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Your search keyword '"DE BORTOLI, M"' showing total 26 results
Start Over Author "DE BORTOLI, M" Publisher elsevier bv
26 results on '"DE BORTOLI, M"'

2. 073_16729-B2 Co-Inheritance of Mutations Associated With Arrhythmogenic Cardiomyopathy and Hypertrophic Cardiomyopathy

Author

De Bortoli, M., primary, Calore, C., additional, Lorenzon, A., additional, Calore, M., additional, Poloni, G., additional, Mazzotti, E., additional, Rigato, I., additional, Marra, M. Perazzolo, additional, Melacini, P., additional, Iliceto, S., additional, Thiene, G., additional, Basso, C., additional, Daliento, L., additional, Corrado, D., additional, Bauce, B., additional, and Rampazzo, A., additional

Published
2017
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4. Hepcidin and ferritin blood level as noninvasive tools for predicting breast cancer

Author

Orlandi, R., primary, De Bortoli, M., additional, Ciniselli, C.M., additional, Vaghi, E., additional, Caccia, D., additional, Garrisi, V., additional, Pizzamiglio, S., additional, Veneroni, S., additional, Bonini, C., additional, Agresti, R., additional, Daidone, M.G., additional, Morelli, D., additional, Camaschella, C., additional, Verderio, P., additional, and Bongarzone, I., additional

Published
2014
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5. Proteomic profile in familial breast cancer patients

Author

Garrisi, V.M., primary, Tommasi, S., additional, Facchiano, A., additional, Bongarzone, I., additional, De Bortoli, M., additional, Cremona, M., additional, Cafagna, V., additional, Abbate, I., additional, Tufaro, A., additional, Quaranta, M., additional, and Paradiso, A., additional

Published
2013
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25. Hepcidin and ferritin blood level as noninvasive tools for predicting breast cancer

Author

Italia Bongarzone, Roberto Agresti, Rosaria Orlandi, Chiara Maura Ciniselli, Dario Caccia, Daniele Morelli, Vito Michele Garrisi, Chiara Bonini, Maria Grazia Daidone, Paolo Verderio, M. De Bortoli, C. Camaschella, Elena Vaghi, Silvia Veneroni, Sara Pizzamiglio, Orlandi, R, DE BORTOLI, M, Ciniselli, Cm, Vaghi, E, Caccia, D, Garrisi, V, Pizzamiglio, S, Veneroni, S, Bonini, C, Agresti, R, Daidone, Mg, Morelli, D, Camaschella, Clara, Verderio, P, and Bongarzone, I.

Subjects
Adult, Oncology, medicine.medical_specialty, Adolescent, Breast Neoplasms, Young Adult, Breast cancer, Hepcidins, Hepcidin, Internal medicine, Biomarkers, Tumor, medicine, Humans, Early Detection of Cancer, Aged, chemistry.chemical_classification, Receiver operating characteristic, biology, business.industry, Case-control study, Hematology, Middle Aged, medicine.disease, Confidence interval, Ferritin, Ferritin light chain, ROC Curve, chemistry, Transferrin, Case-Control Studies, Ferritins, biology.protein, Female, business
Abstract

Background Currently used CA15–3 and CEA have found their clinical application particularly in the follow-up of patients with advanced disease. Novel biomarkers are urgent, especially for improving early diagnosis as well as for discriminating between benign and malignant disease. Patients and methods In the present study, we used a proteomic approach based on surface-enhanced laser desorption/ionization–time of flight–mass spectrometry screening with the aim of identifying differentially expressed 2–30 kDa proteins in plasma of patients with malignant (65 cases) and benign (88 cases) breast lesions with respect to 121 healthy controls. Results We found that the most promising SELDI peaks were those corresponding to hepcidin-25 and ferritin light chain. We evaluated the capability of these peaks in predicting malignant and benign breast lesions using the area under the receiver operating characteristic curve (AUC). The results showed a good capacity to predict malignant breast lesions for hepcidin-25 [AUC: 0.82; 95% confidence interval (CI) 0.75–0.90] and ferritin light chain (AUC: 0.86; 95% CI 0.79–0.92). Conversely, a weak and satisfactory capability to predict benign breast lesion was observed for hepcidin-25 (AUC: 0.63; 95% CI 0.41–0.85) and ferritin light chain (AUC: 0.73; 95% CI 0.49–0.97). A significant association between HER2 status and hepcidin-25 was observed and the distribution of transferrin and ferritin were found significantly different in patients with breast cancer when compared with that of controls. Conclusions This study provides evidence that hepcidin and ferritin light chain level in plasma may be of clinical usefulness to predict malignant and benign disease with respect to healthy controls.

Published
2014

26. Quantitative expression profiling of highly degraded RNA from formalin-fixed, paraffin-embedded breast tumor biopsies by oligonucleotide microarrays

Author

Alessandra Vigilante, Margherita Mutarelli, Ornella Paris, Maria Ravo, Olì M. V. Grober, Alessandro Weisz, Roberta Tarallo, E. Nola, Luigi Cicatiello, Daniela Cimino, Lorenzo Ferraro, Michele De Bortoli, Ravo, M., Mutarelli, M., Ferraro, L., Grober, O. M. V., Paris, O., Tarallo, R., Vigilante, A., Cimino, D., DE BORTOLI, M., Nola, Ernesto, Cicatiello, L., and Weisz, A.

Subjects
Microarray, breast cancer, formalin-fixed tissues, microarrays, Gene Expression, Biopsy, Breast Neoplasms, Biology, Pathology and Forensic Medicine, Cell Line, Tumor, Formaldehyde, Gene expression, medicine, Humans, RNA, Neoplasm, Molecular Biology, Oligonucleotide Array Sequence Analysis, Paraffin Embedding, medicine.diagnostic_test, Microarray analysis techniques, Gene Expression Profiling, Carcinoma, Ductal, Breast, Reproducibility of Results, RNA, Cell Biology, Molecular biology, In vitro, Gene expression profiling, expression profiling, Female, DNA microarray, microarray
Abstract

Microarray-based gene expression profiling is well suited for parallel quantitative analysis of large numbers of RNAs, but its application to cancer biopsies, formalin-fixed paraffin-embedded (FFPE) archived tissues in particular, is limited by the poor quality of the RNA recovered. This represents a serious drawback, since FFPE tumor tissue banks are available with clinical and prognostic annotations, which could be exploited for molecular profiling studies, provided that reliable analytical technologies are found. We applied and evaluated here a microarray-based cDNA-mediated annealing, selection, extension and ligation (DASL) assay for analysis of 502 mRNAs in highly degraded total RNA extracted from cultured cells or FFPE breast cancer biopsies. The study included quantitative and qualitative comparison of data obtained by analysis of the same RNAs with genome-wide oligonucleotide microarrays vs. DASL arrays and, by DASL, before and after extensive in vitro RNA fragmentation. The DASL-based expression profiling assay applied to RNA extracted from MCF-7 cells before or after 24 hours stimulation with a mitogenic dose of 17β-estradiol consistently allowed to detect hormone-induced gene expression changes also following extensive RNA degradation in vitro. Comparable results where obtained with tumor RNA extracted from FFPE breast cancer biopsies (6 to 19 years old). The method proved itself sensitive, reproducible and accurate, when compared with results obtained by microarray analysis of RNA extracted from snap-frozen tissue of the same tumor.

Published
2008

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