1. GDAP1 mutations in Italian axonal Charcot–Marie–Tooth patients: Phenotypic features and clinical course
- Author
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Pezzini, I, Geroldi, A, Capponi, S, Gulli, R, Schenone, A, Grandis, M, Doria Lamba, L, La Piana, C, Cremonte, M, Nolano, M, Mandich, P, Bellone, E., NOLANO, MARIA, PISCIOTTA, CHIARA, MANGANELLI, FIORE, SANTORO, LUCIO, Pezzini, I, Geroldi, A, Capponi, S, Gulli, R, Schenone, A, Grandis, M, Doria Lamba, L, La Piana, C, Cremonte, M, Pisciotta, Chiara, Nolano, M, Manganelli, Fiore, Santoro, Lucio, Mandich, P, Bellone, E., and Nolano, Maria
- Subjects
Adult ,Male ,0301 basic medicine ,Adolescent ,Dominant inheritance ,DNA Mutational Analysis ,Neural Conduction ,MFN2 ,Nerve Tissue Proteins ,GDAP1 ,Charcot–Marie–Tooth disease ,Biology ,medicine.disease_cause ,Young Adult ,03 medical and health sciences ,0302 clinical medicine ,Charcot-Marie-Tooth Disease ,medicine ,Humans ,Autonomic Pathways ,Copy-number variation ,Young adult ,Child ,Muscle, Skeletal ,Gene ,Genetics (clinical) ,Aged ,Aged, 80 and over ,Family Health ,Genetics ,Mutation ,Axonal CMT ,Middle Aged ,Phenotype ,genomic DNA ,030104 developmental biology ,Italy ,Neurology ,Pediatrics, Perinatology and Child Health ,Cohort ,Female ,Neurology (clinical) ,030217 neurology & neurosurgery - Abstract
Mutations in the ganglioside-induced differentiation associated-protein 1 (GDAP1) gene have been associated with both autosomal recessive (AR) and dominant (AD) Charcot-Marie-Tooth (CMT) axonal neuropathy. The relative frequency of heterozygous, dominant mutations in Italian CMT is unknown. We investigated the frequency of dominant mutations in GDAP1 in a cohort of 109 axonal Italian patients by sequencing genomic DNA and search for copy number variations. We also explored correlations with clinical features. All cases had already been tested for variants in common axonal AD genes. Eight patients (7.3%) harbored five already reported heterozygous mutations in GDAP1 (p.Arg120Gly, p.Arg120Trp, p.His123Arg, p.Gln218Glu, p.Arg226Ser). Mutations had different penetrances in the families; the onset of symptoms is in the first decade and progression is slower than usually seen in GDAP1-related AR-CMT. We show that the relative frequency of mutations in GDAP was slightly higher than those observed in MFN2 and MPZ (7.3% vs 6.3% and 5.0%). The relatively milder clinical features and the quite indolent course observed are relevant for prognostic assessment. On the basis of our experience and the data reported here, we suggest GDAP1 as the first gene that should be analysed in Italian patients affected by CMT2.
- Published
- 2016