42 results on '"Hitomi Nakamura"'
Search Results
2. Corrigendum to ‘An international genome-wide meta-analysis of primary biliary cholangitis: Novel risk loci and candidate drugs’ [J Hepatol 2021;75(3):572–581]
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Heather J. Cordell, James J. Fryett, Kazuko Ueno, Rebecca Darlay, Yoshihiro Aiba, Yuki Hitomi, Minae Kawashima, Nao Nishida, Seik-Soon Khor, Olivier Gervais, Yosuke Kawai, Masao Nagasaki, Katsushi Tokunaga, Ruqi Tang, Yongyong Shi, Zhiqiang Li, Brian D. Juran, Elizabeth J. Atkinson, Alessio Gerussi, Marco Carbone, Rosanna Asselta, Angela Cheung, Mariza de Andrade, Aris Baras, Julie Horowitz, Manuel A.R. Ferreira, Dylan Sun, David E. Jones, Steven Flack, Ann Spicer, Victoria L. Mulcahy, Jinyoung Byun, Younghun Han, Richard N. Sandford, Konstantinos N. Lazaridis, Christopher I. Amos, Gideon M. Hirschfield, Michael F. Seldin, Pietro Invernizzi, Katherine A. Siminovitch, Xiong Ma, Minoru Nakamura, George F. Mells, Andrew Mason, Catherine Vincent, Gang Xie, Jinyi Zhang, Andrea Affronti, Piero L. Almasio, Domenico Alvaro, Pietro Andreone, Angelo Andriulli, Francesco Azzaroli, Pier Maria Battezzati, Antonio Benedetti, Maria Consiglia Bragazzi, Maurizia Brunetto, Savino Bruno, Vincenza Calvaruso, Vincenzo Cardinale, Giovanni Casella, Nora Cazzagon, Antonio Ciaccio, Barbara Coco, Agostino Colli, Guido Colloredo, Massimo Colombo, Silvia Colombo, Laura Cristoferi, Carmela Cursaro, Lory Saveria Crocè, Andrea Crosignani, Daphne D’Amato, Francesca Donato, Gianfranco Elia, Luca Fabris, Stefano Fagiuoli, Carlo Ferrari, Annarosa Floreani, Andrea Galli, Edoardo Giannini, Ignazio Grattagliano, Pietro Lampertico, Ana Lleo, Federica Malinverno, Clara Mancuso, Fabio Marra, Marco Marzioni, Sara Massironi, Alberto Mattalia, Luca Miele, Chiara Milani, Lorenzo Morini, Filomena Morisco, Luigi Muratori, Paolo Muratori, Grazia A. Niro, Sarah O’Donnell, Antonio Picciotto, Piero Portincasa, Cristina Rigamonti, Vincenzo Ronca, Floriano Rosina, Giancarlo Spinzi, Mario Strazzabosco, Mirko Tarocchi, Claudio Tiribelli, Pierluigi Toniutto, Luca Valenti, Maria Vinci, Massimo Zuin, Hitomi Nakamura, Seigo Abiru, Shinya Nagaoka, Atsumasa Komori, Hiroshi Yatsuhashi, Hiromi Ishibashi, Masahiro Ito, Kiyoshi Migita, Hiromasa Ohira, Shinji Katsushima, Atsushi Naganuma, Kazuhiro Sugi, Tatsuji Komatsu, Tomohiko Mannami, Kouki Matsushita, Kaname Yoshizawa, Fujio Makita, Toshiki Nikami, Hideo Nishimura, Hiroshi Kouno, Hirotaka Kouno, Hajime Ota, Takuya Komura, Yoko Nakamura, Masaaki Shimada, Noboru Hirashima, Toshiki Komeda, Keisuke Ario, Makoto Nakamuta, Tsutomu Yamashita, Kiyoshi Furuta, Masahiro Kikuchi, Noriaki Naeshiro, Hironao Takahashi, Yutaka Mano, Seiji Tsunematsu, Iwao Yabuuchi, Yusuke Shimada, Kazuhiko Yamauchi, Rie Sugimoto, Hironori Sakai, Eiji Mita, Masaharu Koda, Satoru Tsuruta, Hiroshi Kamitsukasa, Takeaki Sato, Naohiko Masaki, Tatsuro Kobata, Nobuyoshi Fukushima, Yukio Ohara, Toyokichi Muro, Eiichi Takesaki, Hitoshi Takaki, Tetsuo Yamamoto, Michio Kato, Yuko Nagaoki, Shigeki Hayashi, Jinya Ishida, Yukio Watanabe, Masakazu Kobayashi, Michiaki Koga, Takeo Saoshiro, Michiyasu Yagura, Keisuke Hirata, Atsushu Tanaka, Hajime Takikawa, Mikio Zeniya, Masanori Abe, Morikazu Onji, Shuichi Kaneko, Masao Honda, Kuniaki Arai, Teruko Arinaga-Hino, Etsuko Hashimoto, Makiko Taniai, Takeji Umemura, Satoru Joshita, Kazuhiko Nakao, Tatsuki Ichikawa, Hidetaka Shibata, Satoshi Yamagiwa, Masataka Seike, Koichi Honda, Shotaro Sakisaka, Yasuaki Takeyama, Masaru Harada, Michio Senju, Osamu Yokosuka, Tatsuo Kanda, Yoshiyuki Ueno, Kentaro Kikuchi, Hirotoshi Ebinuma, Takashi Himoto, Michio Yasunami, Kazumoto Murata, Masashi Mizokami, Kazuhito Kawata, Shinji Shimoda, Yasuhiro Miyake, Akinobu Takaki, Kazuhide Yamamoto, Katsuji Hirano, Takafumi Ichida, Akio Ido, Hirohito Tsubouchi, Kazuaki Chayama, Kenichi Harada, Yasuni Nakanuma, Yoshihiko Maehara, Akinobu Taketomi, Ken Shirabe, Yuji Soejima, Akira Mori, Shintaro Yagi, Shinji Uemoto, Egawa H, Tomohiro Tanaka, Noriyo Yamashiki, Sumito Tamura, Yasuhiro Sugawara, Norihiro Kokudo, Naga Chalasani, Vel Luketic, Joseph Odin, Kapil Chopra, Goncalo Abecasis, Michael Cantor, Giovanni Coppola, Aris Economides, Luca A. Lotta, John D. Overton, Jeffrey G. Reid, Alan Shuldiner, Christina Beechert, Caitlin Forsythe, Erin D. Fuller, Zhenhua Gu, Michael Lattari, Alexander Lopez, Thomas D. Schleicher, Maria Sotiropoulos Padilla, Karina Toledo, Louis Widom, Sarah E. Wolf, Manasi Pradhan, Kia Manoochehri, Ricardo H. Ulloa, Xiaodong Bai, Suganthi Balasubramanian, Leland Barnard, Andrew Blumenfeld, Gisu Eom, Lukas Habegger, Alicia Hawes, Shareef Khalid, Evan K. Maxwell, William Salerno, Jeffrey C. Staples, Marcus B. Jones, Lyndon J. Mitnaul, Richard Sturgess, Christopher Healey, Andrew Yeoman, Anton V.J. Gunasekera, Paul Kooner, Kapil Kapur, V. Sathyanarayana, Yiannis Kallis, Javaid Subhani, Rory Harvey, Roger McCorry, Paul Rooney, David Ramanaden, Richard Evans, Thiriloganathan Mathialahan, Jaber Gasem, Christopher Shorrock, Mahesh Bhalme, Paul Southern, Jeremy A. Tibble, David A. Gorard, Susan Jones, George Mells, Victoria Mulcahy, Brijesh Srivastava, Matthew R. Foxton, Carole E. Collins, David Elphick, Mazn Karmo, Francisco Porras-Perez, Michael Mendall, Tom Yapp, Minesh Patel, Roland Ede, Joanne Sayer, James Jupp, Neil Fisher, Martyn J. Carter, Konrad Koss, Jayshri Shah, Andrzej Piotrowicz, Glyn Scott, Charles Grimley, Ian R. Gooding, Simon Williams, Judith Tidbury, Guan Lim, Kuldeep Cheent, Sass Levi, Dina Mansour, Matilda Beckley, Coral Hollywood, Terry Wong, Richard Marley, John Ramage, Harriet M. Gordon, Jo Ridpath, Theodore Ngatchu, Vijay Paul Bob Grover, Ray G. Shidrawi, George Abouda, L. Corless, Mark Narain, Ian Rees, Ashley Brown, Simon Taylor-Robinson, Joy Wilkins, Leonie Grellier, Paul Banim, Debasish Das, Michael A. Heneghan, Howard Curtis, Helen C. Matthews, Faiyaz Mohammed, Mark Aldersley, Raj Srirajaskanthan, Giles Walker, Alistair McNair, Amar Sharif, Sambit Sen, George Bird, Martin I. Prince, Geeta Prasad, Paul Kitchen, Adrian Barnardo, Chirag Oza, Nurani N. Sivaramakrishnan, Prakash Gupta, Amir Shah, Chris D.J. Evans, Subrata Saha, Katharine Pollock, Peter Bramley, Ashis Mukhopadhya, Stephen T. Barclay, Natasha McDonald, Andrew J. Bathgate, Kelvin Palmer, John F. Dillon, Simon M. Rushbrook, Robert Przemioslo, Chris McDonald, Andrew Millar, Cheh Tai, Stephen Mitchell, Jane Metcalf, Syed Shaukat, Mary Ninkovic, Udi Shmueli, Andrew Davis, Asifabbas Naqvi, Tom J.W. Lee, Stephen Ryder, Jane Collier, Howard Klass, Matthew E. Cramp, Nichols Sharer, Richard Aspinall, Deb Ghosh, Andrew C. Douds, Jonathan Booth, Earl Williams, Hyder Hussaini, John Christie, Steven Mann, Douglas Thorburn, Aileen Marshall, Imran Patanwala, Aftab Ala, Julia Maltby, Ray Matthew, Chris Corbett, Sam Vyas, Saket Singhal, Dermot Gleeson, Sharat Misra, Jeff Butterworth, Keith George, Tim Harding, Andrew Douglass, Harriet Mitchison, Simon Panter, Jeremy Shearman, Gary Bray, Michael Roberts, Graham Butcher, Daniel Forton, Zahid Mahmood, Matthew Cowan, Debashis Das, Chin Lye Ch'ng, Mesbah Rahman, Gregory C.A. Whatley, Emma Wesley, Aditya Mandal, Sanjiv Jain, Stephen P. Pereira, Mark Wright, Palak Trivedi, Fiona H. Gordon, Esther Unitt, Altaf Palejwala, Andrew Austin, Vishwaraj Vemala, Allister Grant, Andrew D. Higham, Alison Brind, Ray Mathew, Mark Cox, Subramaniam Ramakrishnan, Alistair King, Simon Whalley, Jocelyn Fraser, S.J. Thomson, Andrew Bell, Voi Shim Wong, Richard Kia, Ian Gee, Richard Keld, Rupert Ransford, James Gotto, and Charles Millson
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Science & Technology ,Hepatology ,Gastroenterology & Hepatology ,Italian PBC Study Group ,Japan-PBC-GWAS Consortium ,UK-PBC Consortium ,Chinese PBC Consortium ,1103 Clinical Sciences ,US PBC Consortium ,Canadian PBC Consortium ,Life Sciences & Biomedicine ,PBC Consortia ,1117 Public Health and Health Services - Published
- 2021
3. Essential processes involving REE-enrichment in biogenic apatite in deep-sea sediment decoded via multivariate statistical analyses
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Kazutaka Yasukawa, Junichiro Ohta, Morihisa Hamada, Qing Chang, Hitomi Nakamura, Kana Ashida, Yutaro Takaya, Kentaro Nakamura, Hikaru Iwamori, and Yasuhiro Kato
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Geochemistry and Petrology ,Geology - Published
- 2022
4. Geochemical mapping of slab-derived fluid and source mantle along Japan arcs
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Takashi Miyazaki, Hikaru Iwamori, Qing Chang, Toshiro Takahashi, Jun-Ichi Kimura, Bogdan Stefanov Vaglarov, Tomoyuki Shibata, Hitomi Nakamura, Yuka Hirahara, and Mitsuhiro Nakagawa
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010504 meteorology & atmospheric sciences ,Subduction ,Mantle wedge ,Continental crust ,Geology ,Crust ,010502 geochemistry & geophysics ,01 natural sciences ,Mantle (geology) ,Plume ,Slab ,Metasomatism ,Petrology ,0105 earth and related environmental sciences - Abstract
