1. Quantitative method for conjugated metabolites of bisphenol A and bisphenol S determination in food of animal origin by Ultra High Performance Liquid Chromatography–Tandem Mass Spectrometry
- Author
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Catherine Viguié, Bruno Veyrand, Emmanuelle Bichon, Yoann Deceuninck, B. Le Bizec, Philippe Marchand, T. Gény, Flore C. Grandin, Laboratoire d'étude des Résidus et Contaminants dans les Aliments (LABERCA), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), ToxAlim (ToxAlim), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole d'Ingénieurs de Purpan (INPT - EI Purpan), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA), Exposition, Perturbation Endocrino-métabolique et Reproduction (ToxAlim-EXPER), and Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3)
- Subjects
endocrine system ,Bisphenol A ,Bisphenol S ,[SDV]Life Sciences [q-bio] ,UHPLC–MS/MS ,Food Contamination ,Sulfuric Acid Esters ,Isotope dilution ,010402 general chemistry ,01 natural sciences ,Biochemistry ,Analytical Chemistry ,chemistry.chemical_compound ,Glucuronides ,Phenols ,Limit of Detection ,Pregnancy ,Tandem Mass Spectrometry ,Liquid chromatography–mass spectrometry ,Animals ,Sulfones ,Solid phase extraction ,Benzhydryl Compounds ,Chromatography, High Pressure Liquid ,Detection limit ,Foodstuffs ,Sheep ,Chromatography ,urogenital system ,Solid Phase Extraction ,010401 analytical chemistry ,Organic Chemistry ,Extraction (chemistry) ,General Medicine ,Contamination ,0104 chemical sciences ,chemistry ,Female ,hormones, hormone substitutes, and hormone antagonists ,Conjugated metabolites ,Food Analysis - Abstract
International audience; With the objectives of both generating bisphenols (BPs) conjugates occurrence data in food from animal origin but also investigating the origin of associated contamination, the present study deals with the development of an efficient analytical method aiming at monitoring both BPA and BPS conjugated metabolites in food from animal origin. The objective of such monitoring is to determine the origin of BPs contamination (FCM or animal contamination). The targeted compounds were BPA-monoglucuronide (BPA-1G), BPA-diglucuronide (BPA-2G), BPA-monosulfate (BPA-1S), BPA-disulfate (BPA-2S) and BPS-monoglucuronide (BPS-1G). The developed standard operating procedure includes a preliminary solid-liquid extraction step followed by two successive solid phase extraction (SPE) stages, using successively a non-polar phase and a strong cation exchange polymer. Quantification was achieved according to both the isotopic dilution and surrogated quantification methods, using 13C-BPA-1G and BPA-d6-1S as internal standards. Linearity was validated (R2 > 0.99) for each molecule within the concentration range [0-10] μg kg-1. Detection limits ranged from 0.02 μg kg-1 (BPA-1G in muscle, BPA-1S and BPA-2G in liver) to 0.50 μg kg-1 (BPA-2S in muscle). The strategy was then proven on liver samples collected from pregnant ewes subcutaneously exposed to BPA during 105 days, at 50 μg kg-1 per day. BPA-1G, BPA-2G and BPA-1S were detected and quantified at a concentration of 3.81 μg kg-1, 0.80 μg kg-1 and 0.09 μg kg-1, respectively. The analytical method was finally implemented on fifty unpacked food samples from animal origin in which significant free BPA concentrations were previously measured. Since no metabolites of BPA could be measured (
- Published
- 2019