1. Evaluation of MALDI-ToF as a method for the identification of bacteria in the veterinary diagnostic laboratory
- Author
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Mark S. Koylass, Fabrizio Lemma, Peter Wragg, Danny Worth, Luke P. Randall, Roger D. Ayling, Andrew Steventon, Kirsty Line, Markus Kostrzewa, Monika Klita, Adrian M. Whatmore, Jon Rogers, and Jakub Muchowski
- Subjects
Veterinary Medicine ,Veterinary medicine ,Bacteria ,Formates ,General Veterinary ,Reproducibility of Results ,Bacterial Infections ,Gold standard (test) ,Biology ,biology.organism_classification ,Mass spectrometry ,16S ribosomal RNA ,Microbiology ,Species Specificity ,RNA, Ribosomal, 16S ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,parasitic diseases ,Animals ,Diagnostic laboratory ,RNA RIBOSOMAL 16S - Abstract
Matrix-Assisted Laser Desorption Ionisation-Time of Flight (MALDI-ToF) Mass Spectrometry with Bruker MALDI Biotyper software was evaluated as a method for identifying veterinary bacteria. For 620 isolates (~100 bacterial species), identification by MALDI-ToF and non-16S rDNA methods (mainly phenotypic/biochemical) agreed to species-level (95.3%) and to species/genus-level (100%), but in the absence of 16S rDNA as a gold standard. For a further panel of 107 anaerobes and 234 aerobes (~100 bacteria species) using 16S rDNA results as the gold standard, MALDI-ToF/biochemical tests showed 97.8/96.6% species-level and 99.6/93.5% genus-level agreement for aerobes and 95.3/93.6% species-level and 100/95.3% genus-level agreement for anaerobes compared to the gold standard. Where results were obtained from direct spots, direct spots overlaid with formic acid and extracts, 89.4% of 180 aerobes and 90.1% of 152 anaerobes were identified by MALDI-ToF. MALDI-ToF was shown to be a rapid and reliable method to identify veterinary bacteria.
- Published
- 2015