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Your search keyword '"Freudenberg, Uwe"' showing total 30 results
Start Over Author "Freudenberg, Uwe" Publisher elsevier bv
30 results on '"Freudenberg, Uwe"'

4. 3D Culture Method for Alzheimer's Disease Modeling Reveals Interleukin-4 Rescues Aβ42-Induced Loss of Human Neural Stem Cell Plasticity

Author

Papadimitriou, Christos, primary, Celikkaya, Hilal, additional, Cosacak, Mehmet I., additional, Mashkaryan, Violeta, additional, Bray, Laura, additional, Bhattarai, Prabesh, additional, Brandt, Kerstin, additional, Hollak, Heike, additional, Chen, Xin, additional, He, Shuijin, additional, Antos, Christopher L., additional, Lin, Weilin, additional, Thomas, Alvin Kuriakose, additional, Dahl, Andreas, additional, Kurth, Thomas, additional, Friedrichs, Jens, additional, Zhang, Yixin, additional, Freudenberg, Uwe, additional, Werner, Carsten, additional, and Kizil, Caghan, additional

Published
2018
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9. Cell-instructive starPEG-heparin-collagen composite matrices

Author

Binner, Marcus, Bray, Laura, Friedrichs, Jens, Freudenberg, Uwe, Tsurkan, Mikhail, Werner, Carsten, Binner, Marcus, Bray, Laura, Friedrichs, Jens, Freudenberg, Uwe, Tsurkan, Mikhail, and Werner, Carsten

Abstract

Polymer hydrogels can be readily modulated with regard to their physical properties and functionalized to recapitulate molecular cues of the extracellular matrix (ECM). However, they remain structurally different from the hierarchical supramolecular assemblies of natural ECM. Accordingly, we herein report a set of hydrogel composite materials made from starPEG-peptide conjugates, maleimide-functionalized heparin and collagen type I that combine semisynthetic and ECM-derived components. Collagen fibrillogenesis was controlled by temperature and collagen concentration to form collagen microstructures which were then homogeneously distributed within the 3D composite matrix during hydrogel formation. The collagen-laden hydrogel materials showed a heterogeneous local variation of the stiffness and adhesion ligand density. Composite gels functionalized with growth factors and cell adhesive peptides (RGDSP) supported the growth of embedded human umbilical cord vein endothelial cells (HUVECs) and induced the alignment of embedded bone marrow–derived human mesenchymal stem cells (MSCs) to the collagen microstructures in vitro. The introduced composite hydrogel material is concluded to faithfully mimic cell-instructive features of the ECM. Statement of significant Cell-instructive materials play an important role in the generation of both regenerative therapies and advanced tissue and disease models. For that purpose, biofunctional polymer hydrogels recapitulating molecular cues of the extracellular matrix (ECM) were successfully applied in various different studies. However, hydrogels generally lack the hierarchical supramolecular structure of natural ECM. We have therefore developed a hydrogel composite material made from starPEG-peptide conjugates, maleimide-functionalized heparin and collagen type I fibrils. The collagen-laden scaffolds showed a heterogeneous local variation in the stiffness of the material. The composite gels were successfully tested in culture ex

Published
2017

11. 3D extracellular matrix interactions modulate tumour cell growth, invasion and angiogenesis in engineered tumour microenvironments

Author

Taubenberger, Anna, Bray, Laura, Haller, Barbara, Shaposhnykov, Artem, Binner, Marcus, Freudenberg, Uwe, Guck, Jochen, Werner, Carsten, Taubenberger, Anna, Bray, Laura, Haller, Barbara, Shaposhnykov, Artem, Binner, Marcus, Freudenberg, Uwe, Guck, Jochen, and Werner, Carsten

