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21 results on '"Ferry, B"'

9. Isolation of cell island cytotrophoblast from first trimester human placenta, and comparison of antigen expression on villus and extravillus cytotrophoblast

Author

Christopher W.G. Redman, Linda Clover, Ferry B, Ian L. Sargent, P.M. Starkey, and S.C. Shorter

Subjects
Cytotrophoblast, Stromal cell, medicine.drug_class, Decidua, Obstetrics and Gynecology, Trophoblast, Biology, Monoclonal antibody, Molecular biology, medicine.anatomical_structure, Syncytiotrophoblast, Reproductive Medicine, Antigen, embryonic structures, Immunology, medicine, Decidual cells, reproductive and urinary physiology, Developmental Biology
Abstract

Summary Antigen expression of the various trophoblast populations in human first trimester placenta was tested using a panel of anti-trophoblast monoclonal antibodies on frozen tissue sections. A method was developed for the isolation of extravillus cytotrophoblast from cell islands, up to 99% pure. These cells were used as an immunogen in mice and the resulting monoclonal antibody, designated NDOG5, was characterized. In first trimester tissue, NDOG5 binds to the cell islands and to the interstitial and endovascular cytotrophoblast of the maternal decidua. At term, the antibody also labels a subset of cytotrophoblast in the amniochorion. It does not react with either syncytiotrophoblast or villus cytotrophoblast. The antigen detected by NDOG5 is specific for extravillus cytotrophoblast, and is not present on amniotic epithelium, decidual cells, or villus stromal cells. NDOG5 does not bind to any normal adult human tissues tested and is not present on breast or cervical carcinoma cells. In relation to other anti-trophoblast antibodies, NDOG5 defines a unique subset of extravillus cytotrophoblast.

Published
1994

10. Isolation and characterisation of a subpopulation of human chorionic cytotrophoblast using a monoclonal anti-trophoblast antibody (NDOG2) in flow cytometry

Author

Ferry B, P.M. Starkey, Ian L. Sargent, Christopher W.G. Redman, and J.J. Caulfield

Subjects
medicine.drug_class, Immunology, Cell Separation, Biology, Monoclonal antibody, Flow cytometry, Antigen, Pregnancy, medicine, Humans, Immunology and Allergy, Cytotrophoblast, medicine.diagnostic_test, Immunoperoxidase, Antibodies, Monoclonal, Obstetrics and Gynecology, Trophoblast, Chorion, Flow Cytometry, Molecular biology, Trophoblasts, medicine.anatomical_structure, Reproductive Medicine, Antigens, Surface, Monoclonal, biology.protein, Female, Antibody
Abstract

Human cytotrophoblast cells, isolated from term amniochorion by enzymic digestion and Percoll gradient centrifugation, were characterised by flow cytometry. A panel of 12 anti-trophoblast monoclonal antibodies was screened for labelling of these cells in flow cytometry and the results compared with immunoperoxidase labelling of cytospin preparations and tissue sections. All 12 antibodies were positive for trophoblast on tissue sections, 11/12 were positive on cytospins but only two (NDOG2 and GB25) gave consistent results in flow cytometry. Two-colour labelling with NDOG2 and W6/32, an antibody to HLA-A, -B, -C, demonstrated that 88% of the NDOG2-positive cells also express Class I major histocompatibility complex (MHC) antigens. The NDOG2-positive cytotrophoblast subpopulation was isolated by flow cytometry in sufficient purity (greater than 95%) and yield (3.1 x 10(6)) for use in functional studies in vitro.

Published
1992

21. Phenotype and functions of decidual LGL

Author

Ferry B, Christopher W.G. Redman, P.M. Starkey, and Ian L. Sargent

Subjects
Reproductive Medicine, Immunology, Obstetrics and Gynecology, Immunology and Allergy, Biology, Phenotype, Cell biology
Published
1989

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