5 results on '"Cheng-Li Song"'
Search Results
2. Genomic sequencing identifies WNK2 as a driver in hepatocellular carcinoma and a risk factor for early recurrence
- Author
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Jia Fan, Yi-Jie Luo, Cheng-Li Song, Zhi-Qiang Hu, Ya Cao, Ying-Hong Shi, Zheng-Jun Zhou, Xiao-Wu Huang, Chu-Bin Luo, Xin-Rong Yang, Zheng Wang, Jian Zhou, Shao-Lai Zhou, and Hao-Yang Xin
- Subjects
Male ,0301 basic medicine ,Oncology ,China ,medicine.medical_specialty ,Carcinoma, Hepatocellular ,Protein Serine-Threonine Kinases ,Metastasis ,03 medical and health sciences ,symbols.namesake ,Exon ,RUNX1 Translocation Partner 1 Protein ,0302 clinical medicine ,Germline mutation ,Internal medicine ,Exome Sequencing ,Biomarkers, Tumor ,medicine ,Hepatectomy ,Humans ,Genes, Tumor Suppressor ,Genetic Predisposition to Disease ,Risk factor ,beta Catenin ,Sanger sequencing ,Hepatology ,business.industry ,Liver Neoplasms ,RUNX1T1 ,Middle Aged ,medicine.disease ,digestive system diseases ,030104 developmental biology ,medicine.anatomical_structure ,Hepatocellular carcinoma ,Mutation ,symbols ,Female ,030211 gastroenterology & hepatology ,TSC1 ,Neoplasm Recurrence, Local ,business ,Signal Transduction - Abstract
Background & Aims Early recurrence of hepatocellular carcinoma (HCC) after curative resection is common. However, the association between genetic mechanisms and early HCC recurrence, especially in Chinese patients, remains largely unknown. Methods We performed whole-genome sequencing (49 cases), whole-exome sequencing (18 cases), and deep targeted sequencing (115 cases) on 182 primary HCC samples. Focusing on WNK2, we used Sanger sequencing and qPCR to evaluate all the coding exons and copy numbers of that gene in an additional 554 HCC samples. We also explored the functional effect and mechanism of WNK2 on tumor growth and metastasis. Results We identified 5 genes (WNK2, RUNX1T1, CTNNB1, TSC1, and TP53) harboring somatic mutations that correlated with early tumor recurrence after curative resection in 182 primary HCC samples. Focusing on WNK2, the overall somatic mutation and copy number loss occurred in 5.3% (39/736) and 27.2% (200/736), respectively, of the total 736 HCC samples. Both types of variation were associated with lower WNK2 protein levels, higher rates of early tumor recurrence, and shorter overall survival. Biofunctional investigations revealed a tumor-suppressor role of WNK2: its inactivation led to ERK1/2 signaling activation in HCC cells, tumor-associated macrophage infiltration, and tumor growth and metastasis. Conclusions Our results delineate genomic events that characterize Chinese HCCs and identify WNK2 as a driver of early HCC recurrence after curative resection. Lay summary We applied next-generation sequencing and conducted an in-depth genomic analysis of hepatocellular carcinomas from a Chinese patient cohort. The results delineate the genomic events that characterize hepatocellular carcinomas in Chinese patients and identify WNK2 as a driver associated with early tumor recurrence after curative resection. more...
