21 results on '"Guanglin He"'
Search Results
2. Dissolved gas in transformer oil forecasting for transformer fault evaluation based on HATT-RLSTM
- Author
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Mingwei Zhong, Yunfei Cao, Guanglin He, Lutao Feng, Zhichao Tan, Wenjun Mo, and Jingmin Fan
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Energy Engineering and Power Technology ,Electrical and Electronic Engineering - Published
- 2023
3. Molecular genetic survey and forensic characterization of Chinese Mongolians via the 47 autosomal insertion/deletion marker
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Xing Zou, Shouyu Wang, Jing Liu, Shuang Gao, Zheng Wang, Fuquan Jia, Guanglin He, Yiping Hou, Ziwei Ye, and Mengge Wang
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0106 biological sciences ,China ,Population ,Biology ,01 natural sciences ,03 medical and health sciences ,Asian People ,Gene Frequency ,INDEL Mutation ,parasitic diseases ,Ethnicity ,otorhinolaryngologic diseases ,Genetics ,Humans ,Insertion deletion ,education ,Indel ,Molecular Biology ,030304 developmental biology ,0303 health sciences ,education.field_of_study ,Forensic science ,Genetics, Population ,Homogeneous ,Evolutionary biology ,Genetic relatedness ,Phylogenetic relationship ,010606 plant biology & botany - Abstract
The Mongolians are mainly distributed in the modern state of Mongolia, China, Russia, and other countries. While the historic and archaeological records of the rise and fall of the Mongol Empire are well documented, little has been known about the genetic legacy of modern Mongolian populations. Here, 611 Mongolian individuals from Hohhot, Hulunbuir, and Ordos of China were genotyped via the 47 Insertion/Deletion markers. Forensically statistical parameters indicated that this InDel system could be applied to forensic investigation in Mongolian populations. The comprehensive population comparisons indicated that targeted Mongolian populations are a homogeneous population, which kept close genetic proximity with geographically northern East Asians. The findings of the model-based clustering analysis revealed a southern East Asian-specific ancestral component, which was maximized in Hainan Li, and Mongolian populations harbored relatively less Hainan Li-related ancestry and more northern East Asian-related ancestry compared with reference Tai-Kadai, Austroasiatic and Sinitic people.
- Published
- 2021
4. Forensic features and genetic structure at insertion/deletion polymorphisms of Chinese Han with other 31 Chinese ethnic groups
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Hong Zhu, Yiping Hou, Guanglin He, Zheng Wang, and Xing Zou
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education.field_of_study ,Phylogenetic tree ,010401 analytical chemistry ,Population ,Ethnic group ,Population genetics ,Ethnic origin ,Biology ,01 natural sciences ,0104 chemical sciences ,Pathology and Forensic Medicine ,03 medical and health sciences ,0302 clinical medicine ,Evolutionary biology ,Genetic structure ,Genetics ,030216 legal & forensic medicine ,Indel ,education ,Allele frequency - Abstract
Insertion/deletion polymorphisms (InDels) have been used in population genetics, anthropology, archaeology and forensic. Chinese Han population with 1.3 billion people is the largest homogeneous ethnicity in the world. Here, we reanalyzed the allele frequency distributions and forensic parameters of 30 InDels included in Qiagen Investigator DIPplex kit in a Meta-Han Chinese population and then explored the genetic affinity between Meta-Han group and other 31 Chinese ethnic populations belonging to different administrative divisions using Fst genetic distances, multidimensional scaling (MDS), phylogenetic analysis and Structure. The values of CPD and CPE in Han Chinese are 0.999999999984 and 0.9879 respectively, which indicating this panel is informative in Han Chinese and can be used for forensic individual identification, while just be served as a supplementary tool for parentage testing. The comprehensive population comparisons reveal that Han Chinese have the genetically closer relationship with Fujian She and Zunyi Miao. Findings from pairwise genetic distances, MDS and phylogenetic analysis demonstrate that genetic affinity is related to ethnic origin, geographical distribution and linguistic background. STRUCTURE analysis indicates that the same language or ethnic origin populations or geographically adjacent groups have similar ancestry composition.
- Published
- 2019
5. Revisiting the matrilineal lineages and hypoxic adaptation of highland Tibetans
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Guanglin He, Mengge Wang, Yiping Hou, and Zheng Wang
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Nonsynonymous substitution ,Mitochondrial DNA ,Evolutionary biology ,Genetics ,Biology ,Adaptation ,Genome ,Gene ,Haplogroup ,Pathology and Forensic Medicine - Abstract
The mitochondrial DNA (mtDNA) plays a vital role in forensic, anthropological, biogeographical and genealogical studies. In the present study, we sequenced 59 mitochondrial genomes of Tibetan individuals settling in Muli Tibetan Autonomous County of Sichuan Province using the Precision ID mtDNA Whole Genome Panel and Ion S5 XL system. Meanwhile, 192 published complete mitogenomes from five Tibetan populations were included for further analysis. All 251 investigated Tibetan participants were assigned to 98 unique subclades pertained to the macrohaplogroups M and N, and 17 subhaplogroups were considered as major haplogroups of Tibetans since these subhaplogroups accounted for considerably high frequencies in randomly selected Tibetans. It was noteworthy that M9a1a1c1b1a was the predominant subhaplogroup in the Tibetans collectively. Furthermore, the nonsynonymous substitutions and synonymous substitutions ratios (N/S) of Tibetans, Tibetan highlanders (Monpas, Lhobas, Dengs and Sherpas), non-Tibetan highlanders and general populations were estimated to evaluate the potential selective constraints. The N/S ratio in the Tibetan groups (0.503) is higher than that in Tibetan highlanders (0.465), non-Tibetan highlanders (0.430) and general populations (0.415). The distributions of N/S ratio in 13 protein-coding genes revealed that significant differences were existed in COX2, ATP8 genes, which likely contributed to hypoxic adaptation.
