1. Alterations of oxygen consumption and extracellular acidification rates by glutamine in PBMCs of SLE patients
- Author
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Hui-Ting Lee, Deh-Ming Chang, Chang-Youh Tsai, Yau-Huei Wei, Tsai-Hung Wu, Chyou-Shen Lee, Siao-Cian Pan, and Chen-Sung Lin
- Subjects
Adult ,Male ,0301 basic medicine ,medicine.medical_specialty ,Glutamine ,chemistry.chemical_element ,Mitochondrion ,Peripheral blood mononuclear cell ,Oxygen ,Impaired mitochondrial respiration ,Cohort Studies ,Disease activity ,Plasma ,03 medical and health sciences ,Basal (phylogenetics) ,Oxygen Consumption ,immune system diseases ,Internal medicine ,medicine ,Humans ,Lupus Erythematosus, Systemic ,Molecular Biology ,Aged ,Cell Biology ,Middle Aged ,Mitochondria ,030104 developmental biology ,Endocrinology ,chemistry ,Leukocytes, Mononuclear ,Molecular Medicine ,Female ,Extracellular acidification - Abstract
We evaluated plasma glutamine levels and basal mitochondrial oxygen consumption rate (mOCRB) and basal extracellular acidification rate (ECARB) of peripheral blood mononuclear cells (PBMCs) of systemic lupus erythematous (SLE) patients and healthy controls (HCs). Lower plasma glutamine levels correlated with higher SLE disease activity indexes (p=0.025). Incubated in DMEM containing 100mg/dL glucose, SLE-PBMCs displayed lower mOCRB (p=0.018) but similar ECARB (p=0.467) to those of HC-PBMCs, and their mOCRB got elevated (p
- Published
- 2019