1. A specific radioimmunoassay for Cortricotropin Releasing Factor (CRF) using synthetic ovine CRF
- Author
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Jingo Kageyama, Kozo Hashimoto, Zensuke Ota, Kazuharu Murakami, Norihito Ohno, Yoshiyuki Aoki, and Jiro Takahara
- Subjects
Male ,endocrine system ,medicine.medical_specialty ,Corticotropin-Releasing Hormone ,Hypothalamus ,Radioimmunoassay ,Neuropeptide ,Cross Reactions ,General Biochemistry, Genetics and Molecular Biology ,Corticotropin-releasing hormone ,Arcuate nucleus ,Internal medicine ,medicine ,Animals ,Tissue Distribution ,General Pharmacology, Toxicology and Pharmaceutics ,Brain Chemistry ,Sheep ,Suprachiasmatic nucleus ,Chemistry ,Microchemistry ,General Medicine ,Rats ,Endocrinology ,medicine.anatomical_structure ,Median eminence ,Chromatography, Gel ,Nucleus ,hormones, hormone substitutes, and hormone antagonists - Abstract
This newly developed specific radioimmunoassay for corticotropin releasing factor (CRF) had a sensitivity range of 25 pg/tube to 4 ng/tube. Intra and interassay coefficient of variation were 4.6% and 9.8%, respectively. Rat median eminence extracts showed a parallel dose response curve with synthetic ovine CRF and a significant cross reaction was not evident with other tested neuropeptides. The highest mean levels of CRF were found in the median eminence (6.61 ng/mg protein). Considerable amounts of CRF were found in the arcuate nucleus, paraventricular nucleus, dorsomedial nucleus, suprachiasmatic nucleus and ventromedial nucleus. The immunoreactive CRF of the rat medial basal hypothalamus coeluted with bioassayable CRF and with iodinated CRF on Sephadex G-75 chromatography. The results indicate that rat hypothalamus contains a CRF similar to ovine CRF.
- Published
- 1983
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