- Published
- 2019
6. A comprehensive exploration of the genetic legacy and forensic features of Afghanistan and Pakistan Mongolian-descent Hazara
- Author
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Allah Rakha, Hui-Yuan Yeh, Abulhasan Fawad, Chuan-Chao Wang, Atif Adnan, Xing Zou, Guanglin He, Jianxin Guo, Muhammad Rehman, Pengyu Chen, and Mengge Wang
- Subjects
Genetic Markers ,Male ,0301 basic medicine ,Genotype ,Population ,Datasets as Topic ,Genetic admixture ,Kazakh ,Polymerase Chain Reaction ,Genetic analysis ,Pathology and Forensic Medicine ,03 medical and health sciences ,0302 clinical medicine ,Gene Frequency ,INDEL Mutation ,Ethnicity ,Genetics ,Humans ,Pakistan ,030216 legal & forensic medicine ,education ,Indel ,Allele frequency ,education.field_of_study ,Afghanistan ,Mongolia ,DNA Fingerprinting ,language.human_language ,Genetics, Population ,030104 developmental biology ,Geography ,Evolutionary biology ,Genetic structure ,language ,Female ,Microsatellite Repeats - Abstract
Afghanistan and Pakistan are rich with a complex landscape of culture, linguistics, ethnicity and genetic legacy at the crossroads between Indian-Subcontinent and Central Asia. Hazara people have historically been suggested to be Mongolian decedents but seldom been genetically studied. To dissect the genetic structure and explore the forensic characteristics of Hazara people, we first genotyped 30 Insertion/deletion (Indel) markers in 468 samples from 2 aboriginal Hazara populations from Afghanistan and Pakistan, and 100 East Asian comparative Bouyei samples using the Investigator® DIPplex kit. Subsequently, we carried out a comprehensive population genetic analysis from four different datasets: 8895 30-Indel genotype data from 51 populations, 15,895 30-Indel allele frequency data from 98 populations, 1048 genotypes of 993 STRs and Indels from 53 HGDP populations and 2068 whole-genomes (621,799 single nucleotide polymorphisms) from 165 worldwide Human origin reference populations, to further unravel the genetic complexity between Hazara and worldwide human populations using various statistical analysis. We find that 30 Indels are in accordance with HWE, and informative and polymorphic in both Central Asians Hazara and East Asian Bouyei populations. The forensic combined probability of exclusion is larger than 0.9943 and the cumulative power of discrimination is larger than 0.99999999999936. These forensic parameters show the high level of diversity, which makes the Indel amplification system suitable for forensic routine work and may be used as a supplementary assay for routine forensic investigation. The results from pairwise genetic distances, MDS, PCA, and phylogenetic relationship reconstruction demonstrate that present-day Hazaras are genetically closer to the Turkic-speaking populations (Uyghur, Kazakh, and Kyrgyz) residing in northwest China than with other Central/South Asian populations and Mongolian. Outgroup and admixture f3,f4, f4-ratio, qpWave, and qpAdm results further demonstrate that Hazara shares more alleles with East Asians than with other Central Asians and carries 57.8% Mongolian-related ancestry. Overall, our findings suggest that Hazaras have experienced genetic admixture with the local or neighboring populations and formed the current East-West Eurasian admixed genetic profile after their separation from the Mongolians.
- Published
- 2019
7. Expression profile analysis of piwi-interacting RNA in forensically relevant biological fluids
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Guanglin He, Xing Zou, Jing Liu, Ruiyang Tao, Mengge Wang, Yiwen Yang, Shouyu Wang, Yiping Hou, Mingkun Xie, and Zheng Wang
- Subjects
Forensic Genetics ,Genetic Markers ,Male ,0301 basic medicine ,endocrine system ,Small RNA ,Piwi-interacting RNA ,Computational biology ,Biology ,Real-Time Polymerase Chain Reaction ,Pathology and Forensic Medicine ,03 medical and health sciences ,0302 clinical medicine ,Semen ,Genetics ,TaqMan ,Biological fluids ,Humans ,Profile analysis ,030216 legal & forensic medicine ,RNA, Small Interfering ,Saliva ,Skin ,Massive parallel sequencing ,Sequence Analysis, RNA ,urogenital system ,Discriminant Analysis ,High-Throughput Nucleotide Sequencing ,RNA ,Menstruation ,Hierarchical clustering ,030104 developmental biology ,Cervix Mucus ,Female ,Blood Chemical Analysis - Abstract
During the last decade, RNA profiling has emerged as one of the fastest developed methods for discriminating forensically relevant biological samples. As a category of small non-coding RNA, piwi-interacting RNA (piRNA) has recently been proposed to be differentially expressed in different types of body fluids, which indicates that its potential in forensic science is worth exploring. In this study, small RNA from 6 types of biological samples (venous blood, menstrual blood, saliva, semen, vaginal secretions and skin) was prepared and sequenced in order to characterize the expression pattern of piRNA using Ion S5 XL platform. Multiple bioinformatic methods were applied to make interpretation of the massively parallel sequencing data and identify representative biomarkers. A total of 376 piRNAs were initially identified after normalization and filtering. Hierarchical clustering and partial least squares-discriminant analysis (PLS-DA) revealed that their expression profiles exhibited an acceptable discriminating ability for most biological samples. Besides, a panel consists of 37 piRNA candidates was subsequently established for further analysis. The results suggested that with the optimal number of PLS components, the marker-reduced panel was sufficient to construct a PLS-DA model with the same performance as that can be achieved by the entire 376 piRNAs (classification error rate = 0.04). In addition, 5 targeted candidates were further selected for validation. TaqMan RT-qPCR assay results verified the potential of 3 piRNAs (piR-hsa-27622, piR-hsa-1207 and piR-hsa-27493) in distinguishing venous blood and menstrual blood, as well as 2 piRNA (piR-hsa-27493 and piR-hsa-26591) for the discrimination of saliva and vaginal secretions, which emphasized the feasibility of our biomarker selection approach. In brief, our study expanded the amount of potential piRNA biomarkers and demonstrated that the expression features of piRNA could provide valuable information for discriminating forensically relevant biological samples.
