1. Identification and evaluation of universal epitopes in Plasmodium vivax Duffy binding protein
- Author
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Paola Martinez, Carolina Saravia, Manuel A. Patarroyo, Diana Granados, Claudia Reyes, and Carolina López
- Subjects
Unclassified drug ,Protein Conformation ,Hla dr4 antigen ,Enzyme linked immunosorbent assay ,Hla-dr antigens ,Plasmodium vivax ,Protozoan Proteins ,Universal epitopes ,Duffy binding protein ,Biochemistry ,Epitope ,Protein structure ,Malaria vaccines ,Sequence alignment ,Protein analysis ,Peptide sequence ,Priority journal ,Vaccines, Synthetic ,biology ,cell surface ,Protein conformation ,synthetic ,Biotinylation ,Hla dr1 antigen ,Protozoan proteins ,Synthetic vaccine ,Receptors, cell surface ,Molecular Sequence Data ,Epitope mapping ,Biophysics ,Antigens, Protozoan ,Receptors, Cell Surface ,Antigen binding ,Article ,Amino acid sequence ,Hla dr7 antigen ,Vaccines, synthetic ,Molecular sequence data ,Malaria Vaccines ,Animals ,Amino Acid Sequence ,Molecular Biology ,Immunodominant Epitopes ,protozoan ,Duffy binding protein (dbp) ,Binding protein ,HLA-DR Antigens ,Cell Biology ,biology.organism_classification ,Molecular biology ,Hla dr11 antigen ,Malaria ,Peptides ,Sequence Alignment ,Controlled study ,Nucleotide sequence ,Epitope Mapping ,Immunodominant epitopes ,HLA-DRB1 Chains - Abstract
Selected PvDBP-derived synthetic peptides were tested in competition assays with HLA molecules in order to identify and evaluate their binding to a wide range of MHC class II molecules. Binding was evaluated as the peptide's ability to displace the biotinylated control peptide (HA306-318) and was detected by a conventional ELISA. Thus, one epitope for the HLA-DR1 molecule, two epitopes for the HLA-DR4 molecule, six epitopes for the HLA-DR7 molecule and three epitopes for the HLA-DR11 molecule displaying a high binding percentage (above 50%) were experimentally obtained. The in vitro results were compared with the epitope prediction results. Two peptides behaved as universal epitopes since they bound to a larger number of HLA-DR molecules. Given that these peptides are located in the conserved PvDBP region II, they could be considered good candidates to be included in the design of a synthetic vaccine against Plasmodium vivax malaria. © 2008 Elsevier Inc. All rights reserved.
- Published
- 2008
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