Although slab-derived fluid significantly affects melt generation and dynamics within subduction zones, its amount and distribution are not sufficiently constrained at present. Herein, we use isotopic systematics of arc volcanic rocks, subducting materials, and intrinsic mantle components prior to metasomatism, to quantify the contribution of the slab-derived fluid that metasomatizes the overlying mantle wedge beneath the entire area of Japan arcs. Simultaneous application of several multivariate statistical analyses (clustering analysis and principal/independent component analyses) to the isotopic data set allows Japan arcs to be broadly divided into eastern and western parts at the first order. Moreover, a clear higher-order inter-arc segmentation is observed, together with some intra-arc variations that possibly correspond to the heterogeneity of incoming plates. Inter-arc segmentation is shown to be primarily controlled by the geometrical parameters of the slab and the arc (e.g., subduction of a single plate or double plates beneath either oceanic or continental crust), which results in differences between mantle wedge and slab thermal conditions. Accordingly, the Kuril and Izu arcs, which have thin arc crusts (~20 km), exhibit the lowest extent of slab-derived fluid addition (0.1 wt%) to the mantle wedge, while the NE Japan arc, with a thicker arc crust (up to 36 km), features a higher value of 0.2 wt%, although the slab thermal parameters for these three arcs are essentially the same. The Central Japan arc shows the highest extent of slab-derived fluid addition (>1.0 wt%) because of the overlapping subduction of Pacific and Philippine Sea slabs, while the SW Japan and Ryukyu arcs feature moderate values of ~0.5 wt%. Moreover, a clear exotic plume zone and spots are observed in SW Japan and the Japan Sea. In addition to the variability of slab-derived fluid composition, the intrinsic mantle composition (before slab-derived fluid–induced metasomatism) shows a clear along-arc variation that is possibly caused by a large-scale mantle flow from the continental side. Thus, slab-derived fluid addition and mantle composition variability equally contribute to inter-arc segmentation, which highlights the importance of both local and regional thermal flow structures of slab-mantle systems.
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- 2019
5. Trace-element characteristics of east–west mantle geochemical hemispheres
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Satoru Haraguchi, Atsushi Nakao, Kenta Ueki, Hikaru Iwamori, Takashi Nakagawa, Masaki Yoshida, Tatsuji Nishizawa, and Hitomi Nakamura
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Western hemisphere ,Basalt ,Global and Planetary Change ,010504 meteorology & atmospheric sciences ,Subduction ,East west ,Trace element ,Geochemistry ,010502 geochemistry & geophysics ,01 natural sciences ,Supercontinent ,Mantle (geology) ,General Earth and Planetary Sciences ,Eastern Hemisphere ,Geology ,0105 earth and related environmental sciences - Abstract
Recent statistical analyses on the isotopic compositions of oceanic, arc, and continental basalts have revealed that the Earth's mantle is broadly divided into eastern and western hemispheres. The present study aimed to characterize the isotopically defined east–west geochemical hemispheres using trace-element concentrations. Basalt data with Rb, Sr, Nd, Sm, Pb, Th, and U in addition to the isotopic ratios 87Sr/86Sr, 143Nd/144Nd, 206Pb/204Pb, 207Pb/204Pb, and 208Pb/204Pb were selected mostly from the GEOROC and PetDB databases. A total of 4787 samples were used to investigate the global geochemical variations. The results show that the wide trace-element variations are broadly explained by the melting of melt-metasomatized and fluid-metasomatized mantle sources. The larger amount of the fluid component derived from subducted plates in the eastern hemisphere than that in the western hemisphere is inferred from the basalts. These characteristics support the hypothesis that focused subduction towards the supercontinent created the mantle geochemical hemispheres.
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- 2019
6. Are naïve T cells and class-switched memory (IgD− CD27+) B cells not essential for establishment and maintenance of pregnancy? Insights from a case of common variable immunodeficiency with pregnancy
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Tadashi Kimura, Kohsuke Imai, Naoya Shigeta, Keiichi Kumasawa, Shimon Sakaguchi, Hitomi Nakamura, and Shigeru Saito
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0301 basic medicine ,Pregnancy ,Naive T cell ,business.industry ,Common variable immunodeficiency ,General Medicine ,Acquired immune system ,medicine.disease ,Hypogammaglobulinemia ,03 medical and health sciences ,030104 developmental biology ,Immune system ,medicine.anatomical_structure ,Immunology ,medicine ,Primary immunodeficiency ,business ,B cell - Abstract
The disruption of adaptive immune response has adverse effects on the establishment and maintenance of pregnancy. The adaptive immune system is regulated by several types of immune cells. However, there is limited information about cell hierarchy in the adaptive immune response to the establishment and maintenance of pregnancy in women. The assessment of the outcome of pregnancy in primary immunodeficiency diseases could help in understanding the cell hierarchy in the adaptive immune system during pregnancy. Common variable immunodeficiency (CVID) is a heterogeneous adaptive immune system disorder characterized by primary hypogammaglobulinemia. A few studies have previously reported the assessment of the T and B cell subpopulations in CVID patients. However, an assessment of the subpopulations of T and B cells and the outcome of pregnancy in women with CVID has not been reported till date. Most CVID patients show a general decrease in the expression of CD27 in B cells. The assessment of pregnancy and the subpopulations of T and B cells in CVID women with severe reduction in the naive T and switched B cells could help understand whether these cells are essential for the establishment and maintenance of pregnancy in women.
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- 2018
7. Distribution of slab-derived fluids around the edge of the Philippine Sea Plate from Central to Northeast Japan
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Osamu Ishizuka, Hikaru Iwamori, Tatsuji Nishizawa, and Hitomi Nakamura
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geography ,geography.geographical_feature_category ,010504 meteorology & atmospheric sciences ,Mantle wedge ,Subduction ,Geodynamics ,010502 geochemistry & geophysics ,01 natural sciences ,Mantle (geology) ,Volcanic rock ,Geophysics ,Magmatism ,Transition zone ,Slab ,Petrology ,Geology ,0105 earth and related environmental sciences ,Earth-Surface Processes - Abstract
Marginal parts of a plate and subducting slab can play important roles in geodynamics. This is because in areas where a plate interacts with other plates or with the mantle thermal, geochemical, and mechanical interactions are expected. The Philippine Sea (PHS) slab that subducts beneath the Japan arcs has such an edge. To examine the relationship between arc magmatism and the slab edge in the transition zone from Northeast Japan to Central Japan, we investigated isotopic systematics of the regional volcanic rocks, incorporating data from literature and new data for five isotopic ratios of Sr, Nd, and Pb. The new data included major element compositions of 22 samples from the back-arc area, and 5 isotopic ratios for 6 samples selected from Pleistocene to early Quaternary epochs. Consequently, several findings were determined based on the spatial variation of the isotopic ratios and the estimated amount of slab-derived fluid: (1) the amount of fluid derived from the two subducting slabs (i.e., the Pacific slab and the PHS slab) decreases northward from a significantly high value (~ 5 wt% fluid added to the source mantle), away from the seismically determined edge of the PHS slab; (2) the proportion of the PHS component in the total slab-derived fluid also decays northward; and (3) the PHS component spreads to the north beyond the seismically determined edge of the PHS slab. These observations strongly suggest that the existence of an aseismic PHS slab beneath southernmost parts of Northeast Japan delivers the PHS component to the arc magmatism. As was indicated by previous geodynamical studies, subduction of the PHS and PAC slabs may generate suction force towards the corner of mantle wedge, which might account for the large amount of fluid near the seismically determined slab edge as described in (1) above.
