engineering ,business.product_category ,Cutting tool ,business.industry ,Mechanical Engineering ,Acoustics ,Structural engineering ,Industrial and Manufacturing Engineering ,Machine tool ,Vibration ,Machining ,Milling cutter ,Die (manufacturing) ,Ultrasonic sensor ,business - Abstract
A new machining method is proposed to obtain sculptured mirror surfaces by applying elliptical vibration cutting. The tool is vibrated elliptically unlike rotated end mills and fed along the sculptured surface in the proposed method. A 3 DOF ultrasonic vibration tool is developed, which can generate an arbitrary ultrasonic elliptical vibration in the 3D space so that it is suitable to machine the 3D sculptured surfaces. A precision machine tool is also developed, and the proposed method is successfully applied to mirror surface machining of hardened die steel.
- Published
- 2005
18. Characterization of Glis2, a Novel Gene Encoding a Gli-related, Krüppel-like Transcription Factor with Transactivation and Repressor Functions
- Author
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Anton M. Jetten, Kiyoshi Yoshino, Yong-Sik Kim, Alan Perantoni, Shogo Kurebayashi, Feng Zhang, and Gen Nakanishi
- Subjects
Zinc finger ,Regulation of gene expression ,Krüppel ,YY1 ,GATAD2B ,GLIS2 Gene ,Repressor ,Cell Biology ,Biology ,Molecular Biology ,Biochemistry ,Molecular biology ,Regulator gene - Abstract
In this study, we describe the characterization of a gene encoding a novel Kruppel-like protein, named Glis2. Glis2 encodes a relatively proline-rich, basic 55.8-kDa protein. Its five tandem Cys2-His2 zinc finger motifs exhibit the highest homology to those of members of the Gli and Zic subfamilies of Kruppel-like proteins. Confocal microscopic analysis demonstrated that Glis2 localizes to the nucleus. Analysis of the genomic structure of the Glis2 gene showed that it is composed of 6 exons separated by 5 introns spanning a genomic region of more than 7.5 kb. Fluorescence in situ hybridization mapped the mouse Glis2 gene to chromosome 16A3-B1. Northern blot analysis showed that the Glis2 gene encodes a 3.8-kb transcript that is most abundant in adult mouse kidney. By in situ hybridization, expression was localized to somites and neural tube, and during metanephric development predominantly to the ureteric bud, precursor of the collecting duct, and inductor of nephronic tubule formation. One-hybrid analysis using Glis2 deletion mutants identified a novel activation function (AF) at the N terminus. The activation of transcription through this AF domain was totally suppressed by two repressor functions just downstream from the AF. One of the repressor functions is contained within the first zinc finger motif. The level of transcriptional activation and repression varied with the cell line tested, which might be due to differences in cell type-specific expression of co-activators and co-repressors. Our results suggest that Glis2 behaves as a bifunctional transcriptional regulator. Both the activation and repressor functions may play an important role in the regulation of gene expression during embryonic development.
- Published
- 2002
19. Secreted Frizzled-related proteins can regulate metanephric development
- Author
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Vasiliki Anest, Kathleen G. Higinbotham, Kiyoshi Yoshino, Aykut Üren, Jeffrey S. Rubin, Sergei Y. Plisov, and Alan O. Perantoni
- Subjects
Embryology ,Frizzled ,Time Factors ,Kidney development ,Nephron ,Kidney ,Epithelium ,Mesoderm ,Mice ,Wnt4 Protein ,Cells, Cultured ,In Situ Hybridization ,education.field_of_study ,Intracellular Signaling Peptides and Proteins ,Wnt signaling pathway ,Gene Expression Regulation, Developmental ,Cell Differentiation ,Cadherins ,Immunohistochemistry ,Recombinant Proteins ,Cell biology ,Kidney Tubules ,medicine.anatomical_structure ,Protein Binding ,Signal Transduction ,Mesenchyme ,Immunoblotting ,LIM-Homeodomain Proteins ,Population ,Enzyme-Linked Immunosorbent Assay ,Biology ,Proto-Oncogene Proteins ,Metanephros ,medicine ,Animals ,education ,Cell Nucleus ,Homeodomain Proteins ,Dose-Response Relationship, Drug ,Membrane Proteins ,Proteins ,DNA ,Nephrons ,Frizzled Receptors ,Rats ,Wnt Proteins ,Protein Biosynthesis ,Transcription Factors ,Developmental Biology - Abstract
Wnt-4 signaling plays a critical role in kidney development and is associated with the epithelial conversion of the metanephric mesenchyme. Furthermore, secreted Frizzled-related proteins (sFRPs) that can bind Wnts are normally expressed in the developing metanephros, and function in other systems as modulators of Wnt signaling. sfrp-1 is distributed throughout the medullary and cortical stroma in the metanephros, but is absent from condensed mesenchyme and primitive tubular epithelia of the developing nephron where wnt-4 is highly expressed. In contrast, sfrp-2 is expressed in primitive tubules. To determine their role in kidney development, recombinant sFRP-1, sFRP-2 or combinations of both were applied to cultures of 13-dpc rat metanephroi. Both tubule formation and bud branching were markedly inhibited by sFRP-1, but concurrent sFRP-2 treatment restored some tubular differentiation and bud branching. sFRP-2 itself showed no effect on cultures of metanephroi. In cultures of isolated, induced rat metanephric mesenchymes, sFRP-1 blocked events associated with epithelial conversion (tubulogenesis and expression of lim-1, sfrp-2 and E-cadherin); however, it had no demonstrable effect on early events (compaction of mesenchyme and expression of wt1). As shown herein, sFRP-1 binds Wnt-4 with considerable avidity and inhibits the DNA-binding activity of TCF, an effector of Wnt signaling, while sFRP-2 had no effect on TCF activation. These observations suggest that sFRP-1 and sFRP-2 compete locally to regulate Wnt signaling during renal organogenesis. The antagonistic effect of sFRP-1 may be important either in preventing inappropriate development within differentiated areas of the medulla or in maintaining a population of cortical blastemal cells to facilitate further renal expansion. On the other hand, sFRP-2 might promote tubule formation by permitting Wnt-4 signaling in the presence of sFRP-1.
