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Your search keyword '"Kajaste-Rudnitski A"' showing total 20 results
Start Over Author "Kajaste-Rudnitski A" Publisher elsevier bv
20 results on '"Kajaste-Rudnitski A"'

1. Removal of innate immune barriers allows efficient transduction of quiescent human hematopoietic stem cells

Author

Valeri, Erika, primary, Unali, Giulia, additional, Piras, Francesco, additional, Abou-Alezz, Monah, additional, Pais, Giulia, additional, Benedicenti, Fabrizio, additional, Lidonnici, Maria Rosa, additional, Cuccovillo, Ivan, additional, Castiglioni, Ilaria, additional, Arévalo, Sergio, additional, Spinozzi, Giulio, additional, Merelli, Ivan, additional, Behrendt, Rayk, additional, Oo, Adrian, additional, Kim, Baek, additional, Landau, Nathaniel R., additional, Ferrari, Giuliana, additional, Montini, Eugenio, additional, and Kajaste-Rudnitski, Anna, additional

Published
2024
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2. In vivo macrophage engineering reshapes the tumor microenvironment leading to eradication of liver metastases

Author

Kerzel, Thomas, primary, Giacca, Giovanna, additional, Beretta, Stefano, additional, Bresesti, Chiara, additional, Notaro, Marco, additional, Scotti, Giulia Maria, additional, Balestrieri, Chiara, additional, Canu, Tamara, additional, Redegalli, Miriam, additional, Pedica, Federica, additional, Genua, Marco, additional, Ostuni, Renato, additional, Kajaste-Rudnitski, Anna, additional, Oshima, Masanobu, additional, Tonon, Giovanni, additional, Merelli, Ivan, additional, Aldrighetti, Luca, additional, Dellabona, Paolo, additional, Coltella, Nadia, additional, Doglioni, Claudio, additional, Rancoita, Paola M.V., additional, Sanvito, Francesca, additional, Naldini, Luigi, additional, and Squadrito, Mario Leonardo, additional

Published
2023
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6. Choice of template delivery mitigates the genotoxic risk and adverse impact of editing in human hematopoietic stem cells

Author

Ferrari, Samuele, primary, Jacob, Aurelien, additional, Cesana, Daniela, additional, Laugel, Marianne, additional, Beretta, Stefano, additional, Varesi, Angelica, additional, Unali, Giulia, additional, Conti, Anastasia, additional, Canarutto, Daniele, additional, Albano, Luisa, additional, Calabria, Andrea, additional, Vavassori, Valentina, additional, Cipriani, Carlo, additional, Castiello, Maria Carmina, additional, Esposito, Simona, additional, Brombin, Chiara, additional, Cugnata, Federica, additional, Adjali, Oumeya, additional, Ayuso, Eduard, additional, Merelli, Ivan, additional, Villa, Anna, additional, Di Micco, Raffaella, additional, Kajaste-Rudnitski, Anna, additional, Montini, Eugenio, additional, Penaud-Budloo, Magalie, additional, and Naldini, Luigi, additional

Published
2022
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7. Laboratory-Scale Lentiviral Vector Production and Purification for Enhanced Ex Vivo and In Vivo Genetic Engineering

Author

Soldi, Monica, primary, Sergi Sergi, Lucia, additional, Unali, Giulia, additional, Kerzel, Thomas, additional, Cuccovillo, Ivan, additional, Capasso, Paola, additional, Annoni, Andrea, additional, Biffi, Mauro, additional, Rancoita, Paola Maria Vittoria, additional, Cantore, Alessio, additional, Lombardo, Angelo, additional, Naldini, Luigi, additional, Squadrito, Mario Leonardo, additional, and Kajaste-Rudnitski, Anna, additional

Published
2020
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8. Cyclosporin A and Rapamycin Relieve Distinct Lentiviral Restriction Blocks in Hematopoietic Stem and Progenitor Cells

Author

Eugenio Montini, Daniela Cesana, Carolina Petrillo, Luigi Naldini, Anna Kajaste-Rudnitski, Francesco Piras, Sara Bartolaccini, Petrillo, C., Cesana, D., Piras, F., Bartolaccini, S., Naldini, L., Montini, E., and Kajaste-Rudnitski, A.

