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Your search keyword '"H.-H. Brackmann"' showing total 4 results
Start Over Author "H.-H. Brackmann" Publisher elsevier bv
4 results on '"H.-H. Brackmann"'

1. A G+3-to-T Donor Splice Site Mutation Leads to Skipping of Exon 50 in Von Willebrand Factor mRNA

Author

Günter Mertes, Michael Ludwig, Klaus Olek, Michael Krawczak, R Schwaab, B. Finkelnburg, and H H Brackmann

Subjects
Male, Guanine, RNA Splicing, Molecular Sequence Data, Biology, Gene mutation, medicine.disease_cause, law.invention, Exon, Von Willebrand factor, law, von Willebrand Factor, Genetics, medicine, Humans, RNA, Messenger, Cells, Cultured, Polymerase chain reaction, DNA Primers, Mutation, Base Sequence, Exons, Molecular biology, Exon skipping, genomic DNA, RNA splicing, biology.protein, Female, Thymine
Abstract

Genomic DNA was prepared from leukocytes, and platelet-derived total mRNA was isolated by lysis of thrombocytes in 4 M guanidinium hydrochloride followed by precipitation in 2 M lithium chloride twice. PCR reactions were performed using either RT-PCR primers or genomic PCR-primers. Preferential signle-strand amplification for both PCR products was carried out with nested RT-PCR primers.

Published
1994

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