1. Toward a comprehensive study for multielemental quantitative LA-ICP MS bioimaging in soft tissues
- Author
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Olivia Sgarbura, Brice Bouyssiere, Sandra Mounicou, Sébastien Carrère, Dominique Michau, Carine Arnaudguilhem, Marion Larroque, François Quenet, Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux (IPREM), Université de Pau et des Pays de l'Adour (UPPA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut du Cancer de Montpellier (ICM), Département de chirurgie, CRLCC Val d'Aurelle - Paul Lamarque, Institut de Chimie de la Matière Condensée de Bordeaux (ICMCB), Université de Bordeaux (UB)-Institut Polytechnique de Bordeaux-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and Aquitaine Region and Feder are acknowledged for funding the laser ablation system through the AQUITRACE project (convention number 20131206001–13010973) and the profilometer.
- Subjects
polymer films ,Analytical chemistry ,chemistry.chemical_element ,Metal toxicity ,02 engineering and technology ,01 natural sciences ,Analytical Chemistry ,Limit of Detection ,[CHIM.ANAL]Chemical Sciences/Analytical chemistry ,La icp ms ,Humans ,bioimaging ,Inductively coupled plasma mass spectrometry ,chemistry.chemical_classification ,Aqueous solution ,Lasers ,Spectrum Analysis ,010401 analytical chemistry ,Soluble polymer ,[CHIM.MATE]Chemical Sciences/Material chemistry ,Polymer ,Reference Standards ,021001 nanoscience & nanotechnology ,quantification ,LA-ICP MS ,0104 chemical sciences ,chemistry ,Metals ,multielemental ,Ionization energy ,0210 nano-technology ,Carbon - Abstract
International audience; Quantitative localization of metals in biological tissue sections is critical to obtain insight into metal toxicity mechanisms or their beneficial characteristics. This study presents the development of a quantitative LA-ICP MS bioimaging methodology based on the polymer film strategy and internal standardization. To maximize the number of elements mapped, an aqueous soluble polymer (dextran) was selected. Among the elements studied, the great majority (eight out eleven), i.e., Co, Ni, Cu, Zn, Se, Mo, Cd and Pt, exhibited linear regression after LA-ICP MS analysis of metal-spiked polymer standards. Methodology performances were carefully assessed as a function of the three internal standards (In, Rh and Ir) considered, the analytical operational conditions (ICP power, addition of O 2 to ICP, and laser fluency) and the thickness of the biological tissue section. The results indicated that three groups (Co, Mo; Ni, Cu, Pt; and Zn, Se, Cd) of elements could be distinguished from their analytical response as a function of analytical conditions and the internal standard. These different element behaviors appeared to be mainly First Ionization Potential dependent (FIP). For elements with lower FIP (Co, Ni, Cu, Mo and Pt), differential responses due to carbon load in the ICP MS plasma could be efficiently corrected as a function of analytical conditions. Matrix effects were more pronounced for higher FIP elements (i.e., Zn, Cd and Se), and analysis of
- Published
- 2021
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