Search

Calcium phosphate bio-ceramics are osteo-conductive, but it remains a challenge to promote the induction of bone augmentation and capillary formation. The surface micro/nano-topography of materials can be recognized by cells and then the cell fate are mediated. Traditional regulation methods of carving surface structures on bio-ceramics employ mineral reagents and organic additives, which might introduce impurity phases and affect the biological results. In a previous study, a facile and novel method was utilized with ultrapure water as the unique reagent for hydrothermal treatment, and a uniform hydroxyapatite (HAp) surface layer was constructed on composite ceramics (β-TCP/CaSiO3) in situ. Further combined with 3D printing technology, biomimetic hierarchical structure scaffolds were fabricated with interconnected porous composite ceramic scaffolds as the architecture and micro/nano-rod hybrid HAp as the surface layer. The obtained HAp surface layer favoured cell adhesion, alleviated the cytotoxicity of precursor scaffolds, and upregulated the cellular differentiation of mBMSCs and gene expression of HUVECs in vitro. In vivo studies showed that capillary formation, bone augmentation and new bone matrix formation were upregulated after the HAp surface layer was obtained, and the results confirmed that the fabricated biomimetic hierarchical structure scaffold could be an effective candidate for bone regeneration., Graphical abstract The brief illustration of the study. The architecture of the scaffolds was fabricated from the 3D printing, micro/nano-rod hybrid HAp surface were obtained via hydrothermal treatment with a dissolution-precipitation stage. Biomimetic hierarchical structure scaffolds were fabricated with interconnected porous composite ceramics scaffolds as the architecture and micro/nano-rod hybrid HAp as the surface. Angiogenesis and osteogenesis of the biomimetic hierarchical structure scaffolds were studied in vitro (hUVECs and mBMSCs) and in vivo (operated in SD rat subcutaneous implant in backs and the extramembranous implant near the calvaria.).Image 1, Highlight • Simple and practical process to construct surface structure layer in situ with little impurities. • Combined with the 3D printing technology to fabricate architecture of the pre-treated matrix. • Study the angiogenesis and osteogenesis (for mesenchymal stem cells) separately. • Improving tissue growth in vivo: capillary formation, bone-augmentation and new bone matrix formation.

The energy consumed by urban transportation systems has implications for local environmental protection and greenhouse gas emission reductions. It is widely claimed that in growing cities, individuals' transport energy use could be made more efficient by planning to control urban sprawl and create polycentric urban structures. However, existing conclusions are mixed. This paper contributes to this issue with an in-depth analysis of China's cities. Interestingly, polycentric cities demonstrated lower travel energy efficiency than monocentric ones. This is mainly because urban sub-center developments have failed to combine employment and residential land use. In these planned sub-centers, land use is usually dominated by either housing or industrial park developments, requiring people to commute long distances between home and work, and use cars at high rates. Increasing fragmentation of development management due to political decentralization has apparently worsened the job-housing imbalance. Though a significant effect of urban structure on transport energy consumption was observed, car use control policies had no effect, while a high level of metro services was associated with lower energy consumption.

Hollow mesoporous bioactive glass microspheres (HMBGMs) were fabricated via a hydrothermal-assisted self-transformation method employing cetyltrimethyl ammonium bromide (CTAB) as a mesoporous template. The morphology, structure, particle size and specific surface area of HMBGMs were characterized by various methods. The results demonstrated that the solid BG spheres prepared in a Sto¨ber solution could spontaneously transform to hollow structure by incubating in hydrothermal condition. Meanwhile, the shell thickness of HMBGMs could be controlled by the hydrothermal time. In vitro drug release studies demonstrated that the HMBGMs exhibited a sustained release property. The obtained HMBGMs may have potential applications in drug delivery and bone tissue regeneration.