1. Predicting the results of competition between two breast cancer lines grown in 3-D spheroid culture
- Author
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Mehdi Damaghi, Robert J. Gillies, Yan Miao, Audrey Freischel, Dorothy Wallace, Joel S. Brown, and Marisabel Rodriguez Messan
- Subjects
0301 basic medicine ,Statistics and Probability ,media_common.quotation_subject ,Population ,Cell Culture Techniques ,Breast Neoplasms ,Cell Communication ,Models, Biological ,General Biochemistry, Genetics and Molecular Biology ,Competition (biology) ,03 medical and health sciences ,0302 clinical medicine ,Breast cancer ,Breast cancer cell line ,Cell Line, Tumor ,medicine ,Humans ,Computer Simulation ,education ,Mda mb 231 ,media_common ,Physics ,education.field_of_study ,Extinction ,General Immunology and Microbiology ,Applied Mathematics ,Spheroid ,General Medicine ,medicine.disease ,030104 developmental biology ,030220 oncology & carcinogenesis ,Modeling and Simulation ,MCF-7 Cells ,Female ,General Agricultural and Biological Sciences ,Biological system - Abstract
This study develops a novel model of a consumer–resource system with mobility included, in order to explain a novel experiment of competition between two breast cancer cell lines grown in 3D in vitro spheroid culture. The model reproduces observed differences in monoculture, such as overshoot phenomena and final size. It also explains both theoretically and through simulation the inevitable triumph of the same cell line in co-culture, independent of initial conditions. The mobility of one cell line (MDA-MB-231) is required to explain both the success and the rapidity with which that species dominates the population and drives the other species (MCF-7) to extinction. It is shown that mobility directly interferes with the other species and that the cost of that mobility is in resource usage rate.
- Published
- 2021
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