6 results on '"Helen Sadauskas-Henrique"'
Search Results
2. Response of Oreochromis niloticus (Teleostei: Cichlidae) exposed to a guanitoxin-producing cyanobacterial strain using multiple biomarkers
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Larissa Souza, Passos, Levy Carvalho, Gomes, Tatiana Miura, Pereira, Helen, Sadauskas-Henrique, Giorgi Dal, Pont, Antonio, Ostrensky, and Ernani, Pinto
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Environmental Engineering ,Tandem Mass Spectrometry ,Acetylcholinesterase ,Animals ,Environmental Chemistry ,Cichlids ,Cyanobacteria ,Pollution ,Waste Management and Disposal ,Biomarkers ,Ecosystem ,Chromatography, Liquid ,Mutagens - Abstract
Changes in environmental conditions in aquatic ecosystems caused by anthropic actions can modify the composition of primary producers, promoting the excessive proliferation of cyanobacteria. These organisms can form cyanobacterial blooms, which directly affect aquatic life. The present study investigated the mutagenicity of the cyanobacterium Sphaerospermopsis torques-reginae (strain ITEP-024), guanitoxin-producing (natural organophosphate), and sublethal effects on fish in relevant environment concentrations. For this, the Ames test (Salmonella/microsome) was performed as a mutagenic assay for extracts of the ITEP-024 strain. Specimens of Oreochromis niloticus (Teleostei: Cichlidae) were subjected to acute 96 h exposure to different concentrations of aqueous extract of the strain: C = control group; T1 = 31.25 mg/L; T2 = 62.5 mg/L; T3 = 125 mg/L; and T4 = 250 mg/L. Genotoxic, biochemical, osmoregulatory, and physiologic biomarkers were analyzed. Our results showed that the cyanobacterium had a weak mutagenic response for the TA102 strain of Salmonella with and without metabolic activation by S9. Strains TA98 and TA100 were not affected. Fish from treatments T3 and T4 showed changes in oxidative stress (CAT, SOD, and GST enzymes), inhibition of the enzyme acetylcholinesterase activity, micronucleus formation, and osmoregulatory disorders. No guanitoxin accumulation was detected in the different tissues of O. niloticus by LC-MS/MS. Our results showed unprecedented mutagenicity data of the guanitoxin-producing cyanobacteria by the Ames test and biochemical, osmoregulatory, and genotoxic disorders in fish, providing efficient aquatic contamination biomarkers. Despite the great concern related to the presence of guanitoxin in blooms in freshwater ecosystems, its concentration is not yet regulated, and thus there is no monitoring agenda in current legislation.
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- 2022
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3. Validation of a suite of biomarkers of fish health in the tropical bioindicator species, tambaqui ( Colossoma macropomum )
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Marthe Monique Gagnon, Rafael M. Duarte, Vera Maria Fonseca de Almeida-Val, Helen Sadauskas-Henrique, Laboratory of Ecophysiology and Molecular Evolution, Universidade Estadual Paulista (Unesp), and Curtin University
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0301 basic medicine ,Antioxidant ,DNA damage ,Tambaqui ,medicine.medical_treatment ,LPO ,Intraperitoneal injection ,General Decision Sciences ,010501 environmental sciences ,BaP ,s-SDH ,01 natural sciences ,Toxicology ,Andrology ,Superoxide dismutase ,Lipid peroxidation ,03 medical and health sciences ,chemistry.chemical_compound ,medicine ,GST ,SOD ,Ecology, Evolution, Behavior and Systematics ,0105 earth and related environmental sciences ,chemistry.chemical_classification ,EROD activity ,Ecology ,biology ,CAT ,biology.organism_classification ,030104 developmental biology ,Enzyme ,chemistry ,Catalase ,biology.protein ,Biomarkers - Abstract