- Published
- 2019
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Catalog
3. Corrigendum to ‘Genomic sequencing identifies WNK2 as a driver in hepatocellular carcinoma and a risk factor for early recurrence’ [J Hepatol 71 (2019) 1152-63]
- Author
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Shao-Lai Zhou, Zheng-Jun Zhou, Zhi-Qiang Hu, Cheng-Li Song, Yi-Jie Luo, Chu-Bin Luo, Hao-Yang Xin, Xin-Rong Yang, Ying-Hong Shi, Zheng Wang, Xiao-Wu Huang, Ya Cao, Jia Fan, and Jian Zhou
- Subjects
Hepatology - Published
- 2022
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4. Mechanical evaluation of three access devices for laparoendoscopic single-site surgery
- Author
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Xiao-feng Xie, Dong-sheng Zhang, Qian-Lin Zou, Cheng-li Song, and Jiangfan Zhu
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Laparoscopic surgery ,medicine.medical_specialty ,Friction ,Computer science ,medicine.medical_treatment ,Task completion ,Sils port ,Weight-Bearing ,Traction ,Abdomen ,Task Performance and Analysis ,medicine ,Humans ,Minimally Invasive Surgical Procedures ,Mechanical Evaluation ,Simulation ,System of measurement ,Endoscopy ,Equipment Design ,Traction (orthopedics) ,Port (computer networking) ,Laparoscopes ,Surgery ,Single site surgery ,Stress, Mechanical - Abstract
Many access devices have been developed for laparoendoscopic single-site surgery (LESS) during recent years. However, investigations are needed to determine which port is most suitable for this relatively new technique. The aim of this study was to evaluate commonly used ports using mechanical approaches in a training simulator. Any port that required less force and shorter surgery times had superior maneuverability.The following three commercially available access devices were evaluated: Multi-ports, TriPort, and single-incision laparoscopic surgery (SILS) Port. A LESS mechanical evaluation platform was developed to investigate the forces that acted on the instruments in the ports while moving along horizontal and vertical axes. In addition, a strain-force measurement system was used to compare the average load on the ports when performing standard maneuvers. Additionally, the task completion time was recorded when the maneuvers in these ports were completed.During the horizontal displacement of the instrument, the traction forces of the Multi-ports were lower than those of the SILS Port, which were lower than those of the TriPort. The average traction forces were significantly different in pairwise multiple comparisons (P0.05). When the instrument was inserted into the ports, the vertical friction forces of the Multi-ports were the lowest and those of the TriPort were the highest. On extraction of the instrument, the friction forces of the Multi-ports remained the lowest, followed by those of the TriPort and SILS Port. There were statistically significant results among all the devices (P0.05). The average load required to perform the task was less for the SILS Port than that for the TriPort (P0.05). Similarly, the average load for the Multi-ports was significantly less than that for the TriPort (P0.001). The participants who used the Multi-ports had significantly faster task times than those who used the SILS Port or TriPort (P0.005).Compared with the TriPort and SILS Port, the Multi-ports was associated with the least average load and the shortest task performance times in a training simulator. This study demonstrates that the Multi-ports may offer superior maneuverability for LESS. more...
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- 2013
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5. Transgenic Rice Plants Harboring Genomic DNA from Zizania latifolia Confer Bacterial Blight Resistance
- Author
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Cheng-li Song, Fu Yaping, Shao-mei Jiang, Jie Chen, Jian-li Wu, and Wei-wei Shen
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Genetics ,Zizania latifolia ,resistance-gene analog ,Sequence analysis ,Oryza sativa ,Plant Science ,Genetically modified crops ,lcsh:Plant culture ,Plant disease resistance ,Biology ,Genetically modified rice ,Transformation (genetics) ,bacterial blight resistance ,Blight ,transformation-competent artificial chromosome library ,lcsh:SB1-1110 ,gene transfer ,Agronomy and Crop Science ,Gene ,Biotechnology - Abstract
Based on the sequence of a resistance gene analog FZ14 derived from Zizania latifolia (Griseb.), a pair of specific PCR primers FZ14P1/FZ14P2 was designed to isolate candidate disease resistance gene. The pooled-PCR approach was adopted using the primer pair to screen a genomic transformation-competent artificial chromosome (TAC) library derived from Z. latifolia. A positive TAC clone (ZR1) was obtained and confirmed by sequence analysis. The results indicated that ZR1 consisted of conserved motifs similar to P-loop (kinase 1a), kinase 2, kinase 3a and GLPL (Gly-Leu-Pro-Leu), suggesting that it could be a portion of NBS-LRR type of resistance gene. Using Agrobacterium-mediated transformation of Nipponbare mature embryo, a total of 48 independent transgenic T0 plants were obtained. Among them, 36 plants were highly resistant to the virulent bacterial blight strain PXO71. The results indicate that ZR1 contains at least one functional bacterial blight resistance gene. more...
- Published
- 2011
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