1. Haemolysis of normal and glutathione-deficient sheep erythrocytes by selenite and tellurite
- Author
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Charles Crowley, Elizabeth M. Tucker, and James D. Young
- Subjects
Erythrocytes ,Time Factors ,chemistry.chemical_element ,In Vitro Techniques ,Biology ,Selenious Acid ,Hemolysis ,Biochemistry ,Lesion ,Selenium ,chemistry.chemical_compound ,medicine ,Animals ,Pharmacology ,chemistry.chemical_classification ,Sheep ,Glutathione ,Haemolysis ,Amino acid ,Glucose ,chemistry ,Lytic cycle ,Tellurium ,medicine.symptom ,Intracellular ,Cysteine - Abstract
Both selenite and tellurite caused lysis of normal sheep erythrocytes in vitro. GSH-deficient sheep erythrocytes were considerably more resistant to haemolysis than normal cells. This effect was independent of the biochemical lesion responsible for GSH-deficiency (amino acid transport lesion or γ-glutamyl cysteine synthetase deficiency). These and other observations directly implicate intracellular GSH in the lytic mechanism. Selenite and tellurite-induced haemolysis therefore provides a simple method for detecting GSH-deficient cells. The lytic effect of selenite may explain some of the symptoms associated with selenium poisoning.
- Published
- 1981
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