- Published
- 2019
8. Tai-Kadai-speaking Gelao population: Forensic features, genetic diversity and population structure
- Author
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Yiping Hou, Guanglin He, Shouyu Wang, Xing Zou, Jing Liu, Zheng Wang, Pengyu Chen, Mengge Wang, and Ziwei Ye
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Male ,0301 basic medicine ,China ,Genotype ,Genetic genealogy ,Population ,Population genetics ,Biology ,Population stratification ,Polymerase Chain Reaction ,Pathology and Forensic Medicine ,03 medical and health sciences ,0302 clinical medicine ,Gene Frequency ,INDEL Mutation ,Genetic variation ,Ethnicity ,Genetics ,Humans ,030216 legal & forensic medicine ,Indel ,education ,Allele frequency ,education.field_of_study ,Genetic diversity ,Genetic Variation ,DNA Fingerprinting ,Genetics, Population ,030104 developmental biology ,Evolutionary biology ,Female ,Microsatellite Repeats - Abstract
Genetic analyses of geographically and ethno-linguistically different populations are essential for understanding population stratification and genomic structure in medical Genome-Wide Association Studies (GWAS) and genetic variation and diversity related to forensic and population genetics studies. Here, we genotyped 30 autosomal insertion/deletion (Indel) markers from 502 Tai-Kadai-speaking Gelao individuals residing in the rugged topographical area in Southeastern China. In addition, two comprehensive population genetic comparisons of 15,327 individuals from 95 worldwide populations and of 6122 individuals from Asia and adjoining populations were conducted based on allele frequency data and raw genotype data, respectively. All studied markers were found to be in Hardy-Weinberg equilibrium. The combined power of discrimination in the Gelao minority group was 0.999999999975, and the combined probability of exclusion was 0.9879. Our results from the forensic statistical parameters indicated that this Indel panel can be independently used as a powerful tool in forensic individual identification but can only be used as a complementary tool in paternity cases involving East Asians. We also found significant allele frequency differences between the Gelao and other continental populations with respect to the markers grouped in clusters ∼Ⅳ, suggesting that these can be used as forensic ancestry informative Indel markers to distinguish the Gelao from other continental populations. Genetic ancestry analyses demonstrated that Tai-Kadai-speaking Gelao share a dominant ancestry component with Hmong-Mien-speaking Miao. Our population genetic results from multidimensional scaling plots, principal component analysis, neighboring-joining tree construction and hierarchical clustering also suggested that the Zunyi Gelao are genetically closer to their linguistically or geographically close populations, such as the Han Chinese, Guizhou Bouyei and the Hubei Tujia, than to Turkic and Tibeto-Burman speakers.
- Published
- 2019
9. The potential use of Piwi-interacting RNA biomarkers in forensic body fluid identification: A proof-of-principle study
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Ruiyang Tao, Chengtao Li, Yiping Hou, Jing Liu, Zheng Wang, Guanglin He, and Shouyu Wang
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Adult ,Forensic Genetics ,Genetic Markers ,Male ,0301 basic medicine ,endocrine system ,Piwi-interacting RNA ,Semen ,Biology ,Real-Time Polymerase Chain Reaction ,Pathology and Forensic Medicine ,Young Adult ,03 medical and health sciences ,0302 clinical medicine ,Circular RNA ,microRNA ,Genetics ,TaqMan ,Humans ,030216 legal & forensic medicine ,RNA, Small Interfering ,Saliva ,Body fluid ,Messenger RNA ,urogenital system ,RNA ,Menstruation ,030104 developmental biology ,Biochemistry ,Cervix Mucus ,Female ,Blood Chemical Analysis - Abstract
In the forensic community, RNA profiling has been investigated as a potential method to identify body fluids. Several RNA molecules, including messenger RNA (mRNA), microRNA (miRNA) and circular RNA (circRNA), have been explored as biomarkers to distinguish different body fluids and have led to considerable interest in the development of RNA biomarkers for forensic purposes. Piwi-interacting RNA (piRNA), a class of noncoding RNAs, is a potential biomarker for body fluid identification because of its short length (˜24–32 nt) and specific expression pattern in human tissues. In this proof-of-principle study, we examined the expression levels of four carefully selected piRNAs in forensically relevant biological fluids (venous blood, saliva, semen, menstrual blood and vaginal secretions) using TaqMan quantitative real-time polymerase chain reaction (TaqMan qPCR). piR-55521, which was not detectable in saliva, can differentiate semen from other body fluids because it was strongly expressed in semen compared to the remaining three fluids (> 4000-fold change). Furthermore, piR-55521 could be detected in semen samples made from as little as 200 pg of total RNA, and addition of female component had no effect on the detection limit. Furthermore, the expression differences of other piRNAs, piR-61648, piR-43994 and piR-33151, were statistically significant between at least two types of body fluids. Stability tests also indicated that these piRNAs could be effectively detected in dried samples under laboratory and outdoor conditions for at least six months. Although limited to four piRNAs, this study suggests that the expression pattern of piRNAs could be used to identify body fluids, and that piRNA (piR-55521) is specifically expressed in semen. Such findings suggest that additional work could identify other piRNAs that could serve as biomarkers to identify body fluids.
