8 results on '"Andrei Stecca Steindorff"'
Search Results
2. Analysis of Trichoderma harzianum TR 274 secretome to assign candidate proteins involved in symbiotic interactions with Phaseolus vulgaris
- Author
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Francilene Lopes da Silva, Elaine Nascimento Aquino, Débora Costa da Cunha, Pedro Ricardo Vieira Hamann, Thales Bruno Magalhães, Andrei Stecca Steindorff, Cirano José Ulhoa, and Eliane F. Noronha
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Bioengineering ,Agronomy and Crop Science ,Applied Microbiology and Biotechnology ,Food Science ,Biotechnology - Published
- 2022
3. Trichoderma/pathogen/plant interaction in pre-harvest food security
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Valdirene Neves Monteiro, Roberto Nascimento Silva, Andrei Stecca Steindorff, Eriston Vieira Gomes, Eliane Ferreira Noronha, and Cirano José Ulhoa
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Crops, Agricultural ,PRÉ-COLHEITA ,Biological pest control ,Food Supply ,Crop ,03 medical and health sciences ,Antibiosis ,Genetics ,Ecology, Evolution, Behavior and Systematics ,Plant Diseases ,030304 developmental biology ,Trichoderma ,0303 health sciences ,Food security ,biology ,030306 microbiology ,Abiotic stress ,business.industry ,fungi ,Fungi ,food and beverages ,biology.organism_classification ,Crop protection ,Biotechnology ,Infectious Diseases ,Agriculture ,business - Abstract
Large losses before crop harvesting are caused by plant pathogens, such as viruses, bacteria, oomycetes, fungi, and nematodes. Among these, fungi are the major cause of losses in agriculture worldwide. Plant pathogens are still controlled through application of agrochemicals, causing human disease and impacting environmental and food security. Biological control provides a safe alternative for the control of fungal plant pathogens, because of the ability of biocontrol agents to establish in the ecosystem. Some Trichoderma spp. are considered potential agents in the control of fungal plant diseases. They can interact directly with roots, increasing plant growth, resistance to diseases, and tolerance to abiotic stress. Furthermore, Trichoderma can directly kill fungal plant pathogens by antibiosis, as well as via mycoparasitism strategies. In this review, we will discuss the interactions between Trichoderma/fungal pathogens/plants during the pre-harvest of crops. In addition, we will highlight how these interactions can influence crop production and food security. Finally, we will describe the future of crop production using antimicrobial peptides, plants carrying pathogen-derived resistance, and plantibodies.
- Published
- 2019
4. Trichoderma harzianum transcriptome in response to cadmium exposure
- Author
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Raphaela de Castro Georg, Letícia Harumi Oshiquiri, Andrei Stecca Steindorff, Thuana Marcolino Mota, Cirano José Ulhoa, Jomal Rodrigues Barbosa Filho, Karina Roterdanny Araújo dos Santos, and Sidnei Alves Ferreira Junior
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Genes, Fungal ,chemistry.chemical_element ,Cellular homeostasis ,Fungus ,Carbohydrate metabolism ,Microbiology ,Transcriptome ,03 medical and health sciences ,Gene Expression Regulation, Fungal ,Genetics ,RNA Processing, Post-Transcriptional ,Mycelium ,030304 developmental biology ,0303 health sciences ,Cadmium ,biology ,030306 microbiology ,Trichoderma harzianum ,Metabolism ,biology.organism_classification ,chemistry ,Biochemistry ,Hypocreales ,Spliceosomes ,Carbohydrate Metabolism ,Protein Modification, Translational - Abstract
Cadmium (Cd) is a heavy metal present in the environment mainly as a result of industrial contamination that can cause toxic effects to life. Some microorganisms, as Trichoderma harzianum, a fungus used in biocontrol, are able to survive in polluted environments and act as bioremediators. Aspects about the tolerance to the metal have been widely studied in other fungi although there are a few reports about the response of T. harzianum. In this study, we determined the effects of cadmium over growth of T. harzianum and used RNA-Seq to identify significant genes and processes regulated in the metal presence. Cadmium inhibited the fungus growth proportionally to its concentration although the fungus exhibited tolerance as it continued to grow, even in the highest concentrations used. A total of 3767 (1993 up and 1774 down) and 2986 (1606 up and 1380 down) differentially expressed genes were detected in the mycelium of T. harzianum cultivated in the presence of 1.0 mg mL−1 or 2.0 mg mL−1 of CdCl2, respectively, compared to the absence of the metal. Of these, 2562 were common to both treatments. Biological processes related to cellular homeostasis, transcription initiation, sulfur compound biosynthetic and metabolic processes, RNA processing, protein modification and vesicle-mediated transport were up-regulated. Carbohydrate metabolic processes were down-regulated. Pathway enrichment analysis indicated induction of glutathione and its precursor’s metabolism. Interestingly, it also indicated an intense transcriptional induction, especially by up-regulation of spliceosome components. Carbohydrate metabolism was repressed, especially the mycoparasitism-related genes, suggesting that the mycoparasitic ability of T. harzianum could be affected during cadmium exposure. These results contribute to the advance of the current knowledge about the response of T. harzianum to cadmium exposure and provide significant targets for biotechnological improvement of this fungus as a bioremediator and a biocontrol agent.
