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Background: In 2020, cervical cancer was the fourth most common cancer globally, with an incidence of 13·3. In Argentina, 7548 new cases are diagnosed each year, with 3932 women dying from this cause. This study aims to show the clinical implementation of a cervical cancer screening program by concurrent HPV testing and cervical cytology (co-testing), in women aged 30-65 years, at a University Hospital setting; and to analyze the five-year cumulative incidence rate of cervical cancer. Methods: Between July 2013 and June 2018, cervical samples were collected from women between the ages of 30 to 65 for cervical cancer screening via co-testing (HC2/PAP). Descriptive statistical analysis, multinomial logistic regression and classification metrics were applied over the data. Findings: A total of 2273 women were included. 91·11% of the participants tested double negative, 2·55% double positive, 5·90% HC2-positive/PAP-negative, and 0·44% HC2-negative/PAP-positive. Of the 171 women with follow-up who tested HC2-positive, 39·77% cleared the viral infection, 37·43% showed viral persistence, and 22·81% were treated after the detection via colposcopy/biopsy of histological HSIL. Interpretation: The prevalence of hrHPV in this population was 0·08, with HSIL histology detection rates of 17·32 per 1000 screened women. Cytology could be considered a prognostic factor in women with an HC2-positive test, an association which has not yet been described. This evidence suggests that the introduction of co-testing could diminish the burden of cervical cancer in low- and middle-income countries, acting as a tool against inequity in healthcare. Funding: The implementation of this program was possible thanks to the inclusion of cotesting into CEMIC’s private health insurance coverage. Declaration of Interest: VD has received personal fees (as a consultant and/or speaker bureau) from Thermo Fisher, BMS, Novartis, Amgen, and Roche; and their researches are sponsored by BMS, Novartis, Amgen, AstraZeneca, and Roche, unrelated to the current work. FvP has been sponsored by Roche and Megablabs (academic grant/attending meetings), unrelated to the current work. VF has been sponsored by Roche (academic grant), unrelated to the current work. AA has been sponsored by AMGEN (academic grant), unrelated to the current work. FM has been sponsored by Tecnolab and Roche (academic grant/attending meetings), unrelated to the current work. The other authors declare no conflict of interest. Ethical Approval: This protocol was reviewed and approved by the local Institutional Review Board (IRB).

Objective ST-segment elevation myocardial infarction (STEMI) has an important economic burden that poised the urgent need to evaluate its catastrophic medical expense. This study evaluates the first 5 years of the national health initiative called Popular Insurance (PI) at the National Institute of Cardiology in Mexico. Study design Retrospective data analysis. Methods STEMI patients with ( n = 317 ) and without ( n = 260 ) PI were selected. Analysed variables included socio-economical context, management care, cost evaluation and three outcomes (mortality, hospital readmission and therapeutic adherence). Descriptive statistical analyses, Kaplan–Meier survival and Support Vector Machine models were used accordingly. Results Treatment costs were higher for PI-covered individuals ( P = 0.022 ) and only 1.89 % of them remained in debt, in contrast to 16.15 % of those without PI. Statistically significant differences were found in relation to days in hospital wards ( P 0.001 ) , imaging studies ( P 0.001 ) and surgical materials ( P = 0.04 ) . Survival analysis ( P = 0.44 ) and therapeutic adherence ( P = 0.38 ) showed no differences. Hospital readmission was predicted with an 81.97% accuracy. The most important predictive variables included were stent type, number of days at the coronary care unit and hospital wards. Conclusions The PI has proven to be a successful program where no differences were found in terms of health care and survival, whereas it provides timely financial support for families facing catastrophic health challenging events.

