1. Azithromycin susceptibility of Neisseria gonorrhoeae in the USA in 2017: a genomic analysis of surveillance data
- Author
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Hillard Weinstock, Sandra Seby, John Leavitt, Chun Wang, Ryan Ruiz, Tamara Baldwin, Chi Hua, Sancta B. St. Cyr, Ellen N. Kersh, Catherine Dominguez, Brian H. Raphael, Jesse C. Thomas, William M. Shafer, Alesia Harvey, Pamela S. O’Brien, Christina Moore, Olusegun O. Soge, Jillian Loomis, Christian Whelen, Jenny Zhang, Ami Patel, Sopheay Hun, Matthew W. Schmerer, Kim M. Gernert, Karen Schlanger, and Cau D. Pham
- Subjects
Microbiology (medical) ,lcsh:R5-920 ,education.field_of_study ,medicine.drug_class ,Antibiotics ,Population ,lcsh:QR1-502 ,Biology ,Azithromycin ,medicine.disease_cause ,Microbiology ,lcsh:Microbiology ,Agar dilution ,Minimum inhibitory concentration ,Infectious Diseases ,Antibiotic resistance ,23S ribosomal RNA ,Virology ,medicine ,Neisseria gonorrhoeae ,lcsh:Medicine (General) ,education ,medicine.drug - Abstract
Summary: Background: The number of cases of gonorrhoea in the USA and worldwide caused by Neisseria gonorrhoeae is increasing (555 608 reported US cases in 2017, and 87 million cases worldwide in 2016). Many countries report declining in vitro susceptibility of azithromycin, which is a concern because azithromycin and ceftriaxone are the recommended dual treatment in many countries. We aimed to identify strain types associated with decreased susceptibility to azithromycin. Methods: We did a genomic analysis of N gonorrhoeae isolates obtained by the US Gonococcal Isolate Surveillance Project. Isolates were whole-genome sequenced based on decreased susceptibility to azithromycin (minimal inhibitory concentration [MIC] ≥2 μg/mL, using agar dilution antibiotic susceptibility testing) and geographical representation. Bioinformatic analyses established genomic diversity, strain population dynamics, and antimicrobial resistance profiles. Findings: 410 isolates were sorted into more than 20 unique phylogenetic clades. One predominant persistent clade (consisting of 97 isolates) included the most isolates with azithromycin MICs of 2 μg/mL or higher (61 of 97 [63%] vs 59 of 311 [19%]; p
- Published
- 2020
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