Subjects
Genetic enhancement, Cellular differentiation, Transgene, Genetic Vectors, CD34, Gene Expression, Biology, Immunophenotyping, Mice, Transduction (genetics), Transduction, Genetic, Cyclosporin a, Drug Discovery, Genetics, Animals, Humans, Transgenes, Progenitor cell, Molecular Biology, Sirolimus, Pharmacology, Graft Survival, Lentivirus, Hematopoietic Stem Cell Transplantation, Cell Differentiation, Fetal Blood, Hematopoietic Stem Cells, Cell biology, Haematopoiesis, Phenotype, Immunology, Cyclosporine, Molecular Medicine, Original Article
Abstract

Improving hematopoietic stem and progenitor cell (HSPC) permissiveness to HIV-derived lentiviral vectors (LVs) remains a challenge for the field of gene therapy as high vector doses and prolonged ex vivo culture are still required to achieve clinically relevant transduction levels. We report here that Cyclosporin A (CsA) and Rapamycin (Rapa) significantly improve LV gene transfer in human and murine HSPC. Both compounds increased LV but not gammaretroviral transduction and acted independently of calcineurin and autophagy. Improved gene transfer was achieved across all CD34(+) subpopulations, including in long-term SCID repopulating cells. Effects of CsA were specific of HSPC and opposite to its known impact on HIV replication. Mutating the Cyclophilin A binding pocket of the viral capsid (CA) further improved transduction in combination with CsA. Tracking of the LV genome fate revealed that CsA relieves a CA-dependent early block and increases integration, while Rapa acts early in LV infection independently of the viral CA. In agreement, only Rapa was able to improve transduction by an integrase-defective LV harboring wild-type CA. Overall, our findings pave the way for more efficient and sustainable LV gene therapy in human HSPCs and shed light on the multiple innate barriers specifically hampering LV transduction in these cells.

Published
2015
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9. Cyclosporine H Overcomes Innate Immune Restrictions to Improve Lentiviral Transduction and Gene Editing In Human Hematopoietic Stem Cells

Author

Petrillo, Carolina, primary, Thorne, Lucy G., additional, Unali, Giulia, additional, Schiroli, Giulia, additional, Giordano, Anna M.S., additional, Piras, Francesco, additional, Cuccovillo, Ivan, additional, Petit, Sarah J., additional, Ahsan, Fatima, additional, Noursadeghi, Mahdad, additional, Clare, Simon, additional, Genovese, Pietro, additional, Gentner, Bernhard, additional, Naldini, Luigi, additional, Towers, Greg J., additional, and Kajaste-Rudnitski, Anna, additional

Published
2018
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10. Efficient Ex Vivo Engineering and Expansion of Highly Purified Human Hematopoietic Stem and Progenitor Cell Populations for Gene Therapy

Author

Zonari, Erika, primary, Desantis, Giacomo, additional, Petrillo, Carolina, additional, Boccalatte, Francesco E., additional, Lidonnici, Maria Rosa, additional, Kajaste-Rudnitski, Anna, additional, Aiuti, Alessandro, additional, Ferrari, Giuliana, additional, Naldini, Luigi, additional, and Gentner, Bernhard, additional

Published
2017
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11. 36. Genome-Wide Insight Into the Transcriptional Modulations Triggered By Lentiviral Transduction in Human Hematopoietic Stem Cells

Author

Dejan Lazarevic, Elia Stupka, Michela Riba, Anna Kajaste-Rudnitski, Luigi Naldini, Davide Cittaro, Francesco Piras, and Sara Bartolaccini

Subjects
Pharmacology, Genetic enhancement, CD34, Biology, Molecular biology, Cell biology, Transduction (genetics), Haematopoiesis, Multiplicity of infection, Drug Discovery, Genetics, Molecular Medicine, Stem cell, KEGG, Progenitor cell, Molecular Biology
Abstract

Recent studies suggest that hematopoietic stem cells (HSC) can sense foreign nucleic acids and pathogen-associated molecular patterns (PAMPs). Exposure to lentiviral vectors (LV) upon gene transfer may thus trigger acute host responses in HSC that could potentially impact on their biological properties, although no comprehensive studies are available to date. We have performed a high throughput RNA-Seq analysis on human cord-blood (CB)-derived CD34+ hematopoietic stem and progenitor cells (HSPC) exposed to research- or clinical-grade VSV-g pseudotyped (SIN) LV at a high multiplicity of infection, matching current clinical vector dose requirements. As controls, cells were exposed to non-transducing Env-less, genome-less or heat inactivated control vectors or kept in culture untreated. RNA was extracted at different times early after transduction, processed and ran in Illumina HiSeq2000. Analysis of Differential Expression in Time Course was performed using LIMMA R/BioConductor library. Key pathways were assessed by Term Enrichment Analysis considering KEGG pathways and Gene Ontology Biological processes. Transduction with both research-and clinical-grade LV significantly triggered DNA damage and apoptosis-related responses. In particular, p53 signaling was among the most significantly altered pathways (p

Published
2015
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12. The 2′,5′-Oligoadenylate Synthetase 1b Is a Potent Inhibitor of West Nile Virus Replication Inside Infected Cells

