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Made available in DSpace on 2019-10-06T17:07:01Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-06-01 Aim: This study evaluated the effects of topical green tea extract solution (GTE) as adjuvant therapy to mechanical debridement for the treatment of experimental periodontitis (EP). Material and methods: We used 120 male rats (Rattus norvegicus albinus – Wistar), divided into the following four groups: NEP (sham) (n = 30): no experimental periodontitis (NEP), only simulation of EP by installation and removal of a ligature; EP (n = 30): EP induction by ligature; SRP (n = 30): EP, scaling and root planing (SRP), and irrigation with physiological saline solution; SRP/GT (n = 30): EP, SRP, and irrigation with GTE. Histologic analysis and immunohistochemistry were performed for detection of interleukin (IL)1ß, tumor necrosis factor-alpha (TNF-α), IL-10, and anti-tartrate resistant acid phosphatase (TRAP) in the furcation area. The percentage of bone in the furcation (PBF) was considered the primary variable and evaluated at 14, 22, and 37 days. The data from the histological analysis and the IL- 1B, TNF- A, and IL-10 were submitted to a Kruskal-Wallis variance test and Dunn's posttest (p ≤ 0.05). The histometric data and TRAP were submitted to analysis of variance (ANOVA) and Tukey's posttest (p ≤ 0.05). Results: The SRP/GT group showed lower inflammatory process, lower immunolabeling pattern of IL-1ß and TNF-α, and greater immunolabeling pattern of IL-10 compared with the EP and SRP groups at 22 days. Fewer TRAP-positive multinucleated osteoclasts were observed in all periods in the SRP/GT group (5.22 ± 0.65; 7.33 ± 0.80; 8.55 ± 1.15) compared with the SRP group (30.67 ± 8.55; 13.22 ± 0.77; 13.87 ± 0.77). Higher PBF was observed in all periods in the SRP/GT group (74.65 ± 7.14; 76.61 ± 5.36; 79.24 ± 3.75) compared with the SRP group (61.60 ± 9.48; 54.84 ± 9.06; 53.25 ± 9.66). Conclusion: GTE adjuvant to SRP reduced inflammation, osteoclastic activity, and alveolar bone loss in EP. São Paulo State University (UNESP) School of Dentistry Department of Surgery and Integrated Clinic – Periodontics Division São Paulo State University (UNESP) School of Dentistry Department of Basic Science – Histology Division São Paulo State University (UNESP) School of Dentistry Department of Surgery and Integrated Clinic – Periodontics Division São Paulo State University (UNESP) School of Dentistry Department of Basic Science – Histology Division

Made available in DSpace on 2019-10-06T16:53:04Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-03-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) This study evaluated the effects of antimicrobial photodynamic therapy (aPDT) in the alveolar repair of rats with major risk factors for bisphosphonate-related osteonecrosis of the jaws (BRONJ). Senile rats received 0.45 ml of vehicle (VEH and VEH/aPDT) or 0.45 ml of zoledronate (ZOL and ZOL/aPDT) every three days for seven weeks. After three weeks of treatment, the first lower left molar was extracted. VEH/aPDT and ZOL/aPDT were submitted to aPDT on the extraction site at 0, 2 and 4 days postoperatively. Euthanasia was performed 28 days postoperatively and the extraction site was evaluated by clinical, histological, histometric, histochemical and immunohistochemical analysis. ZOL showed tissue repair impairment; lower percentage of newly formed bone tissue (NFBT); higher percentage of non-vital bone tissue (NVBT); fewer mature collagen fibers and increased immunolabeling for tumor necrosis factor (TNFα), interleukin (IL)-1β and IL-6. ZOL/aPDT showed clinical and histological characteristics of the extraction site, percentage of NFBT and percentage of mature collagen fiber similar to VEH. Percentage of NVBT and immunolabeling for inflammatory cytokines in ZOL/aPDT was lower than in ZOL. Immunolabeling for tartarato-resistant acid phosphatase (TRAP) was lower in ZOL and ZOL/aPDT. aPDT in the dental extraction site improves tissue repair process and prevents the occurrence of BRONJ-like lesions after tooth extraction. São Paulo State University (UNESP) School of Dentistry Department of Basic Sciences, Rua José Bonifácio, 1193 São Paulo State University (UNESP) Institute of Biosciences, Rua Prof. Dr. Antônio Celso Wagner Zanin, 250 São Paulo State University (UNESP) School of Dentistry Department of Surgery and Integrated Clinic, Rua José Bonifácio, 1193 São Paulo State University (UNESP) School of Dentistry Department of Pediatric Dentistry and Public Health, Rua José Bonifácio, 1193 São Paulo University (USP) School of Dentistry Department of Morphology Physiology and Basic Pathology, Avenida do Café s/n Group for the Research and Study of Laser in Dentistry São Paulo State University (UNESP) School of Dentistry, Rua José Bonifácio, 1193 São Paulo State University (UNESP) School of Dentistry Department of Basic Sciences, Rua José Bonifácio, 1193 São Paulo State University (UNESP) Institute of Biosciences, Rua Prof. Dr. Antônio Celso Wagner Zanin, 250 São Paulo State University (UNESP) School of Dentistry Department of Surgery and Integrated Clinic, Rua José Bonifácio, 1193 São Paulo State University (UNESP) School of Dentistry Department of Pediatric Dentistry and Public Health, Rua José Bonifácio, 1193 Group for the Research and Study of Laser in Dentistry São Paulo State University (UNESP) School of Dentistry, Rua José Bonifácio, 1193 FAPESP: 2012/03067-6 FAPESP: 2013/25367-4 FAPESP: 2013/26779-4 FAPESP: 2014/02199-1 FAPESP: 2015/22395-2 CNPq: 28040-2013 CNPq: 30699-2014

