1. Characterization of novel cyclic lipopeptides produced by Bacillus sp. SY27F
- Author
-
Hong Zhou, Yongqi Tian, Yu He, Bailin Cong, and Huanghao Yang
- Subjects
0106 biological sciences ,chemistry.chemical_classification ,0303 health sciences ,biology ,Stereochemistry ,Chemistry ,Fatty acid ,Bioengineering ,medicine.disease_cause ,biology.organism_classification ,01 natural sciences ,Applied Microbiology and Biotechnology ,Biochemistry ,Corpus albicans ,HeLa ,03 medical and health sciences ,DU145 ,Staphylococcus aureus ,010608 biotechnology ,medicine ,Cytotoxicity ,Antibacterial activity ,Escherichia coli ,030304 developmental biology - Abstract
Three new cyclic lipopeptides (compounds 1–3) were isolated from Bacillus sp. SY27F, which collected from the deep-sea hydrothermal vent in the Indian Ocean. The planar structures of compounds 1–3 were characterized by LC–MS, GC–MS and NMR, whereas their absolute configurations were determined by modified Marfey’s analysis and optical rotation contrast. They shared the same heptapeptide chain (N-L-Gln1-D-Leu2-L-Leu3-L-Val4-L-Asp5-D-Leu6-L-Ile7-C), differing only by the length of the fatty acid chain. Compounds 1–3 displayed significant cytotoxic activity against DU145, MCF-7 and HepG2 cancer cell lines with IC50 values of 96.8–190.7 nM and moderate to weak cytotoxicity against A549, HeLa and HT1080, with IC50 values of 9.3–43.2 μM, respectively. However, none of the three compounds showed any antibacterial activity against Escherichia coli, Staphylococcus aureus and Monilia albicans.
- Published
- 2019