- Published
- 2019
10. Developmental validation of a custom panel including 165 Y-SNPs for Chinese Y-chromosomal haplogroups dissection using the ion S5 XL system
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Min Lang, Mengge Wang, Guanglin He, Jienan Li, Yiping Hou, Shouyu Wang, Mingkun Xie, Chengtao Li, Jing Liu, Xiaoqing Qian, and Zheng Wang
- Subjects
Forensic Genetics ,Male ,0301 basic medicine ,Lineage (genetic) ,Single-nucleotide polymorphism ,Computational biology ,Biology ,Polymorphism, Single Nucleotide ,Haplogroup ,Pathology and Forensic Medicine ,03 medical and health sciences ,0302 clinical medicine ,Asian People ,Genotype ,Genetics ,Humans ,030216 legal & forensic medicine ,Y-SNP ,Chromosomes, Human, Y ,Massive parallel sequencing ,High-Throughput Nucleotide Sequencing ,Reproducibility of Results ,Sequence Analysis, DNA ,030104 developmental biology ,Pyrosequencing ,SNP array - Abstract
Massively parallel sequencing (MPS) technology has the capability to genotype hundreds to thousands of SNPs from multiple samples, with high coverage in a single experimental run. MPS, which enables high-resolution Y haplogrouping from limited DNA resources, has attracted much interest in Y-chromosomal applications, particularly human forensics and anthropological studies. Previously, we selected and designed a 72 Y-SNPs MPS panel aiming at the Chinese population for providing the basic structure of the haplogroup tree, and then additional Y-SNPs were screened out for further subdivision into different populations by using pyrosequencing technology. In the present study, we designed a customized MPS panel that includes 165 Y-SNPs based on our previous studies and evaluated the sequencing performance, sensitivity and the ability of the MPS-SNP panel to analyze case-type samples using the Ion S5 XL System. In the concordant study, only four Y-SNPs presented missing genotypes due to low coverage reads, and five SNPs were prone to being designated heterozygotes, whereas the designation of the other SNPs were fully consistent with the results obtained from other Y-SNP typing tools. The analyst-interpreted Y-SNP profiles demonstrated that the targeted Y-SNPs, except for the nine poorly performing Y-SNPs, performed well and could acquire achievable high-resolution. Moreover, this MPS panel could be adapted to case-type samples, and single source complete profiles could be stably obtained using 1 ng of input DNA. In summary, this MPS Y-SNP panel offers a straightforward sample-to-haplogroup workflow that would be beneficial for paternal lineage classification and forensic pedigree searches, and the present results provide support for the application of MPS technology in SNP analysis.
- Published
- 2019
11. Genetic polymorphisms for 19 X-STR loci of Sichuan Han ethnicity and its comparison with Chinese populations
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Xing Zou, Guanglin He, Jin Wu, Miao Liao, Ye Li, Pengyu Chen, and Mengge Wang
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Male ,0301 basic medicine ,China ,Population ,Locus (genetics) ,Biology ,Pathology and Forensic Medicine ,03 medical and health sciences ,0302 clinical medicine ,Gene Frequency ,Genetic variation ,Ethnicity ,Humans ,030216 legal & forensic medicine ,Allele ,education ,Allele frequency ,Genetics ,Chromosomes, Human, X ,education.field_of_study ,Polymorphism, Genetic ,Genetic heterogeneity ,Haplotype ,Issues, ethics and legal aspects ,Genetics, Population ,030104 developmental biology ,Microsatellite ,Female ,Microsatellite Repeats - Abstract
X-chromosomal short tandem repeats (X-STRs) can be serviced as a complementary tool in forensic deficiency cases and other complicated kinship identification. To investigate the genetic variation of the western Chinese Han population, genetic polymorphisms of 19 X-STR loci (DXS8378, DXS7423, DXS10148, DXS10159, DXS10134, DXS7424, DXS10164, DXS10162, DXS7132, DXS10079, DXS6789, DXS101, DXS10103, DXS10101, HPRTB, DXS6809, DXS10075, DXS10074 and DXS10135) included in the AGCU X19 PCR amplification kit were obtained from 201 Chinese Han individuals (108 females and 93 males) residing in Sichuan province, western China. A total of 211 alleles were found from the 19 X-STR loci, and 4-22 alleles were found for each locus with the corresponding allele frequencies spanned from 0.0034 to 0.6395. The combined PDF and PDM were 0.9999999999999999999992 and 0.9999999999997, respectively. The combined MECKruger, MECKishida, MECDesmarais as well as MECDesmarais Duo were 0.999999958110254, 0.999999999996533, 0.999999999996695 and 0.999999992428394, respectively. The number of haplotypes observed in the seven linkage groups varied from 27 to 97 for 108 male individuals with the corresponding haplotype diversities spanned from 0.9424 (DXS7424-DXS101) to 0.9979 (DXS7132-DXS10079-DXS10075-DXS10074). The above-mentioned results indicated that the 19 X-STR loci and the seven linkage groups are highly polymorphic and informative in the Sichuan Han population. Results from population comparisons and phylogenetic analyses demonstrated that genetic heterogeneity widely existed between the Sichuan Han population and Xinjiang minority ethnic groups (Uyghur and Kazakh) and genetic affinity was identified in populations which possessed the same ethno-origin or close geographic origin.
