1. A PCR-based method for detection and quantification of small RNAs
- Author
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Chanjae Park, Wei Yan, Seungil Ro, Kenton M. Sanders, and Jingling Jin
- Subjects
Small RNA ,Molecular Sequence Data ,Biophysics ,Computational biology ,Biology ,Polymerase Chain Reaction ,Biochemistry ,Article ,law.invention ,Mice ,law ,microRNA ,Animals ,Tissue Distribution ,Base sequence ,Tissue distribution ,Small nucleolar RNA ,Molecular Biology ,Polymerase chain reaction ,Cloning ,Genetics ,Base Sequence ,urogenital system ,Gene Expression Profiling ,Cell Biology ,Gene expression profiling ,MicroRNAs ,Organ Specificity - Abstract
Recent cloning efforts have identified hundreds of thousands of small RNAs including micro RNAs (miRNAs), Piwi-interacting RNAs (piRNAs), and small nucleolar RNAs (snoRNAs). These non-coding small RNAs need to be further validated and characterized by detecting and quantifying their expression in different tissues and during different developmental courses. A simple, accurate, and sensitive method for small RNA expression profiling is in high demand. Here, we report such a PCR-based method.
- Published
- 2006
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