1. Mentha × piperita L. nodal segments cultures and their essential oil production
- Author
-
Vincenzo De Feo, Daniela Gruľová, Bohuš Obert, Anna Preťová, Eva Ürgeová, and Jozef Fejér
- Subjects
0106 biological sciences ,Menthofuran ,Biology ,01 natural sciences ,law.invention ,Essential oil ,Growth regulator ,chemistry.chemical_compound ,Growth elicitor ,law ,010608 biotechnology ,Peppermint ,Peppermint, *Growth regulator, *Growth elicitor, *Pulegone, *Menthofuran, *Essential oil ,food and beverages ,Pulegone ,Horticulture ,chemistry ,Shoot ,Kinetin ,Composition (visual arts) ,NODAL ,Agronomy and Crop Science ,010606 plant biology & botany - Abstract
The aim of the present study was the achievement of sterile shoot cultures from peppermint (Mentha × piperita L. cv. ʻKristinkaʼ), their multiplication and the evaluation of the production of essential oils in in vitro conditions. The study was carried out by culturing the nodal segments both on basal or on media supplemented with different concentrations of growth regulators (2,4-D, kinetin, BAP, ZEA) or elicitors (CoCl2). The growth of nodal segments did not showed differences after subculturing, neither when cultivated on media supplemented with 2,4-D or CoCl2. The supplementation of 0.5 mg L−1 BAP caused an increase in the number of de novo formed shoots. The composition of the essential oils showed remarkable differences, with an enhancement of the percentage of menthofuran after the supplementation of 2,4-D and CoCl2 or of pulegone after application of BAP and ZEA.
- Published
- 2018
- Full Text
- View/download PDF