1. Subcellular distribution of phospholipids and of polyprenyl phosphate in Aspergillus niger van tieghem
- Author
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R. Letoublon, B. Mayet, R. Got, Claude Nicolau, and J. Frot-Coutaz
- Subjects
chemistry.chemical_classification ,Chromatography ,biology ,Aspergillus niger ,Biophysics ,Phospholipid ,biology.organism_classification ,Phosphate ,Biochemistry ,chemistry.chemical_compound ,Endocrinology ,Enzyme ,chemistry ,Mannosylation ,Cardiolipin ,Microsome ,Cell fractionation - Abstract
Growth of Aspergillus niger Van Tieghem in presence of 32p1 was followed by cell fractionation; the subcellular fractions were identified using marker enzymes and their phospholipid content was analyzed. Microsomes are characterized by high PC and PS values, whereas the largest amount of cardiolipin is recovered in the mitochondria. Polyprenyl phosphate was found mainly in mitochondria (46%) (microsomal content: 38%) and, more precisely, the highest ratio was in the outer membrane. This situation raises the problem of polyprenyl phosphate transfer from mitochondria to microsomes where the enzyme responsible for polyprenyl phosphate mannosylation is located.
- Published
- 1982
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