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- 2018
8. A novel engineered interchain disulfide bond in the constant region enhances the thermostability of adalimumab Fab
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Hitomi Nakamura, Tadashi Ueda, Takatoshi Ohkuri, and Naoko Oda-Ueda
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Models, Molecular ,0301 basic medicine ,Protein Conformation ,Stereochemistry ,Biophysics ,macromolecular substances ,Protein Engineering ,Biochemistry ,Pichia ,Pichia pastoris ,Immunoglobulin Fab Fragments ,03 medical and health sciences ,Humans ,Disulfides ,Molecular Biology ,Thermostability ,Constant region ,biology ,Protein Stability ,Tumor Necrosis Factor-alpha ,Chemistry ,Adalimumab ,Disulfide bond ,Cell Biology ,biology.organism_classification ,Recombinant Proteins ,030104 developmental biology ,Yield (chemistry) ,PEGylation ,Thermodynamics ,Conformational stability - Abstract
We constructed a system for expressing the Fab of the therapeutic human monoclonal antibody adalimumab at a yield of 20 mg/L in the methylotrophic yeast Pichia pastoris. To examine the contribution of interchain disulfide bonds to conformational stability, we prepared adalimumab Fab from which the interchain disulfide bond at the C-terminal region at both the CH1 and CL domains was deleted by substitution of Cys with Ala (FabΔSS). DSC measurements showed that the Tm values of FabΔSS were approximately 5 °C lower than those of wild-type Fab, suggesting that the interchain disulfide bond contributes to conformational thermostability. Using computer simulations, we designed a novel interchain disulfide bond outside the C-terminal region to increase the stability of FabΔSS. The resulting Fab (mutSS FabΔSS) had the mutations H:V177C and L:Q160C in FabΔSS, confirming the formation of the disulfide bond between CH1 and CL. The thermostability of mutSS FabΔSS was approximately 5 °C higher than that of FabΔSS. Therefore, the introduction of the designed interchain disulfide bond enhanced the thermostability of FabΔSS and mitigated the destabilization caused by partial reduction of the interchain disulfide bond at the C-terminal region, which occurs in site-specific modification such as PEGylation.
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- 2018
9. P-003. Complement activation by an angiogenic imbalance leads to systemic vascular endothelial dysfunction
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Tatsuya Matsuyama, Hitomi Nakamura, Masayuki Endo, Kazunobu Yagi, Kazuya Mimura, Takuji Tomimatsu, Reina Komatsu, Tadashi Kimura, and Yoko Kawanishi
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business.industry ,Internal Medicine ,medicine ,Cancer research ,Obstetrics and Gynecology ,Endothelial dysfunction ,medicine.disease ,business ,Complement system - Published
- 2021
10. P-050. Potency of Tokishakuyakusan in treating preeclampsia: Drug repositioning method by in vitro screening of the Kampo library
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Tadashi Kimura, Kazunobu Yagi, Yoko Kawanishi, Takuji Tomimatsu, Kazuya Mimura, Hitomi Nakamura, Tatsuya Matsuyama, and Masayuki Endo
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Drug repositioning ,Tokishakuyakusan ,business.industry ,Kampo ,Internal Medicine ,medicine ,Obstetrics and Gynecology ,Potency ,Pharmacology ,medicine.disease ,business ,In vitro ,Preeclampsia - Published
- 2021
11. Effect of lipid metabolism on male fertility
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Keiichi Kumasawa, Tadashi Kimura, Hitomi Nakamura, Namhyo Kim, Ken-ichi Hirano, and Hidetake Masaki
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Blood Glucose ,Male ,0301 basic medicine ,medicine.medical_specialty ,Very low-density lipoprotein ,Hypercholesterolemia ,Biophysics ,Lipoproteins, VLDL ,Biology ,Asthenozoospermia ,Biochemistry ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Chylomicrons ,medicine ,Animals ,Humans ,Lipolysis ,Obesity ,Spermatogenesis ,Molecular Biology ,Infertility, Male ,Triglycerides ,Sperm motility ,Epididymis ,Mice, Knockout ,030219 obstetrics & reproductive medicine ,Hypertriglyceridemia ,Lipid metabolism ,Cell Biology ,Lipid Metabolism ,medicine.disease ,Sperm ,Mice, Inbred C57BL ,Cholesterol ,Fertility ,030104 developmental biology ,Endocrinology ,Diabetes Mellitus, Type 2 ,Hyperglycemia ,Sperm Motility ,Receptors, Leptin ,lipids (amino acids, peptides, and proteins) - Abstract
Cholesterol and lipid homeostasis is important for male fecundity. However, the plasma total cholesterol level does not reflect sperm concentration and motility. Adipose tissue in mammals is the main tissue contributing to the dynamic equilibrium of lipid synthesis and catabolism. However, recent studies suggested that local lipolysis has an important role in male fertility. If so, which plasma lipid metabolism parameter reflects sperm concentration and motility? Leptin receptor-deficient db/db mice show hyperphagia, hyperglycemia, hypercholesterolemia, hypertriglyceridemia, morbid obesity and are reported to be infertile. Impairment of spermatogenesis and sperm motility in db/db mice was observed in our experiments. Medium-chain triglycerides (MCTs) are more quickly metabolized as fuel compared to long-chain triglycerides (LCTs). If the LCTs are replaced with MCTs in the diet, even in hyperphagia, does it affect spermatogenesis and sperm motility? In this study, we investigated the effect of a MCT replacement diet on sperm parameters using db/db mice. Six weeks of MCT replacement diet improved not only spermatogenesis but also the maturation processes in the epididymis for sperm to acquire the ability to move forward. Plasma chylomicron and large VLDL levels showed positive correlation with total and motile sperm concentrations. The MCT replacement diet could be an effective treatment for idiopathic non-obstructive oligozoospermia or asthenozoospermia men with low levels of chylomicron and large VLDL.
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- 2017
12. Prevalence of obstetric complications including placenta abnormality in pregnancies achieved by oocyte donation in our institution
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Tadashi Kimura, Naoko Takahashi, Tsuyoshi Takiuchi, Sachi Takaoka, Mika Handa, Hitomi Nakamura, Tatsuya Miyake, Masako Kanda, and Futa Ito
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medicine.medical_specialty ,medicine.anatomical_structure ,Reproductive Medicine ,business.industry ,Obstetrics ,Placenta ,Oocyte donation ,medicine ,Obstetrics and Gynecology ,Abnormality ,business ,Developmental Biology - Published
- 2019
13. Effect of the 3'-sulfo-Lewis a and 3'-sulfo-Lewis x epitopes remodelling on mouse placentation
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John D. Aplin, Tadashi Kimura, Hitomi Nakamura, Carolyn J.P. Jones, Takuji Tomimatsu, Kazuya Mimura, and Kazunobu Yagi
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3-sulfo Lewis x ,Reproductive Medicine ,Chemistry ,Stereochemistry ,Obstetrics and Gynecology ,Placentation ,Epitope ,Developmental Biology - Published
- 2021
14. Franck-Condon relaxation in photo-excited YAG:Ce studied using real-time time-dependent density functional theory
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Hitomi Nakamura, Yoshiyuki Miyamoto, and Tomoko Akai
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Materials science ,Biophysics ,chemistry.chemical_element ,02 engineering and technology ,General Chemistry ,Yttrium ,Time-dependent density functional theory ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,01 natural sciences ,Biochemistry ,Atomic and Molecular Physics, and Optics ,0104 chemical sciences ,Bond length ,Photoexcitation ,symbols.namesake ,chemistry ,Excited state ,Stokes shift ,symbols ,Light emission ,Density functional theory ,Atomic physics ,0210 nano-technology - Abstract
First-principles calculations of electron-ion dynamics based on real-time time-dependent density functional theory were performed to study the lattice dynamics in yttrium aluminum garnet (YAG) doped with cerium (Ce) following photoexcitation. The dynamics induced by the optical transitions from the valence band to the conduction band of the YAG host and from the Ce 4f to 5 d levels were examined. Significant lattice motion was observed in the latter case. This lattice motion (Franck-Condon relaxation) decreased the Ce–O bond length by 0.05 A and increased the energy of the Ce 4f level by 0.5 eV, suggesting a Stokes shift in the light emission due to the Ce 5 d→4f transition. The calculated Stokes shift is consistent with the recently reported experimental value, despite the approximations used in the present calculations. The effect of lattice temperature was also examined up to 300 K.
