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Your search keyword '"De Matteis, Valeria"' showing total 13 results
Start Over Author "De Matteis, Valeria" Publisher elsevier bv
13 results on '"De Matteis, Valeria"'

4. Aloe Vera Silver Nanoparticles addition in Chitosan Films: Improvement of Physicochemical Properties for Eco-friendly Food Packaging Material

Author

De Matteis, Valeria, primary, Cascione, Mariafrancesca, additional, Costa, Daniele, additional, Martano, Simona, additional, Manno, Daniela, additional, Cannavale, Alessandro, additional, Mazzotta, Stefano, additional, Paladini, Fabio, additional, Martino, Maurizio, additional, and Rinaldi, Rosaria, additional

Published
2023
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13. Morphomechanical and structural changes induced by ROCK inhibitor in breast cancer cells

Author

Chiara Cristina Toma, Stefano Leporatti, Mariafrancesca Cascione, Valeria De Matteis, Rosaria Rinaldi, Paolo Pellegrino, Cascione, Mariafrancesca, De Matteis, Valeria, Toma, Chiara Cristina, Pellegrino, Paolo, Leporatti, Stefano, and Rinaldi, Rosaria

Subjects
Amide, 0301 basic medicine, Pyridines, Pyridine, Cell, Breast Neoplasms, Cancer cell, Biology, Microscopy, Atomic Force, 03 medical and health sciences, chemistry.chemical_compound, Y-27632, MCF-7 Cell, 0302 clinical medicine, Cell Movement, Elastic Modulus, rho-Associated Kinase, ROCK, Cell Adhesion, Tumor Cells, Cultured, medicine, Humans, Cytoskeleton, Protein kinase A, Cell Shape, Rho-associated protein kinase, rho-Associated Kinases, Elastic Modulu, Cell migration, Cell Biology, Amides, Elasticity, Cell biology, Biomechanical propertie, 030104 developmental biology, medicine.anatomical_structure, chemistry, Apoptosis, 030220 oncology & carcinogenesis, MCF-7 Cells, Female, Stress, Mechanical, AFM, Breast Neoplasm, Human
Abstract

The EMT phenomenon is based on tumour progression. The cells lose their physiologic phenotype and assumed a mesenchymal phenotype characterized by an increased migratory capacity, invasiveness and high resistance to apoptosis. In this process, RHO family regulates the activation or suppression of ROCK (Rho-associated coiled-coil containing protein kinase) which in turn regulates the cytoskeleton dynamics. However, while the biochemical mechanisms are widely investigated, a comprehensive and careful estimation of biomechanical changes has not been extensively addressed. In this work, we used a strong ROCK inhibitor, Y-27632, to evaluate the effects of inhibition on living breast cancer epithelial cells by a biomechanical approach. Atomic Force Microscopy (AFM) was used to estimate changes of cellular elasticity, quantified by Young's modulus parameter. The morphometric alterations were analyzed by AFM topographies and Confocal Laser Scanning Microscopy (CLSM). Our study revealed a significant modification in the Young's modulus after treatment, especially as regards cytoskeletal region. Our evidences suggest that the use of Y-27632 enhanced the cell rigidity, preventing cell migration and arrested the metastasization process representing a potential powerful factor for cancer treatment.

Published
2017

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