Made available in DSpace on 2018-12-11T17:37:02Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-07-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) The aim of this study was to evaluate the effects of photobiomodulation (PBM) with multiple sessions of low-level laser on the alveolar repair process of rats with major risk factors for medication-related osteonecrosis of the jaws (MRONJ). Senile rats received 0.45 mL of vehicle (VEH and VEH-PBM) or 0.45 mL of 100 μg/kg zoledronate (ZOL and ZOL-PBM) administrated intraperitoneally every two days during seven weeks. After three weeks of initiation of drug treatment the first lower left molar was extracted. No local treatment was performed in VEH and ZOL. VEH-PBM and ZOL-PBM were submitted to laser irradiation (660 ± 10 nm; 0.035 W; 2.1 J; 60 s) on the extraction site at 0, 2 and 4 days postoperatively. Euthanasia was performed 28 days after tooth extraction. Histological sections of the hemimandible were submitted to histopathological and histomorphometric analysis, as well as to histochemistry for collagen fiber maturation and immunohistochemistry for pro-inflammatory cytokines. In ZOL, general impairment of tissue repair, areas with osteonecrosis, lower newly formed bone tissue (NFBT), smaller amount of mature collagen fibers and increased immunoreactivity for TNFα IL-1β and IL-6 were observed when compared to VEH and VEH-PBM. ZOL-PBM showed significant improvement in some parameters compared to ZOL, such as positive repair tissue, higher NFBT, greater amount of mature collagen fibers, besides TNFα and IL-1β immunoreactivity decrease. Zoledronate treatment severely compromised the tissue repair process of the tooth extraction site in rats with major risk factors for MRONJ. Based on parameters employed in the present study, PBM in multiple sessions can improve the alveolar repair process, constituting a promising preventive therapy to avoid the onset of post-extraction MRONJ. Group for the Research and Study of Laser in Dentistry School of Dentistry of Araçatuba São Paulo State University (UNESP) Department of Surgery and Integrated Clinic School of Dentistry São Paulo State University (UNESP), Rua José Bonifácio, 1193, CEP Department of Basic Science School of Dentistry São Paulo State University (UNESP), Rua José Bonifácio, 1193, CEP Institute of Biosciences São Paulo State University (UNESP), Rua Prof. Dr. Antônio Celso Wagner Zanin, 250, CEP Department of Morphology Physiology and Basic Pathology School of Dentistry São Paulo University (USP), Avenida do Café s/n, CEP Department of Restorative Dentistry School of Dentistry São Paulo StateUniversity (UNESP), Rua José Bonifácio, 1193, CEP Group for the Research and Study of Laser in Dentistry School of Dentistry of Araçatuba São Paulo State University (UNESP) Department of Surgery and Integrated Clinic School of Dentistry São Paulo State University (UNESP), Rua José Bonifácio, 1193, CEP Department of Basic Science School of Dentistry São Paulo State University (UNESP), Rua José Bonifácio, 1193, CEP Institute of Biosciences São Paulo State University (UNESP), Rua Prof. Dr. Antônio Celso Wagner Zanin, 250, CEP Department of Restorative Dentistry School of Dentistry São Paulo StateUniversity (UNESP), Rua José Bonifácio, 1193, CEP FAPESP: #2012/03067-6 FAPESP: #2013/26779-4 FAPESP: #2014/02199-1 FAPESP: #2014/14118-6 CNPq: #28040-2013 CNPq: #30699-2014