- Published
- 2020
5. Biochemical and metabolic profiles of Trichoderma strains isolated from common bean crops in the Brazilian Cerrado, and potential antagonism against Sclerotinia sclerotiorum
- Author
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Andrei Stecca Steindorff, Valdirene Neves Monteiro, Alexandre Siqueira Guedes Coelho, A. M. Geraldine, Renata Silva Brandão, Murillo Lobo Junior, Cirano José Ulhoa, Fabyano Alvares Cardoso Lopes, and Roberto Nascimento Silva
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Molecular Sequence Data ,Biological pest control ,macromolecular substances ,complex mixtures ,Ascomycota ,Antibiosis ,Botany ,Genetics ,DNA, Fungal ,Pathogen ,Ecology, Evolution, Behavior and Systematics ,Phaseolus ,Trichoderma ,chemistry.chemical_classification ,biology ,Sclerotinia sclerotiorum ,technology, industry, and agriculture ,food and beverages ,Trichoderma harzianum ,Biodiversity ,Sequence Analysis, DNA ,biology.organism_classification ,carbohydrates (lipids) ,Infectious Diseases ,Enzyme ,chemistry ,Metabolome ,Antagonism ,Brazil - Abstract
Some species of Trichoderma have successfully been used in the commercial biological control of fungal pathogens, e.g., Sclerotinia sclerotiorum, an economically important pathogen of common beans (Phaseolus vulgaris L.). The objectives of the present study were (1) to provide molecular characterization of Trichoderma strains isolated from the Brazilian Cerrado; (2) to assess the metabolic profile of each strain by means of Biolog FF Microplates; and (3) to evaluate the ability of each strain to antagonize S. sclerotiorum via the production of cell wall-degrading enzymes (CWDEs), volatile antibiotics, and dual-culture tests. Among 21 isolates, we identified 42.86% as Trichoderma asperellum, 33.33% as Trichoderma harzianum, 14.29% as Trichoderma tomentosum, 4.76% as Trichoderma koningiopsis, and 4.76% as Trichoderma erinaceum. Trichoderma asperellum showed the highest CWDE activity. However, no species secreted a specific group of CWDEs. Trichoderma asperellum 364/01, T. asperellum 483/02, and T. asperellum 356/02 exhibited high and medium specific activities for key enzymes in the mycoparasitic process, but a low capacity for antagonism. We observed no significant correlation between CWDE and antagonism, or between metabolic profile and antagonism. The diversity of Trichoderma species, and in particular of T. harzianum, was clearly reflected in their metabolic profiles. Our findings indicate that the selection of Trichoderma candidates for biological control should be based primarily on the environmental fitness of competitive isolates and the target pathogen.