- Published
- 2021
15. Relationship between the magnitude of IgE production in mice and conformational stability of the house dust mite allergen, Der p 2
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Takanori So, Takatoshi Ohkuri, Tadashi Ueda, and Hitomi Nakamura
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0301 basic medicine ,Protein Denaturation ,T-Lymphocytes ,Antigen presentation ,Molecular Conformation ,Biophysics ,Antigen-Presenting Cells ,medicine.disease_cause ,Immunoglobulin E ,Major histocompatibility complex ,Biochemistry ,Arthropod Proteins ,Mice ,03 medical and health sciences ,Allergen ,Antigen ,medicine ,Animals ,Humans ,Denaturation (biochemistry) ,Antigens, Dermatophagoides ,Antigen-presenting cell ,Molecular Biology ,030102 biochemistry & molecular biology ,biology ,Chemistry ,Immunogenicity ,Pyroglyphidae ,Molecular biology ,030104 developmental biology ,Immunology ,biology.protein ,Immunization - Abstract
Background Protein antigens are degraded by endosomal protease in antigen presentation cell. T cells recognize peptides derived from antigen proteins bound to class II major histocompatibility complex molecules. We previously reported that an increase in the conformational stability of an antigen depressed its immunogenicity. However, there is little information on antigens with differences in molecular properties such as net charges and molecular weight. Methods Denaturation experiments against guanidine hydrochloride. The serum IgE levels to protein antigens at 35 days after the first immunization analyzed using ELISA. Results The Der p 2 mutations in which Ile13 is mutated to Ala (I13A) and Ala122 is mutated to Ile (A122I) were shown to have lower and higher conformational stability than the wild-type, respectively, by denaturation experiments. The amount of IgE production by the less stable I13A mutant was higher and that of the stable A122I mutant was lower than that of the wild-type. Conclusion Our results suggest that the increased conformational stability of Der p 2 depressed the IgE production in mice. General significance These findings should provide a milestone for the engineering of allergen vaccines.
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- 2016
16. Compositional heterogeneity of Archean mantle estimated from Sr and Nd isotopic systematics of basaltic rocks from North Pole, Australia, and the Isua supracrustal belt, Greenland
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Hikaru Iwamori, Saya Kagami, Tetsuya Yokoyama, Tsuyoshi Komiya, Ayane Sano, Hitomi Nakamura, and Akira Ishikawa
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Isochron ,Basalt ,010504 meteorology & atmospheric sciences ,Pilbara Craton ,Archean ,Geochemistry ,Trace element ,Metamorphism ,Geology ,Ocean island basalt ,010502 geochemistry & geophysics ,01 natural sciences ,Mantle (geology) ,Geochemistry and Petrology ,0105 earth and related environmental sciences - Abstract
Compositional variability found in modern mid-ocean ridge basalt (MORB) and ocean island basalt (OIB) reflects differentiation processes associated with material recycling in the mantle-crust system. To investigate the timing at which this recycling system was established and how it transformed into the present-day system, we present geochemical analyses of the Archean basalts from North Pole (NP) in the East Pilbara Craton, Western Australia, and the Isua supracrustal belt (ISB), southern West Greenland. These rocks represent Archean accretionary complexes with ages of ~3.5 Ga and 3.7–3.8 Ga, respectively. We analyzed the trace element contents including rare earth elements (REEs), and Sr and Nd isotopic compositions of the basalts, which may represent MORBs and OIBs, from NP and ISB. Their trace-element compositions are broadly similar, but show distinct geochemical characteristics particularly with respect to REEs that probably reflect differences in both the source mantle and degree of melting. Such differences are also evident in their initial Nd isotopic compositions, which were estimated based on equilibrium partitioning of REEs and well-defined isochron ages. In contrast, the Sr isotopic compositions of the NP and ISB basalts are highly variable and their isochron ages are inconsistent with previous studies. Furthermore, the partitioning of Rb and Sr in the NP basalts indicates disequilibrium, suggesting that the Rb-Sr system has been disturbed by post-igneous alteration and metamorphism. Based on these observations, we propose the following model to explain the temporal variations in the geochemical composition of the Archean mantle: (i) ~3800 Ma: recycling of plate material and melting occurred quite readily and, therefore, MORBs and OIBs were produced from differentiated mantle sources; (ii) 3460 Ma to ~3800 Ma: mantle-crust mixing occurred as the result of an extreme event, such as mantle overturning, reducing the compositional variation of the mantle; and (iii) after ~3460 Ma: mantle heterogeneity gradually developed in the material-recycling system, re-establishing the compositional differences between MORBs and OIBs. This model requires an extreme event to drive the homogenization during stage (ii), which may provide new insights into the evolution of the crust-mantle system.
- Published
- 2020
17. Su1440 THE RISK FACTORS OF DELAYED HEMORRHAGE AFTER ENDOSCOPIC SPHINCTEROTOMY IN OUR CASES
- Author
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Akiko Matsuda, Yoshiyuki Ueno, Yasuharu Kakizaki, Toshikazu Kobayashi, Shimpei Sugahara, Tetsuya Ishizawa, Michihiko Tsunoda, Naohiko Makino, Chisaki Ikeda, and Hitomi Nakamura
- Subjects
medicine.medical_specialty ,business.industry ,Gastroenterology ,Medicine ,Radiology, Nuclear Medicine and imaging ,business ,Surgery - Published
- 2020
18. Microfibers from interpolymer complexation of κ-carrageenan and oligomers of polyaniline for glucose detection
- Author
-
Chunye Xu, Hitomi Nakamura, Hongshu Zhu, Dan Zhu, and Masaru Matsuo
- Subjects
chemistry.chemical_classification ,Conductive polymer ,Materials science ,Mechanical Engineering ,Metals and Alloys ,Polymer ,Sulfonic acid ,Condensed Matter Physics ,Electronic, Optical and Magnetic Materials ,chemistry.chemical_compound ,Aniline ,chemistry ,Polymerization ,Mechanics of Materials ,Polymer chemistry ,Polyaniline ,Materials Chemistry ,Self-assembly ,In situ polymerization - Abstract
The molecular order and orientation significantly influence the physical properties of conjugated polymers and the corresponding performance in electronic devices. The precise synthesis and characterization are the challenges to obtain high quality polymer crystalline micro/nanostructures, on the basis of which, the charge transport mechanisms can be discussed and the device performance can be improved. A micro-fibrous complex of conductive polymer and natural polysaccharide has been formed during the in situ polymerization of aniline in the aqueous solution of κ-carrageenan. With the template of the polyanion, aniline is polymerized and polyaniline (PANI) chain grows in the vicinity of sulfonic acid groups. Through the comprehensive characterizations of UV–vis spectra, wide-angle X-ray diffraction, and thermal gravimetric analysis, the assembly structure of the interpolymer complex has been illuminated, i.e., oligomers of PANI with expanded conformation are formed, the electrostatic interaction between the protonated PANI oligomers and anionic κ-carrageenan makes the chains of PANI and κ-carrageenan bound together, and a micro-fibrous complex is formed with enhanced orientation of both PANI and κ-carrageenan, leading to an enhanced conductivity of the complex. The glucose biosensor made of the complex shows high detecting sensitivity and broad linear range in the recognition of the glucose due to its high conductivity and good compatibility with the enzyme.
- Published
- 2015
19. Activity of formaldehyde dehydrogenase on titanium dioxide films with different crystallinities
- Author
-
Hitomi Nakamura, Kazumi Kato, Katsuya Kato, and Yoshitake Masuda
- Subjects
chemistry.chemical_classification ,Materials science ,Immobilized enzyme ,biology ,Inorganic chemistry ,General Physics and Astronomy ,Surfaces and Interfaces ,General Chemistry ,Condensed Matter Physics ,Enzyme assay ,Surfaces, Coatings and Films ,Crystallinity ,chemistry.chemical_compound ,Adsorption ,Enzyme ,chemistry ,Chemical engineering ,Titanium dioxide ,biology.protein ,Biosensor ,Formaldehyde dehydrogenase - Abstract
Many biosensors have been developed and used in recent years, and to enhance the sensitivity and stability of enzyme biosensors, immobilization of the enzymes on material surfaces is a necessary and important step. Therefore, there has been considerable interest in understanding how material interfaces affect enzyme adsorption. In this study, the influence of the crystallinity of titanium dioxide (TiO2) films on the quantity and activity of the immobilized enzyme, i.e., formaldehyde dehydrogenase (FDH), was investigated. It was found that TiO2 films with high crystallinity, which were annealed at 550 °C, showed higher enzyme immobilization and activity compared with the non-annealed TiO2 film. These results suggest that the activity of enzymes could be affected by the crystallinity of surface materials.
- Published
- 2015
20. Epithelium specific ETS transcription factor, ESE-3, of Protobothrops flavoviridis snake venom gland transactivates the promoters of venom phospholipase A2 isozyme genes
- Author
-
Takahito Chijiwa, Yoshiyuki Sakaki, Hitomi Nakamura, Shosaku Hattori, Tatsuo Murakami, Takatoshi Ohkuri, Naoko Oda-Ueda, and Motonori Ohno
- Subjects
DNA, Complementary ,TATA box ,Genetic Vectors ,Molecular Sequence Data ,Electrophoretic Mobility Shift Assay ,Venom ,CHO Cells ,Toxicology ,complex mixtures ,Cricetulus ,Exocrine Glands ,Japan ,Viperidae ,Cricetinae ,biology.animal ,Crotalid Venoms ,Animals ,Luciferases ,Promoter Regions, Genetic ,Transcription factor ,DNA Primers ,Expressed Sequence Tags ,Base Sequence ,Proto-Oncogene Proteins c-ets ,biology ,Reverse Transcriptase Polymerase Chain Reaction ,ETS transcription factor family ,Promoter ,Sequence Analysis, DNA ,Venom Protein ,Molecular biology ,Enzyme Activation ,Isoenzymes ,Phospholipases A2 ,Snake venom ,Fluorescein-5-isothiocyanate - Abstract
Protobothrops flavoviridis (habu) (Crotalinae, Viperidae) is a Japanese venomous snake, and its venom contains the enzymes with a variety of physiological activities. The phospholipases A2 (PLA2s) are the major components and exert various toxic effects. They are expressed abundantly in the venom gland. It is thought that the venom gland-specific transcription factors play a key role for activation of PLA2 genes specifically expressed in the venom gland. Thus, the full-length cDNA library for P. flavoviridis venom gland after milking of the venom was made to explore the transcription factors therein. As a result, three cDNAs encoding epithelium-specific ETS transcription factors (ESE)-1, -2, and -3 were obtained. Among them, ESE-3 was specifically expressed in the venom gland and activated the proximal promoters of venom PLA2 genes, which are possibly regarded as the representatives of the venom gland-specific protein genes in P. flavoviridis. Interestingly, the binding specificity of ESE-3 to the ETS binding motif located near TATA box is well correlated with transcriptional activities for the venom PLA2 genes. This is the first report that venom gland-specific transcription factor could actually activate the promoters of the venom protein genes.
