1. α-Lipoic Acid Exerts Its Antiviral Effect against Viral Hemorrhagic Septicemia Virus (VHSV) by Promoting Upregulation of Antiviral Genes and Suppressing VHSV-Induced Oxidative Stress
- Author
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Wangdong Li, Jia Kuntong, Wanwan Zhang, Fangzhao Yu, Li Fengquan, Xiaoqi Chen, and Meisheng Yi
- Subjects
Immunology ,Cyprinidae ,Biology ,α-Lipoic acid (LA) ,Antiviral Agents ,Virus ,Novirhabdovirus ,Fish Diseases ,IFN response ,Virology ,Viral hemorrhagic septicemia virus (VHSV) ,Animals ,Viability assay ,Antiviral ,Cells, Cultured ,Thioctic Acid ,Rhabdoviridae ,biology.organism_classification ,ISG15 ,Up-Regulation ,Oxidative Stress ,Viral replication ,Viperin ,Molecular Medicine ,IRF7 ,Viral hemorrhagic septicemia ,Research Article - Abstract
Viral hemorrhagic septicemia virus (VHSV), belonging to the genus Novirhabdovirus, Rhabdoviridae family, is a causative agent of high mortality in fish and has caused significant losses to the aquaculture industry. Currently, no effective vaccines, Food and Drug Administration-approved inhibitors, or other therapeutic intervention options are available against VHSV. α-Lipoic Acid (LA), a potent antioxidant, has been proposed to have antiviral effects against different viruses. In this study, LA (CC50 = 472.6 μmol/L) was repurposed to exhibit antiviral activity against VHSV. In fathead minnow cells, LA significantly increased the cell viability post-VHSV infection (EC50 = 42.7 μmol/L), and exerted a dose-dependent inhibitory effect on VHSV induced-plaque, cytopathic effects, and VHSV glycoprotein expression. The time-of-addition assay suggested that the antiviral activity of LA occurred at viral replication stage. Survival assay revealed that LA could significantly upregulated the survival rate of VHSV-infected largemouth bass in both co-injection (38.095% vs. 1.887%, P
- Published
- 2021