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The individuation of parameters that characterize decorations in “glass paste” of Etruscan jewels in Meridional Etruria area, by the use of scientific methodologies, has the aim to collocate this typology of manufacts in a historical and geographical context and to improve the knowledge of the technology connected with their realization. The term “glass paste” is widely used in humanistic literature to describe different materials, all based on a “glass like” composition, but its definition is lacking or confusing if the term is not referred to a scientific meaning. The glass analysis is difficult owing to the complexity of the elements that compose the various classes of vitreous materials. For this reason the interpretation of the composition table containing the values of the numerous glass elements, induces the use of statistical methods for the elaboration of the results. The multivariate analysis can be a valid tool for an immediate lecture of the common characteristics of the different typologies of vitreous materials. In this work, the application of the Principal Component Analysis (PCA), has been applied for the elaboration of chemical composition data obtained by electronic microanalysis (SEM-EDS) on findings coming from Etruscan archaeological sites of Nepi and Cerveteri, and on pearls of a necklace from Castellani's Collection. Besides, an attempt has been made to compare the chemical elements found in the vitreous materials of the jewelry with the elements of sands from probable sites along the rivers present in the Etruscan sites by following the indications reported by historical fonts. The obtained results furnished information in various directions, either for the characterization of each historical sample analyzed or the validation of the applied statistical methodology for the composition data elaboration, besides it has been possible to contribute in the knowledge of the possible existence of glass production sites in the Meridional Etruria, more specifically for the realization of decoration in glass paste.