1. Capillary electrophoresis in biochemical and clinical laboratories
- Author
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Wolfgang Voelter, Rainer Lehmann, Jürgen Schütz, Angelika Weiler, Stanka Stoeva, Ourania E Tsitsiloni, Gerald Grübler, and Gerd Paulus
- Subjects
Desorption ionization ,Chromatography ,Human blood ,Chemistry ,Organic Chemistry ,General Medicine ,Mass spectrometry ,Biochemistry ,Analytical Chemistry ,Electrophoresis ,Matrix-assisted laser desorption/ionization ,Capillary electrophoresis ,Blood serum ,Primary sequence - Abstract
As demonstrated by selected examples from our laboratories, CE is a unique methodology for purity control of synthetic as well as natural tissue-isolated biopolymers, a prerequisite before reliable biotestings should be performed. A combination of rapid matrix-assisted laser desorption ionization mass and CE electrophoretic mobility determinations facilitates primary sequence determinations of enzymatic peptide digest mixtures often making costly Edman degradations unnecessary. The enormous separation efficiency and large variety of different possible separation modes in CE, allow detection of single components in complex mixtures which is demonstrated by the apolipoprotein A-I determination in human blood serum in this communication.
- Published
- 1998