1. Methodological aspects of the molecular and histological study of prostate cancer: Focus on PTEN
- Author
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Pilar Sanchez-Mosquera, Nuria Macias-Camara, Isabel Lacasa-Viscasillas, Juan L. Iovanna, Carlos Cordon-Cardo, Sara Fernández, Juan M. Falcón-Pérez, José Baselga, Violeta Serra, Erika Castro, Kerman Zorroza, Rocío Montero, Amaia Del Villar, Miguel Unda, Natalia Martín-Martín, Verónica Torrano-Moya, Ainara Egia, Ana M. Aransay, Elena Bilbao, Roberto Bilbao, Eider Salazar, Mireia Castillo-Martin, Amaia Zabala-Letona, Patricia Zúñiga-García, Stéphane Garcia, Jose Tabernero, Edurne Arrieta, Alfredo Caro-Maldonado, Ana Loizaga-Iriarte, Ana R. Cortazar, Guido Cachi-Fuentes, Lorea Valcarcel-Jimenez, Aitziber Ugalde-Olano, Sonia Fernández-Ruiz, Amaia Arruabarrena-Aristorena, Felix Royo, Jorge Gonzalez-Tampan, and Arkaitz Carracedo
- Subjects
Male ,PCA3 ,PTEN ,medicine.disease_cause ,Article ,General Biochemistry, Genetics and Molecular Biology ,Mice ,Prostate cancer ,Prostate ,medicine ,Animals ,Humans ,Gene ,Molecular Biology ,Mutation ,biology ,Biochemistry, Genetics and Molecular Biology(all) ,Tumor Suppressor Proteins ,Fresh tissue ,PTEN Phosphohydrolase ,Prostatic Neoplasms ,Cancer ,Chromoplexy ,medicine.disease ,3. Good health ,medicine.anatomical_structure ,Cancer research ,biology.protein - Abstract
Prostate cancer is among the most frequent cancers in men, and despite its high rate of cure, the high number of cases results in an elevated mortality worldwide. Importantly, prostate cancer incidence is dramatically increasing in western societies in the past decades, suggesting that this type of tumor is exquisitely sensitive to lifestyle changes. Prostate cancer frequently exhibits alterations in the PTEN gene (inactivating mutations or gene deletions) or at the protein level (reduced protein expression or altered sub-cellular compartmentalization). The relevance of PTEN in this type of cancer is further supported by the fact that the sole deletion of PTEN in the murine prostate epithelium recapitulates many of the features of the human disease. In order to study the molecular alterations in prostate cancer, we need to overcome the methodological challenges that this tissue imposes. In this review we present protocols and methods, using PTEN as proof of concept, to study different molecular characteristics of prostate cancer.
- Published
- 2015
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