- Published
- 2001
20. Absence of Imprinting in U2AFBPL, a Human Homologue of the Imprinted Mouse GeneU2afbp-rs
- Author
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Christoph Plass, Kiyoji Okuda, Verne M. Chapman, Pieter DeJong, Kiyoshi Yoshino, William A. Held, Hideo Shibata, R. Scott Pearsall, Yoshihide Hayashizaki, and Anna Brozowska
- Subjects
Placenta ,Restriction Mapping ,Biophysics ,Nerve Tissue Proteins ,Biology ,Biochemistry ,Genomic Imprinting ,Mice ,Animals ,Humans ,Allele ,Imprinting (psychology) ,Molecular Biology ,Gene ,Alleles ,DNA Primers ,Repetitive Sequences, Nucleic Acid ,Synteny ,Genetics ,Binding protein ,Placental tissue ,Nuclear Proteins ,Cell Biology ,Ribonucleoproteins, Small Nuclear ,Splicing Factor U2AF ,Molecular biology ,Open reading frame ,Genes ,Ribonucleoproteins ,Chromosomes, Human, Pair 5 ,High homology - Abstract
The mouse gene U2 auxiliary factor binding protein related sequence ( U2afbp-rs ) has previously been shown to be genomically imprinted with monoallelic expression from the paternal allele. To determine if the human homologue is imprinted and contains conserved structural features which regulate imprinting, we isolated genomic clones from a human P1-derived artificial chromosome (PAC) library that map to human chromosome 5q22-31, a region syntenic to the proximal portion of mouse chromosome 11 where U2afbp-rs resides. A genomic subclone was isolated which contained an open reading frame with high homology to the mouse gene. This subclone also maintained the intronless character of the mouse gene. A Kpn I polymorphism within the open reading frame of the gene was found to occur in 21% (8/38) of the alleles tested from human placental tissue samples. RT-PCR analysis of human placentas using the Kpn I polymorphism to determine the parental origin of the alleles indicates biallelic expression of the human chromosome 5 U2AFBPL gene.
- Published
- 1996
21. IL-6, VTE and ovarian clear cell carcinoma: A dangerous triad
- Author
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Christina Fotopoulou, Takayuki Enomoto, Koji Matsuo, Yuji Ikeda, Kiyoshi Yoshino, Kosei Hasegawa, Ryusuke Murakami, L.D. Roman, Anil K. Sood, and Takeshi Hisamatsu
- Subjects
Oncology ,medicine.medical_specialty ,Triad (sociology) ,biology ,business.industry ,Internal medicine ,Clear cell carcinoma ,medicine ,biology.protein ,Obstetrics and Gynecology ,Interleukin 6 ,business - Published
- 2015
22. Lymphovascular space invasion and risk of lymph Node metastasis in epithelial ovarian cancer
- Author
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Karina E. Hew, Anil K. Sood, Neil B. Rosenshein, Dwight D. Im, Kiyoshi Yoshino, Takayuki Enomoto, L.D. Roman, Takahito Miyake, Koji Matsuo, and Todd B. Sheridan
- Subjects
Oncology ,medicine.medical_specialty ,business.industry ,Internal medicine ,Cancer research ,Obstetrics and Gynecology ,Medicine ,Epithelial ovarian cancer ,Lymph node metastasis ,business ,Lymphovascular - Published
- 2012
23. A successful case of abdominal radical trachelectomy for cervical cancer during pregnancy
- Author
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Takuji Tomimatsu, Yukari Miyoshi, Masami Fujita, Yutaka Ueda, Takayuki Enomoto, Kiyoshi Yoshino, Shinsuke Koyama, Tadashi Kimura, and Toshihiro Kimura
- Subjects
Gynecology ,Cervical cancer ,medicine.medical_specialty ,Pregnancy ,Reproductive Medicine ,Obstetrics ,business.industry ,medicine ,Obstetrics and Gynecology ,Trachelectomy ,business ,medicine.disease - Published
- 2011
24. The impact of multiple high-risk factors on survival outcome of surgically treated early-stage cervical cancer
- Author
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Kiyoshi Yoshino, Mika Okazawa, Seiji Mabuchi, L.D. Roman, S. Kamiura, Koji Matsuo, Tadashi Kimura, Yvonne G. Lin, and Yuri Matsumoto
- Subjects
Cervical cancer ,Oncology ,medicine.medical_specialty ,business.industry ,Internal medicine ,medicine ,Obstetrics and Gynecology ,High risk factors ,Stage (cooking) ,medicine.disease ,business ,Survival outcome - Published
- 2014
25. Impact of lymphovascular space invasion (LVSI) on survival of stage I epithelial ovarian cancer
- Author
-
Kiyoshi Yoshino, Chiaki Banzai, Todd B. Sheridan, Yasuhiko Shiki, Anil K. Sood, Masato Nishimura, L.D. Roman, Koji Matsuo, Kosei Hasegawa, and Kosuke Hiramatsu
- Subjects
Oncology ,business.industry ,Cancer research ,Obstetrics and Gynecology ,Medicine ,Epithelial ovarian cancer ,business ,Lymphovascular - Published
- 2014
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