- Published
- 2017
12. Genetic insights into the paternal admixture history of Chinese Mongolians via high-resolution customized Y-SNP SNaPshot panels
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Xing Zou, Mengge Wang, Weian Du, Jing Liu, Yiping Hou, Tianyue Ming, Zheng Wang, Ziwei Ye, and Guanglin He
- Subjects
China ,education.field_of_study ,Chromosomes, Human, Y ,Lineage (genetic) ,Population ,Haplotype ,Polymorphism, Single Nucleotide ,Haplogroup ,Pathology and Forensic Medicine ,Genetics, Population ,Geography ,Ancient DNA ,Haplotypes ,Evolutionary biology ,Genetic structure ,Ethnicity ,Genetics ,Humans ,Microsatellite ,education ,Y-SNP ,Language ,Microsatellite Repeats - Abstract
The Mongolian people, one of the Mongolic-speaking populations, are native to the Mongolian Plateau in North China and southern Siberia. Many ancient DNA studies recently reported extensive population transformations during the Paleolithic to historic periods in this region, while little is known about the paternal genetic legacy of modern geographically different Mongolians. Here, we genotyped 215 Y-chromosomal single nucleotide polymorphisms (Y-SNPs) and 37 Y-chromosomal short tandem repeats (Y-STRs) among 679 Mongolian individuals from Hohhot, Hulunbuir, and Ordos in North China using the AGCU Y37 kit and our developed eight Y-SNP SNaPshot panels (including two panels first reported herein). The C-M130 Y-SNP SNaPshot panel defines 28 subhaplogroups, and the N/O/Q complementary Y-SNP SNaPshot panel defines 30 subhaplogroups of N1b-F2930, N1a1a1a1a3-B197, Q-M242, and O2a2b1a1a1a4a-CTS4658, which improved the resolution our developed Y-SNP SNaPshot panel set and could be applied for dissecting the finer-scale paternal lineages of Mongolic speakers. We found a strong association between Mongolian-prevailing haplogroups and some observed microvariants among the newly generated Y-STR haplotype data, suggesting the possibility of haplogroup prediction based on the distribution of Y-STR haplotypes. We identified three main ancestral sources of the observed Mongolian-dominant haplogroups, including the local lineage of C2*-M217 and incoming lineages from other regions of southern East Asia (O2*-M122, O1b*-P31, and N1*-CTS3750) and western Eurasia (R1*-M173). We also observed DE-M145, D1*-M174, C1*-F3393, G*-M201, I-M170, J*-M304, L-M20, O1a*-M119, and Q*-M242 at relatively low frequencies (< 5.00%), suggesting a complex admixture history between Mongolians and other incoming Eurasians from surrounding regions. Genetic clustering analyses indicated that the studied Mongolians showed close genetic affinities with other Altaic-speaking populations and Sinitic-speaking Hui people. The Y-SNP haplotype/haplogroup-based genetic legacy not only revealed that the stratification among geographically/linguistically/ethnically different Chinese populations was highly consistent with the geographical division and language classification, but also demonstrated that patrilineal genetic materials could provide fine-scale genetic structures among geographically different Mongolian people, suggesting that our developed high-resolution Y-SNP SNaPshot panels have the potential for forensic pedigree searches and biogeographical ancestry inference.
- Published
- 2021
13. Massively parallel sequencing of mitogenome sequences reveals the forensic features and maternal diversity of tai-kadai-speaking hlai islanders
- Author
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Mengge, Wang, primary, Guanglin, He, additional, Yongdong, Su, additional, Shouyu, Wang, additional, Xing, Zou, additional, Jing, Liu, additional, Zheng, Wang, additional, and Hou, Yiping, additional
- Published
- 2020
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14. Massively parallel sequencing of 165 ancestry-informative SNPs and forensic biogeographical ancestry inference in three southern Chinese Sinitic/Tai-Kadai populations
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Zheng Wang, Sumin Zhu, Hui-Yuan Yeh, Guanglin He, Yiping Hou, Tianyue Ming, Chuan-Chao Wang, Xing Zou, Jing Liu, and Mengge Wang
- Subjects
0301 basic medicine ,China ,Population ,Ethnic group ,Genetic admixture ,Ancestry-informative marker ,Polymorphism, Single Nucleotide ,Pathology and Forensic Medicine ,03 medical and health sciences ,0302 clinical medicine ,Ethnicity ,Genetics ,Humans ,030216 legal & forensic medicine ,1000 Genomes Project ,education ,Allele frequency ,education.field_of_study ,Phylogenetic tree ,High-Throughput Nucleotide Sequencing ,Sequence Analysis, DNA ,DNA Fingerprinting ,Phylogeography ,Genetics, Population ,030104 developmental biology ,Geography ,Evolutionary biology ,Identification (biology) ,Microsatellite Repeats - Abstract
Ancestry informative markers (AIMs), which are distributed throughout the human genome, harbor significant allele frequency differences among diverse ethnic groups. The use of sets of AIMs to reconstruct population history and genetic relationships is attracting interest in the forensic community, because biogeographic ancestry information for a casework sample can potentially be predicted and used to guide the investigative process. However, subpopulation ancestry inference within East Asia remains in its infancy due to a lack of population reference data collection and incomplete validation work on newly developed or commercial AIM sets. In the present study, 316 Chinese persons, including 85 Sinitic-speaking Haikou Han, 120 Qiongzhong Hlai and 111 Daozhen Gelao individuals belonging to Tai-Kadai-speaking populations, were analyzed using the Precision ID Ancestry Panel (165 AISNPs). Combined with our previous 165-AISNP data (375 individuals from 6 populations), the 1000 Genomes Project and forensic literature, comprehensive population genetic comparisons and ancestry inference were further performed via ADMIXTURE, TreeMix, PCA, f-statistics and N-J tree. Although several nonpolymorphic loci were identified in the three southern Chinese populations, the forensic parameters of this ancestry inference panel were better than those for the 23 STR-based Huaxia Platinum System, which is suitable for use as a robust tool in forensic individual identification and parentage testing. The results based on the ancestry assignment and admixture proportion evaluation revealed that this panel could be used successfully to assign individuals at a continental scale but also possessed obvious limitations in discriminatory power in intercontinental individuals, especially for European-Asian admixed Uyghurs or in populations lacking reference databases. Population genetic analyses further revealed five continental population clusters and three East Asian-focused population subgroups, which is consistent with linguistic affiliations. Ancestry composition and multiple phylogenetic analysis further demonstrated that the geographically isolated Qiongzhong Hlai harbored a close phylogenetic relationship with Austronesian speakers and possessed a homogenous Tai-Kadai-dominant ancestry, which could be used as the ancestral source proxy in population history reconstruction of Tai-Kadai-speaking populations and as one of the representatives for forensic database establishment. In summary, more population-specific AIM sets focused on East Asian subpopulations, comprehensive algorithms and high-coverage population reference data should be developed and validated in the next step.
- Published
- 2021
15. Forensic characteristics and phylogenetic analysis of Hubei Han population in central China using 17 Y-STR loci
- Author
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Weian Du, Guanglin He, Zheng Wang, Jing Liu, and Yiping Hou
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Male ,0301 basic medicine ,Genetics ,China ,Chromosomes, Human, Y ,Genotype ,Phylogenetic tree ,Haplotype ,Biology ,DNA Fingerprinting ,Analysis of molecular variance ,Pathology and Forensic Medicine ,03 medical and health sciences ,Genetics, Population ,030104 developmental biology ,Haplotypes ,Ethnicity ,Humans ,Y-STR ,Allele ,Allele frequency ,Microsatellite Repeats - Abstract
Currently, the largest national database within the Y chromosome haplotype reference database (YHRD, https://yhrd.org, release 53) is China, which has approximately 38000 Y chromosomal 17-marker (Yfiler) haplotypes. These haplotype profiles derived from the vast majority of Chinese administrative divisions, but no haplotype data was available for Hubei province, which is located in the Central China region. Herein, 429 unrelated male Chinese Han individuals residing in Hubei province were recruited and genotyped with 17 Y-STR loci. 115 alleles were identified with corresponding allele frequencies spanned from 0.0023 to 07506. The gene diversity (GD) values ranged from 0.3988 at DYS438 to 0.9573 at DYS385a/b. A total of 410 distinct haplotypes were obtained with the overall haplotype diversity (HD) and discrimination capacity (DC) was 0.9995 and 0.9557, respectively. Additionally, genetic relationships along administrative (Han Chinese from different provinces) and ethnic divisions (minority ethnic groups) were analyzed using analysis of molecular variance (AMOVA) tests and visualized by multidimensional scaling plots (MDS). The Han ethnicity including the Hubei Han shows a high genetic homogeneity all across China and significant genetic differences existed between the Hubei Han and some ethnic groups, most prominently for the Kazakhs and the Tibetans.
- Published
- 2017
16. Forensic characteristics of Tibeto-Burman-speaking Tibetans revealed by 50 InDels
- Author
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Hong Zhu, Guanglin He, Mengge Wang, Yiping Hou, and Zheng Wang
- Subjects
010401 analytical chemistry ,food and beverages ,Biology ,01 natural sciences ,0104 chemical sciences ,Pathology and Forensic Medicine ,Forensic science ,Loss of heterozygosity ,03 medical and health sciences ,symbols.namesake ,0302 clinical medicine ,Bonferroni correction ,Evolutionary biology ,Genetics ,symbols ,030216 legal & forensic medicine ,Allele ,Indel ,Allele frequency ,Forensic genetics - Abstract
Insertion/deletion polymorphism (InDel) profiling has become a promising tool in forensic investigations and biogeographical ancestry inferences. Recently, the AGCU has designed an advanced InDel assay that allows co-amplification and fluorescent detection of the 47 autosomal InDels (A-InDels), 2 Y-chromosomal InDels (Y-InDels) and Amelogenin. In this study, 103 Tibeto-Burman-speaking Tibetan individuals were genotyped to investigate the effectiveness of this novel InDel kit. No significant deviation from Hardy-Weinberg equilibrium was observed at the 47 A-InDels after applying the Bonferroni correction. The allelic frequencies of insertion alleles at the 47 A-InDels ranged from 0.2087 to 0.7767, and the mean observed heterozygosity varied from 0.3010 to 0.5922. The combined power of discrimination reached 1–4.0688E-19, and the combined probability of exclusion was 0.9997. Overall, the observed results demonstrate that this novel InDel assay can be used as an efficient tool for individual identification and forensic paternity testing.