- Published
- 2014
21. DNA interaction with dipolar ruthenium(II) ammine complexes containing 4,4′-bipyridinium as photochemotherapeutic agents
- Author
-
Yasuo Nakabayashi, Yuya Kubota, Misaki Nakai, Tomotaka Kawasaki, Hitomi Nakamura, Mika Morimoto, and Osamu Yamauchi
- Subjects
Hydrogen ,Chemistry ,Stereochemistry ,Photodissociation ,Intercalation (chemistry) ,Stacking ,chemistry.chemical_element ,Electrochemistry ,Ruthenium ,Inorganic Chemistry ,Crystallography ,chemistry.chemical_compound ,Materials Chemistry ,Physical and Theoretical Chemistry ,DNA ,Visible spectrum - Abstract
The interaction of DNA with dipolar ruthenium(II) ammine complexes containing 4,4′-bipyridinium cations L + , [Ru(L + )(NH 3 ) 5 ] 3+ , has been investigated by spectroscopic and electrochemical methods. These ruthenium(II) complexes undergo strong stacking interactions of L + with the base-pairs of DNA duplex in addition to the electrostatic and hydrogen binding modes. The phbp + ([ 2 ] 3+ ) and pymbp + ([ 3 ] 3+ ) complexes bind to DNA more strongly than the mebp + ([ 1 ] 3+ ) complex, due to the presence of extended planar aromatic ligands. [ 3 ] 3+ which exhibited the strongest interaction with DNA can bind to supercoiled plasmid pBR322 DNA following photoinduced loss of ligands pymbp + and NH 3 . The finding led to the photocleavage of supercoiled plasmid pBR322 DNA by [ 3 ] 3+ with visible light under aerobic and unaerobic conditions. Since similar photolysis of [ 1 ] 3+ and [ 2 ] 3+ has been observed, all the dipolar ruthenium(II) ammine complexes bearing 4,4′-bipyridinium cations can function as O 2 -independent photochemotherapeutic agents.
- Published
- 2014
22. Enzyme encapsulation in silica gel prepared by polylysine and its catalytic activity
- Author
-
Katsuya Kato, Yuki Kawachi, Shin-ichi Kugimiya, and Hitomi Nakamura
- Subjects
Chromatography ,biology ,Silica gel ,Lysine ,General Physics and Astronomy ,Surfaces and Interfaces ,General Chemistry ,Condensed Matter Physics ,Enzyme assay ,Surfaces, Coatings and Films ,Catalysis ,Hydrolysis ,chemistry.chemical_compound ,chemistry ,Polylysine ,biology.protein ,Enzyme kinetics ,Lipase - Abstract
Enzymes used in industrial applications are often immobilized onto different types of supports because they are sensitive to pH, temperature, and various other environmental conditions. However, many of the current immobilization approaches face problems such as the requirement of tedious multi-step procedures, loss of enzyme activity during immobilization, and poor reusability. In this study, we chose poly- l -lysine (Ki) as a catalyst for silica mineralization and attempted a one-step “leave to stand” synthesis method under mild conditions, so as to simultaneously maintain both high enzymatic activity and reusability. To examine the effect of Kx on the enzymatic reaction of lipase, we performed hydrolysis of 2-octylacetate without adding a silica precursor. Results indicate that Kx hardly exerts adverse influence on the enzymatic activity of lipase. The lipase encapsulated in the silica gel prepared by leave to stand (Gelstand) retained 70% of the activity compared to the free solution, which is two times higher than that obtained by mixing (Gelmix). However, the Km value was found to be similar to that of free enzymes. These results suggest that the leave to stand is a suitable procedure for immobilization, without any decrease in the mass transfer of substrate. The Gel-stand sample retained 100% activity even after the 5th cycle, and retained above 95% of its activity after 4 h of heat treatment at 65 °C. Using phenyltriethoxysilane as a silica precursor, tertiary structural stability of enzyme was obtained, and its Kcat value was improved when compared to a free solution.
- Published
- 2014
23. Discovery of a novel vascular endothelial growth factor (VEGF) with no affinity to heparin in Gloydius tsushimaensis venom
- Author
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Michihisa Toriba, Naoko Oda-Ueda, Motonori Ohno, Hitomi Nakamura, Tatsuo Murakami, Takahito Chijiwa, and Takahisa Imamura
- Subjects
Vascular Endothelial Growth Factor A ,Gloydius tsushimaensis ,Guinea Pigs ,Molecular Sequence Data ,Venom ,Vascular permeability ,Viper Venoms ,Toxicology ,complex mixtures ,Capillary Permeability ,chemistry.chemical_compound ,Japan ,Viperidae ,medicine ,Animals ,Amino Acid Sequence ,Receptor ,Peptide sequence ,Phylogeny ,Base Sequence ,biology ,Heparin ,biology.organism_classification ,Molecular biology ,Vascular endothelial growth factor ,chemistry ,Snake venom ,Electrophoresis, Polyacrylamide Gel ,Sequence Alignment ,medicine.drug - Abstract
Strong vascular permeability enhancing activity was found only in the venom of Gloydius tsushimaensis , in Tsushima island, Japan, when examined together with the venoms of G. blomhoffii snakes in several areas of Japan and of G. ussuriensis in South Korea. The active protein purified by using Superdex 75 and Mono Q columns showed no affinity to heparin, and migrated on SDS-PAGE with molecular weights of 26 and 13 kDa under nonreducing and reducing conditions, respectively, showing that it exists as homodimer. Its N-terminal amino acid sequence was highly homologous to those of snake venom vascular endothelial growth factors (VEGFs). The sequence of this protein, named GtVF, was inferred from the one base-substituted two cDNAs (438 bp) obtained via the 3′ RACE. The phylogenetic analysis suggested the presence of a new type of snake venom VEGFs including GtVF with no affinity to heparin in addition to the known three types of snake venom VEGFs with high affinity to heparin. Since the vascular permeability enhancement by GtVF was inhibited by the antibody against kinase insert domain-containing receptor (KDR), the vascular permeability enhancing activity of GtVF arises through KDR but without heparin binding.
- Published
- 2014
24. Asymmetric bioreduction of acetophenones by Baker's yeast and its cell-free extract encapsulated in sol–gel silica materials
- Author
-
Kazuma Nakanishi, Katsuya Kato, and Hitomi Nakamura
- Subjects
Silica gel ,General Physics and Astronomy ,Surfaces and Interfaces ,General Chemistry ,Condensed Matter Physics ,Surfaces, Coatings and Films ,Catalysis ,Thermogravimetry ,Solvent ,chemistry.chemical_compound ,chemistry ,Organic chemistry ,Methanol ,Fourier transform infrared spectroscopy ,Sol-gel ,Nuclear chemistry ,Acetophenone - Abstract
Baker's yeast (BY) encapsulated in silica materials was synthesized using a yeast cell suspension and its cell-free extract during a sol–gel reaction of tetramethoxysilane with nitric acid as a catalyst. The synthesized samples were fully characterized using various methods, such as scanning electron microscopy, nitrogen adsorption–desorption, Fourier transform infrared spectroscopy, thermogravimetry, and differential thermal analysis. The BY cells were easily encapsulated inside silica-gel networks, and the ratio of the cells in the silica gel was approximately 75 wt%, which indicated that a large volume of BY was trapped with a small amount of silica. The enzyme activity (asymmetric reduction of prochiral ketones) of BY and its cell-free extract encapsulated in silica gel was investigated in detail. The activities and enantioselectivities of free and encapsulated BY were similar to those of acetophenone and its fluorine derivatives, which indicated that the conformation structure of BY enzymes inside silica-gel networks did not change. In addition, the encapsulated BY exhibited considerably better solvent (methanol) stability and recyclability compared to free BY solution. We expect that the development of BY encapsulated in sol–gel silica materials will significantly impact the industrial-scale advancement of high-efficiency and low-cost biocatalysts for the synthesis of valuable chiral alcohols.
