1. Programmable protein circuit design
- Author
-
Michael B. Elowitz and Zibo Chen
- Subjects
0303 health sciences ,Orthogonality (programming) ,Interface (Java) ,Circuit design ,Distributed computing ,Cellular functions ,Proteins ,Biology ,Cellular Reprogramming ,Article ,General Biochemistry, Genetics and Molecular Biology ,Cell Physiological Phenomena ,03 medical and health sciences ,Synthetic biology ,0302 clinical medicine ,Composability ,Animals ,Humans ,Synthetic Biology ,Genetic Engineering ,Set (psychology) ,030217 neurology & neurosurgery ,030304 developmental biology ,Electronic circuit - Abstract
A fundamental challenge in synthetic biology is to create molecular circuits that can program complex cellular functions. Because proteins can bind, cleave, and chemically modify one another, and interface directly and rapidly with endogenous pathways, they could extend the capabilities of synthetic circuits beyond what is possible with gene regulation alone. However, the very diversity which makes proteins so powerful also complicates efforts to harness them as well-controlled synthetic circuit components. Recent work has begun to address this challenge, focusing on principles such as orthogonality and composability that permit construction of diverse circuit-level functions from a limited set of engineered protein components. These approaches are now enabling the engineering of circuits that can sense, transmit, and process information, dynamically control cellular behaviors, and enable new therapeutic strategies, establishing a powerful paradigm for programming biology.
- Published
- 2021
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