- Published
- 2019
17. Genetic diversity of 21 autosomal STR loci in the Han population from Sichuan province, Southwest China
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Zheng Wang, Ji Zhang, Yingbi Li, Haibo Luo, Jin Wu, Miao Liao, Weibo Liang, Yiping Hou, Ye Li, Guanglin He, and Jing Yan
- Subjects
0301 basic medicine ,China ,Population ,Population genetics ,Genetic relationship ,Ethnic origin ,Polymerase Chain Reaction ,Pathology and Forensic Medicine ,03 medical and health sciences ,Gene Frequency ,Ethnicity ,Genetics ,Humans ,Han population ,education ,Genetic diversity ,education.field_of_study ,Polymorphism, Genetic ,Phylogenetic tree ,DNA Fingerprinting ,Genetics, Population ,030104 developmental biology ,Geography ,Evolutionary biology ,Microsatellite Repeats - Abstract
Exploration of the ethnic origin and genetic differentiation of 56 Chinese officially recognized nationalities populations played a fundamental role in the research field of population genetics, forensic science, linguistics, anthropology, and archaeology. In the present study, population data of 21 autosomal STR loci (CSF1PO, D10S1248, D12S391, D13S317, D16S539, D18S51, D19S433, D21S11, D2S1338, D2S441, D3S1358, D5S818, D6S1043, D7S820, D8S1179, FGA, Penta D, Penta E, TH01, TPOX, and vWA) included in the AGCU EX22 kit in 2793 Southwest Han Chinese individuals was obtained and population genetic relationships among 28 Chinese populations were investigated. Our study indicated that the twenty-one autosomal STRs are highly polymorphic in the Sichuan Han population and can be used as a powerful tool in the routine forensic usage. MDS and phylogenetic analysis suggested that the Sichuan Han population kept a close genetic relationship with the southwest populations.
- Published
- 2017
18. Association between interleukin-18 promoter genetic polymorphisms, haplotypes and allergic asthma susceptibility
- Author
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Pengyu Chen, Guanglin He, Jing Yan, Tianzhen Gao, Xing Zou, and Xu Chen
- Subjects
0301 basic medicine ,Genetics ,Haplotype ,Single-nucleotide polymorphism ,Promoter ,Odds ratio ,Biology ,medicine.disease ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Polymorphism (computer science) ,Immunology ,Genetic model ,Genotype ,medicine ,Genetics (clinical) ,030215 immunology ,Asthma - Abstract
Numerous studies have investigated the association between the polymorphisms or haplotypes in interleukin-18 (IL-18) gene promoter and allergic asthma susceptibility, however, the conclusions were controversial. Thus, we performed this comprehensive meta-analysis to evaluate this correlation. Potentially eligible studies were searched in the common electronic database. A total of nine studies for rs1946518, ten studies for rs187238 as well as four studies for haplotypes of the IL-18 promoter were included. Pooled odds ratios (ORs) and 95% confidence intervals (CIs) were implemented to assess the strength of the association between polymorphisms or haplotypes and the risk of asthma. Our meta-analysis revealed that a possible association with asthma susceptibility was identified with borderline significance in the overall analysis under dominant genetic model and heterozygous model for IL-18-607 C/A Polymorphism, under the homozygous genetic model for IL-18-137 G/C polymorphism and haplotype of CC in IL-18 gene promoter. Our meta-analysis indicated that genotype AA and CA in rs1946518 may be a protective role in allergic asthma progress, while carriers of genotype GG in rs187238 may be associated with increased allergic asthma risk. Besides, haplotype of CC in the IL-18 gene promoter may be also associated with allergic asthma susceptibility.
- Published
- 2017
19. Forensic features, genetic diversity and structure analysis of three Chinese populations using 47 autosomal InDels
- Author
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Jing Liu, Weian Du, Mengge Wang, Guanglin He, Changhui Liu, Zheng Wang, and Shouyu Wang
- Subjects
Genetic Markers ,0301 basic medicine ,China ,Population genetics ,Genetic relationship ,Biology ,Polymerase Chain Reaction ,Pathology and Forensic Medicine ,03 medical and health sciences ,0302 clinical medicine ,Asian People ,Gene Frequency ,INDEL Mutation ,Ethnicity ,Genetics ,Humans ,030216 legal & forensic medicine ,Indel ,Phylogeny ,Principal Component Analysis ,Genetic diversity ,Polymorphism, Genetic ,Phylogenetic tree ,food and beverages ,Genetics, Population ,030104 developmental biology ,Evolutionary biology ,Genetic marker ,Genetic structure ,Microsatellite - Abstract
Insertion/deletion polymorphisms (InDels), which combine the desirable features of both short tandem repeats (STRs) and single-nucleotide polymorphisms (SNPs), have become widely used genetic markers for forensic investigations, anthropology and population genetics. The AGCU InDel 50 kit is a newly developed panel that contains 47 autosomal InDels (A-InDels), 2 Y-chromosomal InDels (Y-InDels) and Amelogenin and is designed to provide a higher discriminatory power in Chinese populations compared with the Qiagen DIPplex kit. In this study, 542 unrelated individuals were first genotyped to evaluate the forensic efficiency of this novel panel in three Chinese ethnicities (Hainan Han, Hainan Li and Zunyi Gelao groups). Additionally, genetic relationships among the three investigated populations (geographically close but linguistically different populations: Han and Li; geographically diverse but from the same language family: Li and Gelao) and 31 worldwide populations were analyzed using pairwise genetic distances, multidimensional scaling (MDS), phylogenetic tree, principal component analysis (PCA) and STRUCTURE. The combined powers of discrimination (CPD) for the Han, Li and Gelao groups were 0.999999999999999999635, 0.999999999999999997668 and 0.999999999999999999840, respectively, and the combined powers of exclusion (CPE) were 0.999715, 0.999283 and 0.999575, respectively. The genetic relationship between the Hainan Han and Zunyi Gelao groups was relatively closer than that between the Hainan Li and Zunyi Gelao groups, demonstrating that there was little gene communication between Li and Han living on Hainan Island as well as between Li and Gelao in the Tai-Kadai language family. The aforementioned results suggest that the AGCU InDel 50 kit is an effective tool that is appropriate for personal identification and population genetics.