- Published
- 2012
6. Trichoderma harzianum expressed sequence tags for identification of genes with putative roles in mycoparasitism against Fusarium solani
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Tatsuya Nagata, Andrei Stecca Steindorff, Eliane Ferreira Noronha, Cirano José Ulhoa, Roberto Nascimento Silva, and Alexandre Siqueira Guedes Coelho
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Expressed sequence tag ,biology ,cDNA library ,food and beverages ,Trichoderma harzianum ,biology.organism_classification ,Microbiology ,Insect Science ,Root rot ,Phaseolus ,Agronomy and Crop Science ,Gene ,Fusarium solani ,Mycelium - Abstract
The plant pathogen Fusarium solani causes a disease root rot of common bean (Phaseolus vulgaris) resulting in great losses of yield in irrigated areas of the Southeast and Midwest regions of Brazil. Species of the genus Trichoderma have been used in the biological control of this pathogen as an alternative to chemical control. To gain new insights into the biocontrol mechanism used by Trichoderma harzianum against the phytopathogenic fungus, Fusarium solani, we performed a transcriptome analysis using expressed sequence tags (ESTs) and quantitative real-time PCR (RT-qPCR) approaches. A cDNA library from T. harzianum mycelium (isolate ALL42) grown on cell walls of F. solani (CWFS) was constructed and analyzed. A total of 2927 high quality sequences were selected from 3845 and 37.7% were identified as unique genes. The Gene Ontology analysis revealed that the majority of the annotated genes are involved in metabolic processes (80.9%), followed by cellular process (73.7%). We tested twenty genes that encode proteins with potential role in biological control. RT-qPCR analysis showed that none of these genes were expressed when T. harzianum was challenged with itself. These genes showed different patterns of expression during in vitro interaction between T. harzianum and F. solani.
- Published
- 2012
7. Biochemical characterization of a 27kDa 1,3-β-d-glucanase from Trichoderma asperellum induced by cell wall of Rhizoctonia solani
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Marcelo Henrique Soller Ramada, Andrei Stecca Steindorff, Saulo José Linhares de Siqueira, Cirano José Ulhoa, and Raquel da Silva Aires
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chemistry.chemical_classification ,Polymers and Plastics ,biology ,Characterization ,Organic Chemistry ,Ion chromatography ,Size-exclusion chromatography ,1,3-β-d-glucanase ,Glucanase ,biology.organism_classification ,Trichoderma asperellum ,Aspergillus fumigatus ,Rhizoctonia solani ,Cell wall ,Laminarin ,chemistry.chemical_compound ,Enzyme ,Biochemistry ,chemistry ,Materials Chemistry ,Purification - Abstract
Trichoderma asperellum produces two extracellular 1,3-β- d -glucanase upon induction with cell walls from Rhizoctonia solani. A minor 1,3-β- d -glucanase was purified to homogeneity by ion exchange chromatography on Q-Sepharose and gel filtration on Sephacryl S-100. A typical procedure provided 13.8-fold purification with 70% yield. SDS–PAGE of the purified enzyme showed a single protein band of molecular weight 27 kDa. The enzyme exhibited optimum catalytic activity at pH 3.6 and 45 °C. It was thermostable at 40 °C, and retained 75% activity after 60 min at 45 °C. The Km and Vmax values for 1,3-β- d -glucanase, using laminarin as substrate, were 0.323 mg ml−1 and 0.315 U min−1, respectively. The enzyme was strongly inhibited by Hg2+ and SDS. The enzyme was only active toward glucans containing β-1,3-linkages. Peptide sequences showed similarity with two endo-1,3(4)-β- d -glucanases from Aspergillus fumigatus Af293when compared against GenBank non-redundant database.
- Published
- 2012
8. Expression analysis of the exo-β-1,3-glucanase from the mycoparasitic fungus Trichoderma asperellum
- Author
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Silvana P. da Silva, Roberto Nascimento Silva, César Marcos Marcello, Cirano José Ulhoa, Luiz Artur Mendes Bataus, and Andrei Stecca Steindorff
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Trichoderma ,Regulation of gene expression ,Fungal protein ,biology ,food and beverages ,Glucan 1,3-beta-Glucosidase ,Glucanase ,biology.organism_classification ,Microbiology ,Gene Expression Regulation, Enzymologic ,Enzyme assay ,Fungal Proteins ,Rhizoctonia solani ,Gene Expression Regulation, Fungal ,Gene expression ,Extracellular ,biology.protein - Abstract
The regulation of the gene encoding the extracellular exo-beta-1,3-glucanase (tag83) produced by the mycoparasite Trichoderma asperellum was studied. Enzyme activity was detected in all carbon sources, but the highest levels were found when starch and purified cell walls from Rhizoctonia solani were used. These results are supported by the appearance of one strong band with enzyme activity in non-denaturing PAGE. Experiments using RT-PCR showed that exo-beta-1,3-glucanase induction in T. asperellum occurred at the transcriptional level. We used RT-PCR and real-time PCR analysis to examine the expression of tag83 gene during in vivo assay of T. asperellum against R. solani. We showed that the expression of tag83 is significantly increased by the presence of R. solani.
- Published
- 2010
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