- Published
- 2014
25. Whole genome sequencing of a Japanese endemic pit viper, habu, Protobothrops flavoviridis reveals accelerated evolution of venom protein genes enriched in microchromosomal regions
- Author
-
Shosaku Hattori, Eiichi Shoguchi, Tomohisa Ogawa, Kazuaki Yamaguchi, Ryo Koyanagi, Noriyuki Satoh, Motonori Ohno, Kanako Hisata, Yoichi Matsuda, Hiroki Shibata, Hitomi Nakamura, Naoko Oda-Ueda, Yasuyuki Fukumaki, Kazumi Matsubara, Akiko Isomoto, and Takahito Chijiwa
- Subjects
Whole genome sequencing ,Genetics ,biology ,Pit viper ,Venom Protein ,Toxicology ,biology.organism_classification ,Gene ,Protobothrops flavoviridis - Published
- 2019
26. Analysis of the perinatal outcomes of advanced maternal age (AMA) model mice
- Author
-
Kiichiro Furuya, Hitomi Nakamura, Keiichi Kumasawa, Tsuyoshi Takiuchi, Tadashi Kimura, and Mika Handa
- Subjects
medicine.medical_specialty ,Reproductive Medicine ,business.industry ,Obstetrics ,Immunology ,Obstetrics and Gynecology ,Immunology and Allergy ,Medicine ,Advanced maternal age ,business - Published
- 2018
27. Genome-wide Association Study Identifies TNFSF15 and POU2AF1 as Susceptibility Loci for Primary Biliary Cirrhosis in the Japanese Population
- Author
-
Yoshihiko Maehara, Nobuyoshi Fukushima, Makoto Nakamuta, Kuniaki Arai, Noboru Hirashima, Hajime Takikawa, Kentaro Kikuchi, Yasuhiko Sugawara, Hiromasa Ohira, Shintaro Yagi, Yoshihiro Aiba, Akinobu Taketomi, Masakazu Kobayashi, Toshiki Komeda, Kazuhiro Sugi, Hirotoshi Ebinuma, Ken Shirabe, Jinya Ishida, Tatsuki Ichikawa, Minoru Nakamura, Katsuji Hirano, Hiromi Ishibashi, Kyoko Monoe, Eiichi Takesaki, Tatsuji Komatsu, Koichi Honda, Yasuni Nakanuma, Makiko Taniai, Masao Honda, Yasuhiro Miyake, Satoshi Yamagiwa, Kiyoshi Migita, Michiyasu Yagura, Toyokichi Muro, Yasuaki Takeyama, Kenichi Harada, Kazuhide Yamamoto, Atsushi Tanaka, Masanori Abe, Michiaki Koga, Yukio Watanabe, Masaaki Shimada, Morikazu Onji, Noriyo Yamashiki, Teru Kumagi, Masahiro Ito, Michio Yasunami, S. Tamura, Masataka Seike, Shuichi Kaneko, Takeji Umemura, Mikio Zeniya, Etsuko Hashimoto, Atsumasai Komori, Keisuke Ario, Naohiko Masaki, Nao Nishida, Norihiro Kokudo, Hiroki Takahashi, Kaname Yoshizawa, Hiroshi Yatsuhashi, Akira Mori, Takafumi Ichida, Kazuhiko Nakao, Hirohito Tsubouchi, Masashi Mizokami, Katsushi Tokunaga, Shinji Uemoto, Yoko Nakamura, Minae Kawashima, Hitomi Nakamura, Takeo Saoshiro, Keiichi Hirata, Satoru Joshita, Shinji Shimoda, Shotaro Sakisaka, Hirotaka Kouno, Hirotsugu Kouno, Hajime Ota, and Yoshiyuki Ueno
- Subjects
Adult ,Male ,Tumor Necrosis Factor Ligand Superfamily Member 15 ,Genome-wide association study ,Human leukocyte antigen ,Biology ,White People ,Young Adult ,Primary biliary cirrhosis ,Asian People ,HLA Antigens ,Report ,Genetics ,Genetic predisposition ,medicine ,Humans ,Genetic Predisposition to Disease ,Genetics(clinical) ,STAT4 ,Genetics (clinical) ,Aged ,Aged, 80 and over ,B-Lymphocytes ,Polymorphism, Genetic ,Liver Cirrhosis, Biliary ,Tumor Necrosis Factor-alpha ,Case-control study ,NF-kappa B p50 Subunit ,Cell Differentiation ,Odds ratio ,Middle Aged ,STAT4 Transcription Factor ,medicine.disease ,IKZF3 ,Genetic Loci ,Case-Control Studies ,Immunology ,Trans-Activators ,Female ,Genome-Wide Association Study - Abstract
For the identification of susceptibility loci for primary biliary cirrhosis (PBC), a genome-wide association study (GWAS) was performed in 963 Japanese individuals (487 PBC cases and 476 healthy controls) and in a subsequent replication study that included 1,402 other Japanese individuals (787 cases and 615 controls). In addition to the most significant susceptibility region, human leukocyte antigen (HLA), we identified two significant susceptibility loci, TNFSF15 (rs4979462) and POU2AF1 (rs4938534) (combined odds ratio [OR] = 1.56, p = 2.84 × 10(-14) for rs4979462, and combined OR = 1.39, p = 2.38 × 10(-8) for rs4938534). Among 21 non-HLA susceptibility loci for PBC identified in GWASs of individuals of European descent, three loci (IL7R, IKZF3, and CD80) showed significant associations (combined p = 3.66 × 10(-8), 3.66 × 10(-9), and 3.04 × 10(-9), respectively) and STAT4 and NFKB1 loci showed suggestive association with PBC (combined p = 1.11 × 10(-6) and 1.42 × 10(-7), respectively) in the Japanese population. These observations indicated the existence of ethnic differences in genetic susceptibility loci to PBC and the importance of TNF signaling and B cell differentiation for the development of PBC in individuals of European descent and Japanese individuals.
- Published
- 2012
28. Pulmonary nocardiosis caused by Nocardia exalbida complicating Pneumocystis pneumonia in an HIV-infected patient
- Author
-
Kentaro Imai, Tomohiko Koibuchi, Aikichi Iwamoto, Takeshi Fujii, Tadashi Kikuchi, Michiko Koga, Hitomi Nakamura, Toshiyuki Miura, Tohru Gonoi, and Katsukiyo Yazawa
- Subjects
Male ,Microbiology (medical) ,medicine.medical_specialty ,Nocardia Infections ,Pneumocystis pneumonia ,Nocardia ,Sputum culture ,Anti-Infective Agents ,Internal medicine ,Trimethoprim, Sulfamethoxazole Drug Combination ,Pneumonia, Bacterial ,medicine ,Humans ,Pharmacology (medical) ,Pentamidine ,AIDS-Related Opportunistic Infections ,medicine.diagnostic_test ,business.industry ,Pneumonia, Pneumocystis ,Nocardiosis ,Sputum ,Middle Aged ,medicine.disease ,Surgery ,Pneumonia ,Infectious Diseases ,Thienamycins ,Klebsiella pneumonia ,medicine.symptom ,Chest radiograph ,business ,Fluoroquinolones ,medicine.drug - Abstract
A 47-year-old man with optimally controlled type-2 diabetes mellitus and chronic hepatitis B was admitted to a local hospital because of a 1-week history of cough and high-grade fever. He was diagnosed with Pneumocystis pneumonia (PCP) and Klebsiella pneumonia from a chest radiograph and sputum. Simultaneously, he was found to have HIV infection with a CD4 count of 76/μl. Despite alteration of treatment secondary to the development of allergic reaction to trimethoprim-sulfamethoxazole (TMP-SMX), the patient was able to complete a 3-week therapy for PCP after being switched to pentamidine isetionate. After the treatment of PCP, he was referred to our hospital for the initiation of anti-HIV therapy. He presented with recurrent high-grade fever of a few days' duration prior to his initial visit, which subsequently led to his admission. Chest computed tomography (CT) showed the enlargement of a previously identified infiltrate in the left upper lung field, and the sputum culture upon admission was positive for Gram-positive branching rods; the organism was later identified as Nocardia exalbida. Due to his allergy to sulfonamide, the patient was treated with imipenem (IMP) and amikacin (AMK) given intravenously for 17 days, followed by garenoxacin (GRNX) taken orally for 6 months, without any adverse effects. The chest infiltrate resolved completely, and he remains stable without relapse 8 months after the completion of the therapy. Pulmonary nocardiosis should be considered as a differential diagnosis of recurring pneumonia in immunocompromised patients, especially in HIV-infected individuals. Oral administration of GRNX following IMP and AMK can be used as an alternative to TMP-SMX therapy in cases of pulmonary nocardiosis caused by N. exalbida.
- Published
- 2011
29. Long-term successful control of super-multidrug-resistant human immunodeficiency virus type 1 infection by a novel combination therapy of raltegravir, etravirine, and boosted-darunavir
- Author
-
Noriaki Hosoya, Michiko Koga, Takashi Odawara, Ai Kawana-Tachikawa, Toshiyuki Miura, Tadashi Kikuchi, Aikichi Iwamoto, Tomohiko Koibuchi, Takeshi Fujii, Naoko Miyazaki, and Hitomi Nakamura
- Subjects
Adult ,Male ,Microbiology (medical) ,Combination therapy ,Anti-HIV Agents ,Salvage therapy ,Etravirine ,HIV Infections ,Drug resistance ,Virus ,Drug Resistance, Multiple, Viral ,Raltegravir Potassium ,Nitriles ,medicine ,Humans ,Pharmacology (medical) ,Darunavir ,Salvage Therapy ,Sulfonamides ,business.industry ,Viral Load ,Raltegravir ,Virology ,Pyrrolidinones ,CD4 Lymphocyte Count ,Pyridazines ,Multiple drug resistance ,Pyrimidines ,Infectious Diseases ,HIV-1 ,RNA, Viral ,business ,medicine.drug - Abstract
Drug-resistant virus infection has been a major hurdle in the management of human immunodeficiency virus type 1 (HIV-1) infection. Recently, three novel antiretrovirals [raltegravir (RAL), etravirine (ETR), and darunavir (DRV)] were introduced into the market almost simultaneously, and salvage regimens containing these three antiretrovirals have been reported to exhibit strong potency against drug-resistant HIV-1 infection. However, the sustainability of such regimens remains unclear, particularly for patients infected with multidrug-resistant viruses. Here we report a case of super-multidrug-resistant HIV-1 infection which has been successfully controlled by novel combination therapy including RAL, ETR, and DRV for over 2 years, indicating that the novel combination could become an ultimate weapon against drug-resistant HIV infection and could alter the landscape of HIV salvage therapy.