Author

Marianne Lucas, Jean Louis Guénet, Marie Pascale Frenkiel, Anna Kajaste-Rudnitski, Tomoji Mashimo, and Philippe Desprès

Subjects
Proteasome Endopeptidase Complex, Cell Survival, RNase P, viruses, Blotting, Western, Molecular Sequence Data, Endoribonuclease, Tetrazolium Salts, Transfection, Virus Replication, Antiviral Agents, Sensitivity and Specificity, Biochemistry, Cell Line, Mice, Viral life cycle, Endoribonucleases, 2',5'-Oligoadenylate Synthetase, Animals, Immunoprecipitation, Protease Inhibitors, Amino Acid Sequence, Fluorescent Antibody Technique, Indirect, Molecular Biology, Gene, DNA Primers, Base Sequence, biology, Reverse Transcriptase Polymerase Chain Reaction, 2'-5'-Oligoadenylate, Immunity, RNA, RNA virus, 3T3 Cells, Cell Biology, Fibroblasts, biology.organism_classification, Virology, Blotting, Southern, Thiazoles, Culicidae, Viral replication, RNA, Viral, Interferons, West Nile virus, West Nile Fever
Abstract

The 2',5'-oligoadenylate synthetase (OAS) proteins associated with endoribonuclease RNase L are components of the interferon-regulated OAS/RNase L system, which is an RNA decay pathway known to play an important role in the innate antiviral immunity. A large body of evidence suggests a critical role for the 1b isoform of the mouse Oas gene (Oas1b) in resistance to West Nile virus (WNV) infection in vivo. WNV is a positive, single-stranded RNA virus responsible for severe encephalitis in a large range of animal species and humans. To investigate the molecular basis for the sensitivity of WNV to the Oas1b antiviral pathway, we established a stable mouse fibroblastic cell clone that up-regulates Oas1b protein expression under the control of the Tet-Off expression system. We showed that murine cells respond to Oas1b expression by efficiently inhibiting WNV replication. The antiviral action of Oas1b was essentially restricted to the early stages in virus life cycle. We found that the inability of WNV to productively infect the Oas1b-expressing cells was attributable to a dramatic reduction in positive-stranded viral RNA level. Thus, Oas1b represents an antiviral pathway that exerts its inhibitory effect on WNV replication by preventing viral RNA accumulation inside infected cells.

Published
2006

13. 288. Dual-Regulated Lentiviral Vector for Gene Therapy of X-Linked Chronic Granulomatous Disease

Author

Giada Farinelli, Maria Chiriaco, Valentina Capo, Erika Zonari, Maddalena Migliavacca, Raisa Jofra Hernandez, Samantha Scaramuzza, Gigliola Di Matteo, Lucia Sergi Sergi, Alice Rossi, Serena Ranucci, Alessandra Bragonzi, Anna Kajaste-Rudnitski, Didier Trono, Manuel Grez, Paolo Rossi, Andrea Finocchi, Luigi Naldini, Bernhard Gentner, and Alessandro Aiuti

Subjects
Pharmacology, Drug Discovery, Genetics, Molecular Medicine, Molecular Biology
Published
2015
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16. 288. Dual-Regulated Lentiviral Vector for Gene Therapy of X-Linked Chronic Granulomatous Disease

Author

Farinelli, Giada, primary, Chiriaco, Maria, additional, Capo, Valentina, additional, Zonari, Erika, additional, Migliavacca, Maddalena, additional, Jofra Hernandez, Raisa, additional, Scaramuzza, Samantha, additional, Di Matteo, Gigliola, additional, Sergi Sergi, Lucia, additional, Rossi, Alice, additional, Ranucci, Serena, additional, Bragonzi, Alessandra, additional, Kajaste-Rudnitski, Anna, additional, Trono, Didier, additional, Grez, Manuel, additional, Rossi, Paolo, additional, Finocchi, Andrea, additional, Naldini, Luigi, additional, Gentner, Bernhard, additional, and Aiuti, Alessandro, additional

Published
2015
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18. Dual-regulated Lentiviral Vector for Gene Therapy of X-linked Chronic Granulomatosis

Author

Chiriaco, Maria, primary, Farinelli, Giada, additional, Capo, Valentina, additional, Zonari, Erika, additional, Scaramuzza, Samantha, additional, Di Matteo, Gigliola, additional, Sergi, Lucia Sergi, additional, Migliavacca, Maddalena, additional, Hernandez, Raisa Jofra, additional, Bombelli, Ferdinando, additional, Giorda, Ezio, additional, Kajaste-Rudnitski, Anna, additional, Trono, Didier, additional, Grez, Manuel, additional, Rossi, Paolo, additional, Finocchi, Andrea, additional, Naldini, Luigi, additional, Gentner, Bernhard, additional, and Aiuti, Alessandro, additional

Published
2014
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