- Published
- 2020
20. Whole mitochondrial genome analysis of highland Tibetan ethnicity using massively parallel sequencing
- Author
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Shouyu Wang, Xing Zou, Jing Liu, Yiping Hou, Zheng Wang, Guanglin He, Fei Wang, Mengge Wang, and Ziwei Ye
- Subjects
Male ,0301 basic medicine ,Mitochondrial DNA ,Population ,Datasets as Topic ,Biology ,Tibet ,DNA, Mitochondrial ,Genome ,Haplogroup ,Pathology and Forensic Medicine ,03 medical and health sciences ,0302 clinical medicine ,Ethnicity ,Genetics ,Humans ,030216 legal & forensic medicine ,education ,Phylogeny ,education.field_of_study ,Massive parallel sequencing ,Haplotype ,High-Throughput Nucleotide Sequencing ,Maximum parsimony ,Genetics, Population ,030104 developmental biology ,Haplotypes ,Evolutionary biology ,Genome, Mitochondrial ,Female ,Gene pool - Abstract
Mitochondrial DNA (mtDNA) is a key player in numerous multifaceted and intricate biological processes and plays a pivotal role in dissecting the peopling of different populations, due to its maternally inherited property and comparatively high mutation rate. In this study, 119 Tibetan individuals from the Muli Tibetan Autonomous County of China (average altitude above 3,000 m) were employed in mitochondrial genome (mitogenome) sequencing by massively parallel sequencing (MPS) techniques using the Precision ID mtDNA Whole Genome Panel on an Ion S5XL system. The dataset presented 88 distinct haplotypes, resulting in the haplotype diversity of 0.9909. The majority of haplotypes were assigned to East Asian lineages and the distribution of haplogroups of Muli Tibetan significantly differed from reference Tibetan populations. The maximum parsimony phylogeny reconstructed by 119 newly generated mitogenomes revealed 12 major Muli Tibetan lineages. Intriguingly, a Sherpa-specific sub-haplogroup A15c1 with the lack of mutations at 4216 and 15,924 was discerned in our dataset, which suggested that the maternal gene pool of Sherpas may derive from Tibetan populations. The shared haplogroups between Muli Tibetan and lowland Han Chinese hinted that these lineages may derive from non-Tibetans and have already differentiated before their arrival on the Tibetan Plateau. Furthermore, extensive pairwise population comparisons displayed that Muli Tibetan had a closer genetic relationship with ethnically or linguistically close Nyingtri Tibetan, Nyingtri Lhoba and Chamdo Tibetan populations. Genetic affinity was also observed between the Muli Tibetan and North Han Chinese. Collectively, the results generated in this study enriched the existing forensic mtDNA database and raised additional interest in the application of whole mitogenome sequencing in forensic investigations.
- Published
- 2020
21. Chinese population genetic substructure using 23 Y-chromosomal STRs
- Author
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Yiping Hou, Guanglin He, Jing Yan, and Zheng Wang
- Subjects
0301 basic medicine ,Genetics ,Phylogenetic tree ,Haplotype ,Biology ,Y chromosome ,Mega ,Analysis of molecular variance ,Pathology and Forensic Medicine ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Molecular anthropology ,Genetic distance ,030216 legal & forensic medicine ,Multidimensional scaling - Abstract
China, as the largest national database within the YHRD (Y chromosome haplotype reference database), has repeatedly been a research hotspot in the field of genetics, archaeology and molecular anthropology. In the present study, 23 Y-STR loci were used to investigate genetic relationships among 29 Chinese populations (6029 individuals). Pairwise genetic distance (R st value) was calculated based on haplotype frequency distributions by the Analysis of Molecular Variance. Multidimensional scaling (MDS) and phylogenetic analysis were performed on the online tool and Mega 6.0, respectively. Tibetans (R st =0.1601±0.0421), Shuis (R st =0.0934±0.0327), Uyghurs (R st =0.0791±0.0459) and Zhuangs (R st =0.0790±0.0404) showed the high genetic distance with other populations and kept relatively isolated with other reference populations in the MDS plots and Neighbor-Joining tree. Additionally, genetic distinction among same ethnicities (Han and Hui) distributed in different administrative divisions was also explored and no significant differences were identified.
- Published
- 2017
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