- Published
- 2011
30. The dynamics of big mantle wedge, magma factory, and metamorphic–metasomatic factory in subduction zones
- Author
-
Akira Hasegawa, Kenji Kawai, Hitomi Nakamura, M. Santosh, Dapeng Zhao, Shigenori Maruyama, Tetsu Kogiso, and Soichi Omori
- Subjects
Underplating ,geography ,geography.geographical_feature_category ,Subduction ,Mantle wedge ,Volcanic arc ,Pacific Plate ,Geology ,Geophysics ,Plate tectonics ,Back-arc basin ,Convergent boundary ,Petrology - Abstract
The East Asian continental margin is underlain by stagnant slabs resulting from subduction of the Pacific plate from the east and the Philippine Sea plate from the south. We classify the upper mantle in this region into three major domains: (a) metasomatic–metamorphic factory (MMF), subduction zone magma factory (SZMF), and the ‘big mantle wedge’ (BMW). Whereas the convection pattern is anticlockwise in the MMF domain, it is predominantly clockwise in the SZMF and BMW, along a cross section from the south. Here we define the MMF as a small wedge corner which is driven by the subducting Pacific plate and dominated by H 2 O-rich fluids derived by dehydration reactions, and enriched in large ion lithophile elements (LILE) which cause the metasomatism. The SZMF is a zone intermediate between MMF and BMW domains and constitutes the main region of continental crust production by partial melting through wedge counter-corner flow. Large hydrous plume generated at about 200 km depth causes extensive reduction in viscosity and the smaller scale hydrous plumes between 60 km and 200 km also bring about an overall reduction in the viscosity of SZMF. More fertile and high temperature peridotites are supplied from the entrance to this domain. The domain extends obliquely to the volcanic front and then swings back to the deep mantle together with the subducting slab. The BMW occupies the major portion of upper mantle in the western Pacific and convects largely with a clockwise sense removing the eastern trench oceanward. Sporadic formation of hydrous plume at the depth of around 410 km and the curtain flow adjacent to the trench cause back arc spreading. We envisage that the heat source in BMW could be the accumulated TTG (tonalite–trondhjemite–granodiorite) crust on the bottom of the mantle transition zone. The ongoing process of transportation of granitic crust into the mantle transition zone is evident from the deep subduction of five intra-oceanic arcs on the subducting Philippine Sea plate from the south, in addition to the sediment trapped subduction by the Pacific plate and Philippine Sea plate. The dynamics of MMF, SZMF and BMW domains are controlled by the angle of subduction; a wide zone of MMF in SW Japan is caused by shallow angle subduction of the Philippine Sea plate and the markedly small MMF domain in the Mariana trench is due to the high angle subduction of Pacific plate. The domains in NE Japan and Kyushu region are intermediate between these two. During the Tertiary, a series of marginal basins were formed because of the nearly 2000 km northward shift of the subduction zone along the southern margin of Tethyan Asia, which may be related to the collision of India with Asia and the indentation. The volume of upper mantle under Asia was reduced extensively on the southern margin with a resultant oceanward trench retreat along the eastern margin of Asia, leading to the formation of a series of marginal basins. The western Pacific domain in general is characterized by double-sided subduction; from the east by the oldest Pacific plate and from the south by the oldest Indo-Australian plate. The old plates are hence hydrated extensively even in their central domains and therefore of low temperature. The cracks have allowed the transport of water into the deeper portions of the slab and these domains supply hydrous fluids even to the bottom of the upper mantle. Thus, a fluid dominated upper mantle in the western Pacific drives a number of microplates and promote the plate boundary processes.
- Published
- 2009
31. Contribution of slab-fluid in arc magmas beneath the Japan arcs
- Author
-
Hitomi Nakamura and Hikaru Iwamori
- Subjects
geography ,geography.geographical_feature_category ,Mantle wedge ,Subduction ,Volcanic arc ,Geology ,Geophysics ,Mantle (geology) ,Arc (geometry) ,Tectonics ,Slab ,Spatial variability ,Petrology - Abstract
Identifying the amount and composition of slab-derived fluid and its spatial variation is key to quantifying fluid processes in subduction zones. Based on the isotopic systematics of arc lavas, we found regional variations over the Japan arcs in terms of the amount and composition of slab-derived fluid added to the melting source region. The average amounts of slab-derived fluid differ among the arcs: 2.6 wt.% for Central Japan, 0.69 wt.% for Ryukyu, 0.17 wt.% for NE Japan, and 0.12 wt.% for both Kurile and Izu–Bonin. These differences may be attributed to the arc setting (oceanic or continental) and the geometry of the slabs. Contribution of sediment involved in the slab-derived fluid is dominant in NE Japan compared to the Izu–Bonin and Central Japan arcs. This could be attributed to mechanical features such as fractures near the subducting plate surface, in addition to the arc setting and the slab geometry. Therefore, the amount and composition of slab-derived fluid are thought to be controlled not only by the thermal conditions, but also by the tectonic and mechanical features around the subduction zone. On top of the variability of slab-derived fluid, the mantle wedge shows the regional variation in terms of proportion of the Pacific-type and Indian-type MORB-source mantle components, which also contributes to the compositional variations of arc magmas.
- Published
- 2009
32. Detection of serum and intrahepatic KL-6 in anti-HCV positive patients with hepatocellular carcinoma
- Author
-
Toshihiro Shimizu, Hitomi Nakamura, Hiroshi Matsumura, Atsuo Shioda, Naohide Tanaka, Yasuyuki Arakawa, Miki Kaneko, Hiroaki Yamagami, Azuma Watanabe, Mitsuhiko Moriyama, Hiroshi Aoki, Y. Arakawa, and Shuh Oshiro
- Subjects
Pathology ,medicine.medical_specialty ,Cirrhosis ,Hepatology ,Hepatitis C virus ,Biology ,medicine.disease ,medicine.disease_cause ,digestive system diseases ,Serology ,Infectious Diseases ,Antigen ,Hepatocellular carcinoma ,Cancer cell ,medicine ,Immunohistochemistry ,neoplasms ,MUC1 - Abstract
We investigated the clinical significance of serum and intrahepatic KL-6/MUC1 (KL-6) in patients with hepatitis C virus (HCV) antibody-positive hepatocellular carcinoma (HCC). The subjects included 76 patients diagnosed with anti-HCV positive HCC, 69 with, and 51 without, liver cirrhosis (LC). Frozen serum samples were obtained from each subject to determine the serum KL-6 levels using an enzyme-linked immunosorbent assay. Expression of KL-6 antigen in the liver was also investigated using immunoperoxidase staining. The mean serum KL-6 level in patients with HCC was [Formula: see text] U/ml (319U/ml for HCC with LC, 342.8U/ml for HCC without LC). Serum KL-6 levels in patients with HCC with LC and HCC without LC did not differ. Serum KL-6 levels were elevated with increases in the size of spaces occupied by tumors in the liver. Among patients with HCC, there was no correlation between serum KL-6 levels and alpha-fetoprotein (AFP) levels and protein induced by vitamin K absence or antagonist-II (PIVKA-II) levels. However, some patients with low levels of AFP and PIVKA-II possessed high levels of KL-6. Furthermore, serum KL-6 levels decreased after therapy for HCC nodules. Immunohistochemical staining showed KL-6 antigen was detected within the cell membrane and in the cytoplasm of cancer cells. KL-6 antigen was localized on the membrane and the endoplasmic reticulum of cancer cells in the cancerous foci by electron microscopy. Our results suggest that serum KL-6 levels represent a serological marker of HCC development, because KL-6 expression was localized to the cancer cells in HCC nodules.
- Published
- 2004
33. Detection of oxidative stress in seminal plasma and fractionated sperm from subfertile male patients
- Author
-
Tadashi Kimura, Shigeyuki Isaka, Kazumasa Hashimoto, Hitomi Nakamura, Atsuko Nakajima, Masayasu Koyama, Yuji Murata, Chihiro Azuma, Masahiko Takemura, and Koichiro Shimoya
- Subjects
Adult ,Male ,Lipid Peroxides ,endocrine system ,medicine.medical_specialty ,Thiobarbituric acid ,Enzyme-Linked Immunosorbent Assay ,Semen ,Biology ,medicine.disease_cause ,Asthenozoospermia ,Thiobarbituric Acid Reactive Substances ,chemistry.chemical_compound ,Malondialdehyde ,Internal medicine ,Centrifugation, Density Gradient ,medicine ,Humans ,Reproductive system ,Infertility, Male ,Lipid peroxide ,urogenital system ,Deoxyguanosine ,Obstetrics and Gynecology ,medicine.disease ,Spermatozoa ,Sperm ,Oxidative Stress ,Endocrinology ,Reproductive Medicine ,chemistry ,8-Hydroxy-2'-Deoxyguanosine ,Percoll ,Oxidative stress - Abstract
Objectives: Oxidative stress in the reproductive system is thought to affect the fertilizing ability of sperm. Since 8-hydroxydeoxyguanosine (8-OHdG) and lipid peroxides are widely used as markers to quantify oxidative stress, we compared 8-OHdG and lipid peroxide concentrations in seminal plasma and spermatozoa from subfertile and fertile men. Study design: Semen obtained from 37 men of subfertile couples (21 men with normozoospermia and 16 with asthenozoospermia) and from eight fertile volunteers were examined. Seminal plasma and spermatozoa were fractionated by four-step discontinuous Percoll gradient centrifugation. 8-OHdG in seminal plasma was measured by ELISA, and lipid peroxides in seminal plasma and spermatozoa were determined using a thiobarbituric acid (TBA) assay. Results: The concentrations of 8-OHdG and lipid peroxides in the seminal plasma of the subfertile group were significantly higher than those of the fertile group. There were no significant differences in these values between patients with normozoospermia and asthenozoospermia. In all four fractions obtained by Percoll gradient fractionation, the lipid peroxide levels in spermatozoa recovered from subfertile males were significantly higher than those of fertile controls. Conclusions: Seminal plasma and spermatozoa from subfertile males showed elevated levels of oxidative stress that were detectable in ejaculated semen specimens by ELISA or TBA assay. Even the spermatozoa fraction considered to be mature and normal showed elevated oxidative stress in the subfertile group. Our results confirm the importance of oxidative stress in male reproductive function, and could be applied for the selection of patients for antioxidant therapy.
