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This Article constitutes part of the PhD thesis of Claudia Torno., The replacement of the finite and costly resource fish oil is an important task for aquaculture nutrition. A promising approach could be the use of plant bioactives that may have the potential to influence the metabolism and the synthesis of n-3 long chain polyunsaturated fatty acids, especially EPA (20:5n-3) and DHA (22:6n-3). In this study, the two phytochemicals resveratrol (RV) and genistein (G) were investigated for their effects on fish growth, nutrient utilization and body nutrient composition alongside their effects on whole body fatty acid (FA) composition. In a feeding trial lasting 8 weeks, rainbow trout (initial BW: 81.4±0.5 g) were held in a recirculating aquaculture system and fed six experimental diets with varying fish oil levels as plain variants or supplemented with 0.3% of dry matter (DM) of either RV or G. The six diets were as follows: diet F4 had 4% DM fish oil, diet F0 had 0% DM fish oil, diets F4+RV, F4+G, F0+RV and F0+G were equal to the diets F4 and F0, respectively, and supplemented with the phytochemicals RV and G. The feeding of the F0+RV diet resulted in reduced feed intake, growth rate and slightly reduced whole body lipid levels. At the same time, the amount of polyunsaturated FA and the n-3/n-6 ratio were significantly increased in whole body homogenates of rainbow trout fed diet F0+RV in comparison to the F0 control. The feeding of the F0+G diet led to reduced feed intake, slightly increased protein utilization but did not significantly affect the whole body FA composition. Overall, feeding the fish oil-free diet supplemented with the phytochemicals resulted in more pronounced effects on fish performance and FA composition than the single factors per se (dietary fish oil level or phytochemical). Present data indicate that G might not be of profitable use for trout nutrition. In terms of FA composition, RV could be a potentially useful complement for fish oil. However, the impairment of growth and performance parameters as observed in the present study discourages its use in trout diets., This work was supported by the DFG – Deutsche Forschungsgemeinschaft (grant nos: RI 884/8-1; SCHU 2308/4-1). We are grateful to the Analysis Service Unit facilities of ICTAN for the GC-FID analysis and Spanish Ministerio de Economía, Industria y Competitividad for grant AGL2016-76832-R.

Made available in DSpace on 2022-05-01T11:54:24Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-02-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) This study evaluated the ileal and total tract digestibility of nutrients and energy in soybean extruded at three different conditions, compared to deactivated soybean (DS), in newly weaned piglets. Forty crossbred barrows (28 d old and 7.64 ± 0.88 kg body weight) were allotted to 5 dietary treatments with 8 replications per treatment. The diets consisted of an N-free diet, to determine endogenous losses of amino acids (AA), and 4 diets containing 30% of test ingredients as the only source of protein and AA: soybean extruded at 82 °C (SB82), 122 °C (SB122) and 137 °C (SB137), and DS, which was used as a control. The DS was produced by heating the whole raw soybean, from 63 °C to 107 °C under pressure. Titanium dioxide was added to the diets as an indigestible marker. After a 5 d adaptation period, partial feces collection was performed over 5 d and samples of ileal digesta were collected after piglets euthanasia. The apparent total tract digestibility of dry matter, gross energy (GE), crude protein (CP), neutral detergent fiber and acid detergent fiber, and apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of CP and AA were evaluated. Analysis of variance was performed and the means compared by Tukey test when P < 0.05. The extrusion of soybean at 122 °C and 137 °C was effective in reducing trypsin inhibitors content, and promoted adequate nutrient and energy digestibility of soybean in piglets. These effects could be observed as the AID of Lys and Trp, and SID of Lys of SB122 and SB137 were greater (P < 0.05) than those of SB82 and DS. The AID and SID of CP and remaining AA were similar among SB122, SB137 and DS, but greater (P < 0.05) than those of SB82. In addition, the ATTD of GE of SB122 was 3–4% greater (P < 0.05) than those of DS and SB82, showing an important effect of extrusion on energy use by pigs. Thus, extrusion of soybean at 122 °C is recommended and SB122 is a feed ingredient with greater nutritional value than DS or SB82 for young pigs. University of São Paulo (USP) Luiz de Queiroz College of Agriculture Department of Animal Science São Paulo State University (UNESP) School of Agricultural and Veterinarian Sciences University of São Paulo (USP) Department of Animal Science São Paulo State University (UNESP) School of Agricultural and Veterinarian Sciences CAPES: 1706625 FAPESP: 2018/02343-6 FAPESP: 2019/01849-6 CAPES: 88887.481339/2020-00

Jack bean (Canavalia ensiformis) is one of the underutilized legumes in animal feed production partly because of its high antinutritional factors. This study investigated the nutritional value of C. ensiformis seed subjected to hydrothermal processing in the diet of the African catfish Clarias gariepinus. Five batches of C. ensiformis seeds were hydrothermally processed in boiling water (100 °C) for 0, 10, 20, 30 and 40 min, respectively. Proximate composition of the seed showed no significant effect of hydrothermal processing on protein and fat content of C. ensiformis. However, all essential amino acids were significantly affected. The anti-nutritional factor canavanine was not markedly reduced even at 40 min hydrothermal processing. Fifty fingerlings of C. gariepinus (1.07 ± 0.01 g) were stocked in 15 hapas measuring 1 × 1 × 1m3, labeled in triplicate according to five isonitrogenous diets (35% CP) formulated using the processed C. ensiformis seed at an inclusion level of 27%. The highest body weight gain (2.73 g), specific growth rate (2.26gday−1), feed conversion efficiency (34.11%) and protein efficiency ratio (0.078) were observed at hydrothermal treatment of 30 and 40 min. Hydrothermal processing of C. ensiformis up to 40 min could be exploited in the commercial and on-farm production of catfish diet at 27% level of inclusion. Keywords: Jack bean, Unconventional feed, Hydrothermal, Anti-nutritional factor, African catfish

The effects of air classification and lactic acid bacteria fermentation on the reduction of anti-nutritional factors (vicine and convicine, trypsin inhibitor activity, condensed tannins and phytic acid) and in vitro protein and starch digestibility of faba bean flour were studied. Free amino acid (FAA) profile analysis was also carried out. Air classification allowed the separation of the flour into protein and starch rich fractions, showing different chemical compositions and microstructures. Lactobacillus plantarum growth and acidification in faba bean flour and its fractions were assessed. The anti-nutritional compounds were separated mostly to the fine protein-rich fraction. Fermentation caused the decrease of vicine and convicine contents by more than 91% and significantly reduced trypsin inhibitor activity and condensed tannins (by more than 40% in the protein-rich fraction). No significant (P>0.05) variation was observed for total phenols and phytic acid content. Fermentation increased the amount of FAA, especially of the essential amino acids and γ-aminobutyric acid, enhanced the in vitro protein digestibility and significantly lowered the hydrolysis index. This work showed that the combination of air classification and fermentation improved nutritional functionality of faba bean flour which could be utilized in various food applications.