Abstract

Interactions between tumour cells and extracellular matrix proteins of the tumour microenvironment play crucial roles in cancer progression. So far, however, there are only a few experimental platforms available that allow us to study these interactions systematically in a mechanically defined three-dimensional (3D) context. Here, we have studied the effect of integrin binding motifs found within common extracellular matrix (ECM) proteins on 3D breast (MCF-7) and prostate (PC-3, LNCaP) cancer cell cultures, and co-cultures with endothelial and mesenchymal stromal cells. For this purpose, matrix metalloproteinase-degradable biohybrid poly(ethylene) glycol-heparin hydrogels were decorated with the peptide motifs RGD, GFOGER (collagen I), or IKVAV (laminin-111). Over 14 days, cancer spheroids of 100-200µm formed. While the morphology of poorly invasive MCF-7 and LNCaP cells was not modulated by any of the peptide motifs, the aggressive PC-3 cells exhibited an invasive morphology when cultured in hydrogels comprising IKVAV and GFOGER motifs compared to RGD motifs or nonfunctionalised controls. PC-3 (but not MCF-7 and LNCaP) cell growth and endothelial cell infiltration were also significantly enhanced in IKVAV and GFOGER presenting gels. Taken together, we have established a 3D culture model that allows for dissecting the effect of biochemical cues on processes relevant to early cancer progression. These findings provide a basis for more mechanistic studies that may further advance our understanding of how ECM modulates cancer cell invasion and how to ultimately interfere with this process.

Published
2016

12. Macroporous biohybrid cryogels for co-housing pancreatic islets with mesenchymal stromal cells

Author

Borg, Danielle J., primary, Welzel, Petra B., additional, Grimmer, Milauscha, additional, Friedrichs, Jens, additional, Weigelt, Marc, additional, Wilhelm, Carmen, additional, Prewitz, Marina, additional, Stißel, Aline, additional, Hommel, Angela, additional, Kurth, Thomas, additional, Freudenberg, Uwe, additional, Bonifacio, Ezio, additional, and Werner, Carsten, additional

Published
2016
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14. Multi-parametric hydrogels support 3D in vitro bioengineered microenvironment models of tumour angiogenesis

Author

Bray, Laura, Binner, Marcus, Holzheu, Anja, Friedrichs, Jens, Freudenberg, Uwe, Hutmacher, Dietmar, Werner, Carsten, Bray, Laura, Binner, Marcus, Holzheu, Anja, Friedrichs, Jens, Freudenberg, Uwe, Hutmacher, Dietmar, and Werner, Carsten

Abstract

Tumour microenvironment greatly influences the development and metastasis of cancer progression. The development of three dimensional (3D) culture models which mimic that displayed in vivo can improve cancer biology studies and accelerate novel anticancer drug screening. Inspired by a systems biology approach, we have formed 3D in vitro bioengineered tumour angiogenesis microenvironments within a glycosaminoglycan-based hydrogel culture system. This microenvironment model can routinely recreate breast and prostate tumour vascularisation. The multiple cell types cultured within this model were less sensitive to chemotherapy when compared with two dimensional (2D) cultures, and displayed comparative tumour regression to that displayed in vivo. These features highlight the use of our in vitro culture model as a complementary testing platform in conjunction with animal models, addressing key reduction and replacement goals of the future. We anticipate that this biomimetic model will provide a platform for the in-depth analysis of cancer development and the discovery of novel therapeutic targets.

Published
2015

27. A star-PEG–heparin hydrogel platform to aid cell replacement therapies for neurodegenerative diseases

Author

Freudenberg, Uwe, primary, Hermann, Andreas, additional, Welzel, Petra B., additional, Stirl, Katja, additional, Schwarz, Sigrid C., additional, Grimmer, Milauscha, additional, Zieris, Andrea, additional, Panyanuwat, Woranan, additional, Zschoche, Stefan, additional, Meinhold, Dorit, additional, Storch, Alexander, additional, and Werner, Carsten, additional

Published
2009
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30. Electrostatic Interactions Modulate the Conformation of Collagen I

Author

Freudenberg, Uwe, primary, Behrens, Sven H., additional, Welzel, Petra B., additional, Müller, Martin, additional, Grimmer, Milauscha, additional, Salchert, Katrin, additional, Taeger, Tilman, additional, Schmidt, Kati, additional, Pompe, Wolfgang, additional, and Werner, Carsten, additional

Published
2007
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