- Published
- 2002
34. Hemagglutinating Virus of Japan (HVJ) Envelope Vector as a Versatile Gene Delivery System
- Author
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Hiroyuki Ikegami, Yasufumi Kaneda, Hitoshi Kotani, Hitomi Nakamura, Tomoyuki Nishikawa, Ryuichi Morishita, Naho Suzuki, Toshihiro Nakajima, and Seiji Yamamoto
- Subjects
Genetic enhancement ,Genetic Vectors ,Gene delivery ,Sendai virus ,Virus ,Cell Line ,Mice ,Viral envelope ,Genes, Reporter ,Drug Discovery ,Genetics ,Animals ,Humans ,Luciferases ,Molecular Biology ,Pharmacology ,Reporter gene ,biology ,Genetic Therapy ,biology.organism_classification ,Molecular biology ,Oligodeoxyribonucleotides ,Cell culture ,Molecular Medicine ,Ex vivo ,Fluorescein-5-isothiocyanate - Abstract
We have developed a simple method for converting the lipid envelope of an inactivated virus to a gene transfer vector. Hemagglutinating virus of Japan (HVJ; Sendai virus) envelope vector was constructed by incorporating plasmid DNA into inactivated HVJ particles. This HVJ envelope vector introduced plasmid DNA efficiently and rapidly into various cell lines, including cancer cells and several types of primary cell culture. Efficiency of gene transfer was greatly enhanced by protamine sulfate and centrifugation. Fluorescein isothiocyanate-labeled oligodeoxynucleotides (FITC-ODN) were also delivered to cells at > 95% efficiency. When HVJ envelope vector was injected into organs directly, reporter gene expression was observed in organs including liver, brain, skin, uterus, tumor masses, lung, and eye. When HVJ envelope vector containing luciferase gene was injected into mouse tail vein, luciferase gene expression was detected primarily in spleen. FITC-ODN were also delivered to spleen cells by intravenous injection of HVJ envelope. These results suggest that HVJ envelope vector will be useful for both ex vivo and in vivo gene therapy experiments.
- Published
- 2002
- Full Text
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35. DNA Methylation of the Human Oxytocin Receptor Gene Promoter Regulates Tissue-Specific Gene Suppression
- Author
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Masayasu Koyama, Chihiro Azuma, Kazuhide Ogita, Tadashi Kimura, Yoko Matsumura, Chika Kusui, Yuji Murata, and Hitomi Nakamura
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Biophysics ,Biology ,Transfection ,Biochemistry ,Genes, Reporter ,Transcription (biology) ,Tumor Cells, Cultured ,Humans ,Gene Silencing ,RNA, Messenger ,Promoter Regions, Genetic ,Molecular Biology ,Reporter gene ,Expression vector ,Tissue-Specific Gene Expression ,Promoter ,Cell Biology ,Methylation ,DNA Methylation ,Molecular biology ,Liver ,CpG site ,Organ Specificity ,Receptors, Oxytocin ,DNA methylation ,Azacitidine ,CpG Islands - Abstract
In the human oxytocin receptor (OTR) gene, there is a CpG island from 140 bp upstream to 2338 bp downstream of the transcription start site (TSS). We investigated whether the methylation state of this region affects the transcription of the OTR gene. HepG2 derived from human hepatoblastoma, in which OTR gene transcription was suppressed, was treated with a demethylating agent, 5-azacytidine (Aza-C) for 2 days. Semiquantitative RT-PCR indicated that OTR mRNA was significantly increased by Aza-C treatment in a dose-dependent manner. We estimated the level of methylation within the CpG islands of the OTR gene in peripheral blood leukocytes, nonpregnant uterine myometrium, term uterine myometrium and liver. A 1.5-kb region located 5' upstream of the translation start site was divided into four fragments. Each was amplified by PCR after complete digestion with methylation-sensitive restriction enzyme HpaII. The amount of PCR products was largest in the liver, suggesting that this CpG island in the OTR gene is most highly methylated in liver, where the gene is always inactivated. We compared the effect of in vivo methylation of the CpG island on transcriptional activity of an OTR-reporter plasmid. The reporter gene activity of expression plasmid -2860/+1342-GL3, containing the CpG island, in HepG2 cells was suppressed to 30.6% of the control level after methylation with SssI methylase, while that of -2840/+144-GL3, without the CpG island was suppressed only to 81.4%. The deletion of the segment (MT2) where the level of methylation was most different between liver and uterus (-2860/+1342(del)MT2-GL3) rescued the suppression rate to 68.0%. These results indicate that the methylation of the CpG island in the human OTR gene promoter suppressed its transcription at least in liver and may regulate tissue specific gene expression among organs.
- Published
- 2001
36. A case report of a successful pregnancy in a woman diagnosed with common variable immunodeficiency with impaired B cell class-switching and the decrease in naïve CD4 positive T cell
- Author
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Yosuke Suzuki, Keiichi Kumasawa, Noriko Sato, Tadashi Kimura, Kaori Koizumi, Satoko Goa, Naoya Shigeta, Michiko Yamashita, Yuri Yasui, Ayaka Tanaka, Kiichiro Furuya, Hitomi Nakamura, Namhyo Kim, and Yayoi Fukuda
- Subjects
business.industry ,Common variable immunodeficiency ,T cell ,Immunology ,Obstetrics and Gynecology ,Successful pregnancy ,medicine.disease ,medicine.anatomical_structure ,Reproductive Medicine ,Immunoglobulin class switching ,medicine ,Immunology and Allergy ,business ,B cell - Published
- 2016
37. Soluble VEGF receptor 1(sFLT1) induces non-apoptotic cell death of ovarian and colorectal cancer cells
- Author
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Tadashi Kimura, Noriko Sato, Keiichi Kumasawa, Tsuyoshi Takiuchi, Hitomi Nakamura, and Tatsuya Miyake
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Oncology ,medicine.medical_specialty ,biology ,business.industry ,Colorectal cancer ,VEGF receptors ,Obstetrics and Gynecology ,medicine.disease ,Reproductive Medicine ,Internal medicine ,Apoptotic cell death ,biology.protein ,Medicine ,business ,Developmental Biology - Published
- 2016
38. Reply to comment on 'Origin of methane in serpentinite-hosted hydrothermal systems: The CH4–H2–H2O hydrogen isotope systematics of the Hakuba Happo hot spring' by Suda et al. [Earth Planet. Sci. Lett. 386 (2014) 112–125]
- Author
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Keita Yamada, Yuichi Hongoh, Yuichiro Ueno, Ken Kurokawa, Koji Yoshino, Eri Nishiyama, Kenichi Kawachi, Naohiro Yoshida, Shigenori Maruyama, Konomi Suda, Soichi Omori, Motoko Yoshizaki, and Hitomi Nakamura
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Systematics ,Hot spring ,Earth science ,Hydrogen isotope ,Hydrothermal circulation ,Methane ,Astrobiology ,chemistry.chemical_compound ,Geophysics ,chemistry ,Space and Planetary Science ,Geochemistry and Petrology ,Planet ,Earth and Planetary Sciences (miscellaneous) ,Geology ,Earth (classical element) - Published
- 2014
39. 195. A Novel Vascular Endothelial Growth Factor-Like Protein from Gloydius tsushimaensis Venom
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Michihisa Toriba, Takahisa Imamura, Motonori Ohno, Naoko Oda-Ueda, Tatsuo Murakami, Takahito Chijiwa, and Hitomi Nakamura
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Vascular endothelial growth factor ,chemistry.chemical_compound ,Gloydius tsushimaensis ,chemistry ,biology ,Venom ,Pharmacology ,Toxicology ,biology.organism_classification - Published
- 2012
40. Effective encapsulation of biocatalysts in sol–gel silica nanosheet prepared by peptide catalysts
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Hitomi Nakamura, Fukue Nagata, and Katsuya Kato
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chemistry.chemical_classification ,Chemical engineering ,Chemistry ,Organic chemistry ,Bioengineering ,Peptide ,General Medicine ,Molecular Biology ,Biotechnology ,Catalysis ,Nanosheet ,Sol-gel ,Encapsulation (networking) - Published
- 2014
41. Superconductivity in Y2PdGe3−Si : interplay between Debye temperature and coupling constant
- Author
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Masashi Tokunaga, Tsuyoshi Tamegai, Ajay Ghosh, and Hitomi Nakamura
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Coupling constant ,Superconductivity ,Materials science ,Condensed matter physics ,Transition temperature ,Intermetallic ,Energy Engineering and Power Technology ,Condensed Matter Physics ,Debye frequency ,Electronic, Optical and Magnetic Materials ,Magnetization ,symbols.namesake ,Electrical resistivity and conductivity ,symbols ,Electrical and Electronic Engineering ,Debye model - Abstract
We have studied Y 2 PdGe 3− x Si x superconductor using magnetization and specific heat measurements. The compounds are very sensitive to changes in the honeycomb layers by Si substitution. The critical temperature shows a maximum of T c =3.55 K near x =0.3 and decreases with further increase in Si concentration. The changes in the Debye frequency and electron–phonon coupling have combined effect to control the critical temperature.
- Published
- 2003
42. 1065 Sen virus infection influence the pathological findings in liver, but not affect the incidence of carcinoma
- Author
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Yasuyuki Arakawa, Atsuo Shioda, Shyu Oshiro, Mitsuhiko Moriyama, Hiroshi Matsumura, Miki Kaneko, Hiroshi Aoki, and Hitomi Nakamura
- Subjects
Pathology ,medicine.medical_specialty ,Hepatology ,business.industry ,Incidence (epidemiology) ,Immunology ,Carcinoma ,Medicine ,Sen virus ,business ,medicine.disease ,Affect (psychology) ,Pathological - Published
- 2003
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