Despite clinical trials and reviews attempt to assess a possible relationship between dementia and periodontal disease, no meta-analysis has been performed and this issue remains undetermined. The aim of this study is to conduct a systematic review and meta-analysis to assess severity of periodontitis in subjects with dementia.The search was conducted in Pubmed, Embase/MEDLINE. Two independent reviewers extracted data and assessed the risk bias (Newcastle-Ottawa scale). Meta-analyses were performed using the means of probing depth (PD) and clinical attachment loss (CAL) in patients with or without dementia. The mean difference were analyzed (P ≤ 0.05).Fourteen studies were included in the systematic review. In the qualitative analysis, most studies reported higher prevalence of periodontal disease in dementia patients. The studies had low risk of bias and two meta-analyses were performed for each parameter, including or not a cross-sectional study. The meta-analyses including the cross-sectional study demonstrated significant association between dementia and periodontal disease (mean difference: PD = 1.41; CAL = 1.40, P 0.05), however, it wasn't confirmed when the cross-sectional study was removed (1.25 mm, P 0.22) and CAL (1.20 mm, P 0.22).Although the qualitative analysis have suggested worse periodontal conditions in dementia patients, due to different study types and the high heterogeneity among them, the meta-analysis does not support the association between dementia and severity of periodontal disease.

Made available in DSpace on 2018-12-11T17:05:36Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-10-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Objective To histomorphometrically analyze the effect of low-level laser therapy (LLLT) on bone formation process in surgically created critical-size defects (CSDs) treated with bovine bone graft (BBG) and its influence over particles' resorption of BBG. Methods A 10-mm diameter CSD was surgically created in the calvaria of 64 male rats, which were distributed into 4 experimental groups: the C group (control), only blood clot; the LLLT group, LLLT (GaAlAs, 660 nm) and blood clot; the BBG group, CSD filled with BBG; the BBG/LLLT group, LLLT and CSD filled with BBG. Animals were euthanized at either 30 or 60 days post-operation. A histological analysis was performed. Additionally, the percentage of newly formed bone area (NFBA) and remaining particles areas (RPA) of BBG were histometrically evaluated and data statistically analyzed. Results The LLLT (5.82 ± 2.05; 7.34 ± 1.01) group presented significantly greater NFBA when compared to the C group (1.61 ± 0.30; 5.59 ± 0.94) at 30 and 60 days post-operation (p

The aim of this study was to evaluate the effects of low-level laser therapy (LLLT) on the bone repair of critical size defects (CSDs) filled with autogenous bone in the calvaria of immunosuppressed rats. A 5 mm-diameter CSD was created in the calvaria of 30 rats. The animals were divided into 5 groups (n = 6): Control (C)--the defect was filled with a blood clot; Dexamethasone (D)--dexamethasone treatment, and the defect was filled with a blood clot; Autogenous bone (AB)--dexamethasone treatment, and the defect was filled with autogenous bone; LLLT--dexamethasone treatment, and the defect received LLLT (660 nm; 35 mW; 24.7 J/cm(2)); and AB + LLLT--dexamethasone treatment, and the defect was filled with autogenous bone and received LLLT. All animals were euthanized at 30 postoperative days. Histometric and histological analyses were performed. The new bone area (NBA) was calculated as the percentage of the total area of the original defect. Data were analysed statistically (an analysis of variance and Tukey's test; P < 0.05). The AB + LLLT group showed the largest NBA of all groups (P < 0.05). The use of LLLT with AB effectively stimulated bone formation in CSDs in the calvaria of immunosuppressed rats.