Search

Your search keyword '"Juan Miguel Villalobos Salcedo"' showing total 7 results
Start Over Author "Juan Miguel Villalobos Salcedo" Publisher elsevier bv
7 results on '"Juan Miguel Villalobos Salcedo"'

1. Hepatitis D double reflex testing of all hepatitis B carriers in low-HBV- and high-HBV/HDV-prevalence countries

Author

Homie A. Razavi, Maria Buti, Norah A. Terrault, Stefan Zeuzem, Cihan Yurdaydin, Junko Tanaka, Alessio Aghemo, Ulus S. Akarca, Nasser M. Al Masri, Abduljaleel M. Alalwan, Soo Aleman, Abdullah S. Alghamdi, Saad Alghamdi, Waleed K. Al-Hamoudi, Abdulrahman A. Aljumah, Ibrahim H. Altraif, Tarik Asselah, Ziv Ben-Ari, Thomas Berg, Mia J. Biondi, Sarah Blach, Wornei S.M. Braga, Carlos E. Brandão-Mello, Maurizia R. Brunetto, Joaquin Cabezas, Hugo Cheinquer, Pei-Jer Chen, Myeong-Eun Cheon, Wan-Long Chuang, Carla S. Coffin, Nicola Coppola, Antonio Craxi, Javier Crespo, Victor De Ledinghen, Ann-Sofi Duberg, Ohad Etzion, Maria Lucia G. Ferraz, Paulo R.A. Ferreira, Xavier Forns, Graham R. Foster, Giovanni B. Gaeta, Ivane Gamkrelidze, Javier García-Samaniego, Liliana S. Gheorghe, Pierre M. Gholam, Robert G. Gish, Jeffrey Glenn, Julian Hercun, Yao-Chun Hsu, Ching-Chih Hu, Jee-Fu Huang, Naveed Janjua, Jidong Jia, Martin Kåberg, Kelly D.E. Kaita, Habiba Kamal, Jia-Horng Kao, Loreta A. Kondili, Martin Lagging, Pablo Lázaro, Jeffrey V. Lazarus, Mei-Hsuan Lee, Young-Suk Lim, Paul J. Marotta, Maria-Cristina Navas, Marcelo C.M. Naveira, Mauricio Orrego, Carla Osiowy, Calvin Q. Pan, Mário G. Pessoa, Giovanni Raimondo, Alnoor Ramji, Devin M. Razavi-Shearer, Kathryn Razavi-Shearer, Cielo Y. Ríos-Hincapié, Manuel Rodríguez, William M.C. Rosenberg, Dominique M. Roulot, Stephen D. Ryder, Rifaat Safadi, Faisal M. Sanai, Teresa A. Santantonio, Christoph Sarrazin, Daniel Shouval, Frank Tacke, Tammo L. Tergast, Juan Miguel Villalobos-Salcedo, Alexis S. Voeller, Hwai-I Yang, Ming-Lung Yu, and Eli Zuckerman

Subjects
Hepatology
Published
2023

2. P-48 HEPATITIS DELTA: THE MOST SEVERE OF ALL VIRAL HEPATITIS

Author

Júlia Teixeira Ton, Deusilene Vieira Dallácqua, Alcione de Oliveira dos Santos, Juan Miguel Villalobos Salcedo, Mariana Pinheiro Alves Vasconcelos, and Ester Teixeira Ton

Subjects
Hepatitis, medicine.medical_specialty, Hepatology, business.industry, viruses, Public health, Specialties of internal medicine, virus diseases, Retrospective cohort study, General Medicine, biochemical phenomena, metabolism, and nutrition, medicine.disease, Chronic liver disease, digestive system diseases, Liver disease, RC581-951, Internal medicine, Tropical medicine, medicine, Portal hypertension, business, Viral hepatitis
Abstract

Introduction: 500,000 to 1.2 million deaths are speculated annually from complications of hepatitis B. The hepatitis Delta virus (HDV) also represents an important public health problem in endemic areas. Objective: To analyze the clinical and laboratory characteristics at the first consultation of HBV and HBV/HDV patients. Methods: Retrospective study (2017 and 2018) of 324 records of HBV and HBV/HDV patients at Research Center for Tropical Medicine of Rondônia. Project approved by the Research Ethics Committee. For statistical analysis, SPSS® version 25.0. Results: A total of 324 patients were included, 302 (93.2%) were HBV and 22 were (6.7%) HBV/HDV. At the first consultation, 16.2% of the HBV showed signs of chronic liver disease, while in the HBV/HDV patients, 59.1% (p

Published
2021

5. Development of a reverse transcription quantitative real-time PCR-based system for rapid detection and quantitation of hepatitis delta virus in the western Amazon region of Brazil

Author

Eduardo Resende Honda, Juan Miguel Villalobos-Salcedo, Alcione de Oliveira dos Santos, Luan Felipo Botelho-Souza, Lourdes Maria Borzacov, and Deusilene Souza Vieira

Subjects
HBsAg, viruses, Hepatitis C virus, Biology, medicine.disease_cause, Real-Time Polymerase Chain Reaction, Virus, Virology, medicine, Humans, Hepatitis B virus, Reverse Transcriptase Polymerase Chain Reaction, virus diseases, biochemical phenomena, metabolism, and nutrition, Viral Load, Quantitative real-time PCR, Reverse transcriptase, Hepatitis D, HDV RNA, Real-time polymerase chain reaction, Viral replication, Molecular Diagnostic Techniques, Hepatitis Delta Virus, Viral load, Brazil, Hydrolysis probe
Abstract

The hepatitis delta virus (HDV) is a pathogen that causes a severe and rapidly progressive disease of hepatocytes. The measurement of viral load in the peripheral blood of patients with HDV infections is important for diagnosis, treatment monitoring, and support for follow-up studies of viral replication during the course of the disease. This study reports the development of an assay capable of detecting and quantifying the abundance of HDV particles in serum samples, based on reverse-transcription quantitative PCR (RT-qPCR). Two standards for calibration were produced for determining the viral load of HDV: a cDNA cloned into a linear plasmid and a transcribed RNA. For validating this assay, 140 clinical samples of sera were used, comprising 100 samples from patients who tested positive for anti-HDV and hepatitis B virus surface antigen (HBsAg) by ELISA; 30 samples from blood donors; 5 samples monoinfected with hepatitis B virus (HBV); and 5 samples monoinfected with hepatitis C virus (HCV). The HDV RT-qPCR assay performed better when calibrated using the standard based on HDV cDNA cloned into a linear plasmid, yielding an efficiency of 99.8% and a specificity of 100% in the in vitro assays. This study represents the first HDV RT-qPCR assay developed with clinical samples from Brazil and offers great potential for new clinical efficacy studies of antiviral therapeutics for use in patients with hepatitis delta in the western Amazon region.

Published
2014
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results