1. Comparison of soxhlet, ultrasound-assisted and pressurized liquid extraction of terpenes, fatty acids and Vitamin E from Piper gaudichaudianum Kunth
- Author
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Rosângela Assis Jacques, Elina Bastos Caramão, Migdalia Miranda Martínez, Maria Ines Soares Melecchi, Jenifer Saffi, Fernanda Contieri Abad, Valéria Flores Péres, and Eniz Conceição Oliveira
- Subjects
Chromatography ,Terpenes ,Chemistry ,Vitamin E ,medicine.medical_treatment ,Fatty Acids ,Organic Chemistry ,Extraction (chemistry) ,General Medicine ,Chemical Fractionation ,Biochemistry ,Gas Chromatography-Mass Spectrometry ,Terpenoid ,Analytical Chemistry ,Solvent ,Terpene ,Pressure ,medicine ,Ultrasonics ,Sample preparation ,Gas chromatography ,Gas chromatography–mass spectrometry ,Piper - Abstract
This paper describes a comparative study of extraction methods of terpenes (terpenic alcohols and phytosterols), fatty acids and Vitamin E from leaves of Piper gaudichaudianum Kunth. The analysis of extracts was done by gas chromatography with mass spectrometric detection. The identification and quantification was made by co-injections of the extract with certified standards. The use of pressurized liquid extraction (PLE; Dionex trade name: ASE, for accelerated solvent extraction) decrease significantly the total time of extraction, the amount of solvent and the manipulation of sample and solvents in comparison with soxhlet (SE) and ultrasound-assisted (USE). In addition, PLE was more effective for the extractions of terpenes (terpenic alcohols and phytosterols), fatty acids and Vitamin E.
- Published
- 2006
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