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Tree-ring stable carbon isotope ratios (δ13C) often display a decline over the industrial period (post-AD1850) that is only partly explained by changes in the isotopic ratio of carbon dioxide (CO2) and may represent a response to increased atmospheric concentrations of CO2 (ca). If this is not addressed, reconstructions using long tree-ring stable isotope chronologies calibrated using the modern period, for which meteorological records are available, may be compromised. We propose a correction procedure that attempts to calculate the δ13C values that would have been obtained under pre-industrial conditions. The correction procedure uses nonlinear (loess) regression but the magnitude of the adjustment made is restricted by two logical constraints based on the physiological response of trees: first, that a unit increase in ca cannot result in more than the same unit increase in the internal concentration of CO2 (ci), and second, that increases in water-use efficiency as a result of an increase in ca are limited to maintaining a constant ci/ca ratio. The first constraint allows retention of a falling trend in δ13C, which exceeds that which could logically be attributed to a passive response to rising ca. The second constraint ensures that any increase in δ13C, reflecting a change in water-use efficiency beyond maintenance of a constant ci/ca, is not removed. The procedure is tested using ‘pseudoproxies’, to demonstrate the effect of the correction on time-series with different shapes, and data from three sites in Finland and Norway. Two of the time-series retain a significant trend after correction, and in all three cases the correction improves the correlation with local meteorological measurements.

α-Cellulose was isolated from four samples of Scots pine (Pinus sylvestris L.). Each sample was divided into two portions. One portion had the resins removed by solvent extraction prior to removal of lignins by treatment with acidic sodium chlorite solution and treatment with sodium hydroxide solution to remove hemicelluloses. The other portion was processed in the same way apart from the solvent extraction step. The isolated wood constituents were characterised by attenuated total reflectance Fourier transform infrared (ATR/FT-IR) spectroscopy. The infrared spectra of the resulting α-cellulose samples were identical indicating that treatment with acidic sodium chlorite and sodium hydroxide was sufficient to remove resins. The values of the stable isotope ratios (carbon, oxygen and hydrogen) for each pair of α-cellulose sub-samples also showed no significant differences within the reproducibility of the methods. The implication of these studies demonstrate that the customary step of resin extraction from pine is unnecessary if sodium chlorite and sodium hydroxide are used for the isolation of α-cellulose following the technique described in this paper. In addition, the study demonstrates that the oxygen isotope ratio of the water used for cellulose extraction does not influence the stable isotope values in the α-cellulose obtained. The importance of isotopic homogeneity within the cellulose sample is also highlighted.

Annual latewood δ18O values from oak (Quercus robur L.) are presented for the period 1895–1994 for four trees growing in eastern England. We demonstrate, using the δ18O values of precipitation from Wallingford (1982–1994) and the longer time-series from Groningen (1965–1994), that the δ18O values of winter precipitation had the strongest influence on the δ18O values of tree-ring cellulose. The influence of winter precipitation on the spring/summer formed latewood may be explained by several factors: most soil water is replenished during the winter, water may take several months to penetrate the unsaturated zone, and oaks may sample water from lower in the profile during times of water stress. The δ18O values of oak latewood cellulose were also significantly (P