Whether the treatment of Parkinson's disease has to be initiated with levodopa or a D2 agonist like pramipexole remains debatable. Levodopa is more potent against symptoms than D2 agonists, but D2 agonists are less prone to induce motor complications and may have neuroprotective effects. Although regulation of plastic changes in striatal circuits may be the key to their different therapeutic potential, the gene expression patterns induced by de novo treatments with levodopa or D2 agonists are currently unknown. By studying the whole striatal transcriptome in a rodent model of early stage Parkinson's disease, we have identified the gene expression patterns underlying therapeutically comparable chronic treatments with levodopa or pramipexole. Despite the overall relatively small size of mRNA expression changes at the level of individual transcripts, our data show a robust and complete segregation of the transcript expression patterns induced by both treatments. Moreover, transcripts related to oxidative metabolism and mitochondrial function were enriched in levodopa-treated compared to vehicle-treated and pramipexole-treated animals, whereas transcripts related to olfactory transduction pathways were enriched in both treatment groups compared to vehicle-treated animals. Thus, our data reveal the plasticity of genetic striatal networks possibly contributing to the therapeutic effects of the most common initial treatments for Parkinson's disease, suggesting a role for oxidative stress in the long term complications induced by levodopa and identifying previously overlooked signaling cascades as potentially new therapeutic targets. Fil: Taravini, Irene Rita Eloisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; Argentina Fil: Larramendy, Celia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; Argentina Fil: Gomez, Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; Argentina Fil: Saborido, Mariano Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; Argentina Fil: Spaans, Floor. University of Groningen; Países Bajos Fil: Fresno Rodríguez, Cristóbal. Area de Cs. Agrarias, Ingeniería, Cs. Biológicas y de la Salud de la Universidad Catolica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: González, Germán A.. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Area de Cs. Agrarias, Ingeniería, Cs. Biológicas y de la Salud de la Universidad Catolica de Córdoba; Argentina Fil: Fernandez, Elmer Andres. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Area de Cs. Agrarias, Ingeniería, Cs. Biológicas y de la Salud de la Universidad Catolica de Córdoba; Argentina Fil: Murer, Mario Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina Fil: Gershanik, Oscar Samuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina

Adult male Llamas (Lama glama) (n = 6) were collected using a receptive female and a thermo-electric artificial vagina assembled with a polyethylene collection bag with the following objectives: (a) to develop a reliable and repeatable semen collection technique for Llama, (b) validate semen evaluation tests for Llama, and (c) to determine the effect of collection frequency and season on Llama semen characteristics. First, the semen and sperm variables were recorded and validated through their own repeatability. Semen collection intervals tested were: 1X/week for three weeks and 3X/week for another three weeks, the second collection period occurring after two weeks of sexual rest. The collection frequency of 3X/week significantly reduced (P

The aim of this study was to analyse the biological response to different recombinant human FSH (rhFSH)glycosylation variants on the endocrine activity and gene expression at whole-genome scale in human granulosa-like tumor cell line, KGN. The effects of differences in rhFSH sialylation and oligosaccharide complexity were determined on steroid hormone and inhibin production. A microarray approach was used to explore gene expression patterns induced by rhFSH glycosylation variants. Set enrichment analysis revealed that hormone sialylation and oligosaccharide complexity in rhFSH differentially affected the expression of genes involved in essential biological processes and molecular functions of KGN cells. The relevance of rhFSH oligosaccharide structure on teroidogenesis was confirmed assessing gene expression by real time-PCR. The results demonstrate that FSH oligosaccharide structure affects expression of genes encoding proteins, growth factors and hormones essential for granulosa cells function. Fil: Loreti, Nazareth. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina Fil: Fresno, Cristobal. Universidad Católica de Córdoba. Facultad de Ingeniería. Biomedical Data Mining Group; Argentina Fil: Barrera, David. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán. Departamento de Biología de la Reproducción; México Fil: Andreone, Luz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina Fil: Lira Albarran, Saúl. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán. Departamento de Biología de la Reproducción; México Fil: Fernandez, Elmer Andres. Universidad Católica de Córdoba. Facultad de Ingeniería. Biomedical Data Mining Group; Argentina Fil: Larrea, Fernando. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán. Departamento de Biología de la Reproducción; México Fil: Campo, Stella Maris. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina