1. Linker Engineering in the Context of Synthetic Protein Switches and Sensors
- Author
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Viktor Stein and Alexander Gräwe
- Subjects
0301 basic medicine ,Synthetic protein ,Computer science ,Process (engineering) ,Amino Acid Motifs ,Biophysics ,Proteins ,Bioengineering ,Nanotechnology ,Context (language use) ,Biosensing Techniques ,02 engineering and technology ,Protein engineering ,Protein Engineering ,021001 nanoscience & nanotechnology ,03 medical and health sciences ,Synthetic biology ,030104 developmental biology ,Synthetic Biology ,0210 nano-technology ,Actuator ,Linker ,Biotechnology - Abstract
Linkers play critical roles in the construction of synthetic protein switches and sensors as they functionally couple a receptor with an actuator. With an increasing number of molecular toolboxes and experimental strategies becoming available that can be applied to engineer protein switches and sensors with tailored response functions, optimising the connecting linkers remains an idiosyncratic and empiric process. This review aims to provide an in-depth analysis of linker motifs, the biophysical properties they confer, and how they impact the performance of synthetic protein switches and sensors while identifying trends, mechanisms, and strategies that underlie the most potent switches and sensors.
- Published
- 2021
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