Made available in DSpace on 2018-12-11T17:07:35Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-02-01 Financiadora de Estudos e Projetos Here we explore the dose-dependent response of the tropical fish tambaqui (Colossoma macropomum) to intraperitoneal injection of benzo[a]pyrene (BaP) at doses of 0 (carrier control), 1, 10, 100 and 1000 μmolar BaP Kg−1 Hepatic ethoxyresorufin-O-deethylase (EROD) activity showed a bell-shaped dose-dependent response curve, where the highest injected BaP dose caused enzyme inactivation. Activities of hepatic catalase (CAT) and superoxide dismutase (SOD) increased at the highest dose relative to the carrier control group. Lipid peroxidation (LPO), serum-sorbitol dehydrogenase (s-SDH) and DNA damage in blood cells were higher for all BaP doses when compared to the carrier control group. At high dosage, the production of BaP metabolites was paralleled by induced activity of the antioxidant enzyme SOD, and high levels of DNA damage in blood cells. In a similar way, high LPO was concomitant to elevated s-SDH in the bloodstream, suggesting that lipid peroxidation caused the loss of membrane integrity and leakage of s-SDH from hepatocytes into the bloodstream. These biomarkers were also positively co-correlated. The results demonstrate the potential use of a suite of biomarkers for tambaqui living in contaminated tropical aquatic environments. In particular, we recommend the analysis of DNA damage in blood cells, as this was highly correlated with all other biomarkers. Brazilian National Institute for Research in the Amazon Laboratory of Ecophysiology and Molecular Evolution, Ave André Araújo 2936 Biosciences Institute São Paulo State University − UNESP Coastal Campus, Pça Infante Dom Henrique s/n°, P.O. Box 73601, São Vicente Department of Environment and Agriculture Curtin University, P.O. Box U1987 Biosciences Institute São Paulo State University − UNESP Coastal Campus, Pça Infante Dom Henrique s/n°, P.O. Box 73601, São Vicente
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- 2017
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4. Biometric and hematological indices of Mugil curema inhabiting two Neotropical estuaries
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Matheus Marcos Rotundo, Camilo Dias Seabra Pereira, Ursulla Pereira Souza, Helen Sadauskas-Henrique, and Laís Henrique Cicero
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0106 biological sciences ,geography ,geography.geographical_feature_category ,010504 meteorology & atmospheric sciences ,Ecology ,Mugil ,010604 marine biology & hydrobiology ,Zoology ,Estuary ,Aquatic Science ,Biology ,Seasonality ,medicine.disease ,biology.organism_classification ,01 natural sciences ,Salinity ,medicine ,%22">Fish ,Animal Science and Zoology ,Ecology, Evolution, Behavior and Systematics ,0105 earth and related environmental sciences - Abstract
The present study aimed to evaluate the biometric and hematological indices in Mugil curema collected in two Neotropical estuaries in the south coast of Brazil, and to investigate if such variables can be influenced by seasonality and degree of anthropogenic influence. Differences between seasons and estuarine systems were observed, where the dissolved oxygen and salinity, together with the historical contamination of the Santos estuarine system (SES), seem to play an important role in the biological response. Fish from the SES presented, in general, high responses of the hematological variables (red and white series) and the hepatosomatic index, relative to those fish from Peruibe estuarine system (PES), even with the remote contamination present at PES, while no erythrocytic abnormalities or micronucleus were observed in fish collected at SES and PES in both seasons. The present work demonstrated that M. curema collected at SES are experiencing hematological and metabolic perturbations that are not observed in fish collected at PES.
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- 2020
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5. The impact of organochlorines and metals on wild fish living in a tropical hydroelectric reservoir: bioaccumulation and histopathological biomarkers
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M. N. Fernandes, Marise Margareth Sakuragui, Helen Sadauskas-Henrique, Tayrine Paschoaletti Benze, João B. Fernandes, and Marcelo Gustavo Paulino
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Gill ,Veterinary medicine ,animal structures ,Environmental Engineering ,Heptachlor Epoxide ,Heptachlor ,Biology ,chemistry.chemical_compound ,Dieldrin ,Hydrocarbons, Chlorinated ,Animals ,Environmental Chemistry ,Aldrin ,Waste Management and Disposal ,Endosulfan ,Ecology ,Fishes ,Contamination ,Pollution ,chemistry ,Metals ,Bioaccumulation ,Biomarkers ,Brazil ,Water Pollutants, Chemical ,Environmental Monitoring - Abstract
This study evaluates the contaminants in water and their bioaccumulation in the gills and liver of two ecologically distinct fish species, Astyanax fasciatus and Pimelodus maculatus, living in the reservoir of the Furnas hydroelectric power station located in Minas Gerais in the southeastern Brazil. The histological alterations in these organs are also examined. Water and fish were collected in June and December from five sites (site 1: FU10, site 2: FU20, site 3: FU30, site 4: FU40 and site 5: FU50) in the reservoir, and agrochemicals and metals selected based on their use in the field crops surrounding the reservoir were analyzed in the water and in the fish gills and livers. The concentrations of the organochlorines aldrin/dieldrin, endosulfan and heptachlor/heptachlor epoxide as well as the metals copper, chromium, iron and zinc in the gills and livers of both fish species were higher in June than in December; the liver accumulated higher concentrations of contaminants than the gills. The organochlorine metolachlor was detected only in the liver. The histological pattern of changes was similar in both species with regard to contaminant accumulation in the gills and liver. Fish from FU10, the least contaminated site, exhibited normal gill structure and moderate to heavy liver damage. Fish collected at FU20 to FU50, which were contaminated with organochlorines and metals, showed slight to moderate gill damage in June and irreparable liver damage in the livers in June and December. The histological changes in the gills and liver were suitable to distinguishing contaminated field sites and are therefore useful biomarkers for environmental contamination representing a biological end-point of exposure.
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- 2014
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6. Integrated use of antioxidant enzymes and oxidative damage in two fish species to assess pollution in man-made hydroelectric reservoirs
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Marisa Narciso Fernandes, Marcelo Gustavo Paulino, Cleoni dos Santos Carvalho, Marise Margareth Sakuragui, Camilo Dias Seabra Pereira, and Helen Sadauskas-Henrique
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Gills ,Antioxidant ,Health, Toxicology and Mutagenesis ,medicine.medical_treatment ,Toxicology ,medicine.disease_cause ,Lipid peroxidation ,Superoxide dismutase ,Dieldrin ,chemistry.chemical_compound ,Hydrocarbons, Chlorinated ,medicine ,Animals ,chemistry.chemical_classification ,Glutathione Peroxidase ,biology ,Superoxide Dismutase ,Glutathione peroxidase ,Fishes ,General Medicine ,Pollution ,Oxidative Stress ,Liver ,chemistry ,Catalase ,Bioaccumulation ,Environmental chemistry ,biology.protein ,Metallothionein ,Lipid Peroxidation ,Brazil ,Water Pollutants, Chemical ,Oxidative stress ,Environmental Monitoring ,Power Plants - Abstract
This study investigated the relationship between contaminant body burden and the oxidative stress status of the gills and livers of two wild fish species in the Furnas Hydroelectric Power Station (HPS) reservoir (Minas Gerais, Brazil). Gills and livers presented similar pathways of metals and organochlorine bioaccumulation. During June, organochlorines were associated with lipid peroxidation (LPO), indicating oxidative stress due to the inhibition of the antioxidant enzymes superoxide dismutase and glutathione peroxidase. In the most polluted areas, metal concentrations in the liver were associated with metallothionein. During December, contaminants in the gills and liver were associated with catalase activity and LPO. Aldrin/dieldrin was the contaminant most associated with oxidative damage in the livers of both species. This integrated approach shed light on the relationship between adverse biological effects and bioaccumulation of contaminants inputted by intensive agricultural practices and proved to be a suitable tool for assessing the environmental quality of man-made reservoirs.
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- 2013
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